Gammaproteobacteria bacterium LSUCC0057

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Porticoccaceae; unclassified Porticoccaceae; SAR92 clade

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1957 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y8UFT2|A0A4Y8UFT2_9GAMM Cytochrome c domain-containing protein OS=Gammaproteobacteria bacterium LSUCC0057 OX=2559237 GN=E3W66_09575 PE=4 SV=1
MM1 pKa = 7.79AIKK4 pKa = 10.56KK5 pKa = 9.73LVLVSAISGLLTACGGGDD23 pKa = 3.77VNISPSNVDD32 pKa = 3.12NSVDD36 pKa = 3.46NSTTTTGGSSSNGFCASYY54 pKa = 10.36TKK56 pKa = 10.48DD57 pKa = 3.08AQLYY61 pKa = 9.0EE62 pKa = 4.44GTLSGGNCVYY72 pKa = 10.39EE73 pKa = 4.44RR74 pKa = 11.84NFVDD78 pKa = 2.93ITNPLTVDD86 pKa = 3.21VTFPSIGSGKK96 pKa = 9.76HH97 pKa = 4.01VFEE100 pKa = 5.2GSLVVGEE107 pKa = 5.11AYY109 pKa = 10.46DD110 pKa = 4.52SIADD114 pKa = 3.83LTAAGITEE122 pKa = 4.59GGDD125 pKa = 3.17GATITIEE132 pKa = 4.03AGNTFVFKK140 pKa = 10.38TSEE143 pKa = 4.08DD144 pKa = 3.75YY145 pKa = 11.36AVINRR150 pKa = 11.84GSQIIAIGDD159 pKa = 3.67AEE161 pKa = 4.25NPITFTSYY169 pKa = 11.02SDD171 pKa = 4.51AISGTVAFDD180 pKa = 5.96DD181 pKa = 4.01VQQWGGMIINGFGVTNKK198 pKa = 9.91CAYY201 pKa = 9.11TGDD204 pKa = 3.41RR205 pKa = 11.84GAADD209 pKa = 3.78FAMVGGEE216 pKa = 4.21CNVAAEE222 pKa = 4.72GKK224 pKa = 9.89SGSAQTYY231 pKa = 9.49YY232 pKa = 11.16GGDD235 pKa = 3.44NDD237 pKa = 4.73ADD239 pKa = 3.88SSGHH243 pKa = 6.06LEE245 pKa = 4.06YY246 pKa = 10.53FIVKK250 pKa = 8.43HH251 pKa = 5.78TGAEE255 pKa = 4.04VAPGNEE261 pKa = 4.04LNGIAFGGVGSGTTVNYY278 pKa = 9.75LQVYY282 pKa = 7.32STYY285 pKa = 11.14DD286 pKa = 3.1DD287 pKa = 4.67GIEE290 pKa = 3.99MFGGSVDD297 pKa = 3.38VSNFVALYY305 pKa = 10.6VRR307 pKa = 11.84DD308 pKa = 4.08DD309 pKa = 3.82SFDD312 pKa = 3.24VDD314 pKa = 3.19EE315 pKa = 5.83GYY317 pKa = 11.24NGTLQNALIIQANDD331 pKa = 3.72DD332 pKa = 3.72GQQCIEE338 pKa = 3.9SDD340 pKa = 4.15GIGSYY345 pKa = 10.78DD346 pKa = 3.42SSEE349 pKa = 4.14TTRR352 pKa = 11.84NADD355 pKa = 4.7FIARR359 pKa = 11.84GLNSAATVNNMTCIVSGNQGGTHH382 pKa = 6.58GDD384 pKa = 3.49SVGVRR389 pKa = 11.84IRR391 pKa = 11.84EE392 pKa = 3.95GHH394 pKa = 7.24AITINDD400 pKa = 4.26SIITSALTNNDD411 pKa = 3.32GLDD414 pKa = 3.22EE415 pKa = 4.35CLRR418 pKa = 11.84IDD420 pKa = 4.98DD421 pKa = 4.79AEE423 pKa = 4.14TRR425 pKa = 11.84QAGFVTITNTLLACKK440 pKa = 10.25KK441 pKa = 9.27PFKK444 pKa = 10.77DD445 pKa = 3.07EE446 pKa = 3.82AVQIGSVGIEE456 pKa = 3.77AWFEE460 pKa = 3.75AADD463 pKa = 3.7GDD465 pKa = 4.31NVVEE469 pKa = 4.38RR470 pKa = 11.84TDD472 pKa = 3.4GAQTITEE479 pKa = 4.12LSGYY483 pKa = 10.14YY484 pKa = 8.81STGDD488 pKa = 3.19RR489 pKa = 11.84GAVTAADD496 pKa = 4.02DD497 pKa = 3.76WTEE500 pKa = 3.82GWTVGVDD507 pKa = 3.79ATWVTPP513 pKa = 3.85

Molecular weight: 53.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y8UH41|A0A4Y8UH41_9GAMM Glucose 1-dehydrogenase OS=Gammaproteobacteria bacterium LSUCC0057 OX=2559237 GN=E3W66_05720 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIKK11 pKa = 10.52RR12 pKa = 11.84KK13 pKa = 8.26RR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.02GRR39 pKa = 11.84KK40 pKa = 8.77RR41 pKa = 11.84LAAA44 pKa = 4.42

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1957

0

1957

679377

38

5070

347.2

37.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.214 ± 0.063

1.078 ± 0.02

5.407 ± 0.051

5.588 ± 0.049

3.4 ± 0.031

7.792 ± 0.054

2.133 ± 0.03

4.967 ± 0.044

2.861 ± 0.042

11.28 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.11 ± 0.029

3.098 ± 0.035

4.513 ± 0.04

5.203 ± 0.053

6.226 ± 0.059

6.24 ± 0.059

4.796 ± 0.056

7.086 ± 0.043

1.348 ± 0.021

2.659 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski