Streptococcus virus Sfi21

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Moineauvirus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 38 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O64277|O64277_9CAUD Orf116 gp OS=Streptococcus virus Sfi21 OX=64186 GN=orf116 PE=4 SV=1
MM1 pKa = 7.32SVSKK5 pKa = 10.12EE6 pKa = 3.79IIMQTLNLDD15 pKa = 3.8EE16 pKa = 4.97TDD18 pKa = 3.78DD19 pKa = 3.8TALIPAYY26 pKa = 9.63IEE28 pKa = 4.23SAQQYY33 pKa = 9.35IINAVGSDD41 pKa = 3.23PKK43 pKa = 10.69FYY45 pKa = 10.69EE46 pKa = 4.2LEE48 pKa = 3.99NVKK51 pKa = 10.63YY52 pKa = 10.77LFDD55 pKa = 3.62TAVIALTSTYY65 pKa = 8.45FTYY68 pKa = 10.15RR69 pKa = 11.84VALNEE74 pKa = 4.01TLTYY78 pKa = 10.07PINLTLNSIIGQLRR92 pKa = 11.84GLYY95 pKa = 8.61ATYY98 pKa = 11.08SDD100 pKa = 4.05GGVNNAA106 pKa = 4.41

Molecular weight: 11.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O21982|O21982_9CAUD Orf288 gp OS=Streptococcus virus Sfi21 OX=64186 GN=orf288 PE=4 SV=1
MM1 pKa = 7.74KK2 pKa = 10.14KK3 pKa = 9.96RR4 pKa = 11.84KK5 pKa = 9.84KK6 pKa = 10.82KK7 pKa = 9.85MINFKK12 pKa = 10.83LRR14 pKa = 11.84LQNKK18 pKa = 6.58ATLVALISAVFLMLQQFGLHH38 pKa = 5.56VPNNIQEE45 pKa = 4.83GINTLVGILVILGIITDD62 pKa = 3.92PTTKK66 pKa = 10.65GIADD70 pKa = 3.71SEE72 pKa = 4.41RR73 pKa = 11.84ALSYY77 pKa = 10.3IQPLDD82 pKa = 3.8DD83 pKa = 4.24KK84 pKa = 11.01EE85 pKa = 4.67VYY87 pKa = 10.08

Molecular weight: 9.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

38

0

38

10808

59

1560

284.4

32.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.551 ± 0.403

0.537 ± 0.104

6.838 ± 0.28

6.162 ± 0.445

4.302 ± 0.17

6.884 ± 0.418

1.443 ± 0.124

6.967 ± 0.274

8.05 ± 0.469

7.652 ± 0.414

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.526 ± 0.202

6.652 ± 0.202

3.063 ± 0.188

4.164 ± 0.246

3.793 ± 0.246

6.393 ± 0.283

6.375 ± 0.249

5.996 ± 0.249

1.554 ± 0.158

4.099 ± 0.329
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski