Photobacterium marinum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Photobacterium

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4904 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L8JK54|L8JK54_9GAMM Arginine/ornithine antiporter ArcD OS=Photobacterium marinum OX=1056511 GN=C942_00187 PE=3 SV=1
MM1 pKa = 8.13AEE3 pKa = 3.52ITLPQGAPSTCKK15 pKa = 10.31AGEE18 pKa = 4.22TVSQKK23 pKa = 11.32LNFKK27 pKa = 8.64VTSTANIRR35 pKa = 11.84YY36 pKa = 8.53NFSFYY41 pKa = 8.48TTTNPLANPLDD52 pKa = 4.16GVPGGNSNEE61 pKa = 3.88CLIWVGEE68 pKa = 4.12VGDD71 pKa = 5.18AGGLPNSQSANGDD84 pKa = 3.22LCADD88 pKa = 3.56VTKK91 pKa = 10.94SQDD94 pKa = 2.66AVYY97 pKa = 9.83QEE99 pKa = 4.13EE100 pKa = 5.11VITFFCQDD108 pKa = 2.75TDD110 pKa = 3.34GDD112 pKa = 4.37SFVDD116 pKa = 4.54LDD118 pKa = 4.6YY119 pKa = 11.48CATWDD124 pKa = 3.83QNDD127 pKa = 4.5DD128 pKa = 3.99NLCSSGGEE136 pKa = 4.07IQSGLLPAPGAPSKK150 pKa = 10.76CNCEE154 pKa = 3.99TVTIPVKK161 pKa = 10.5VLPSEE166 pKa = 4.19PVLEE170 pKa = 4.14KK171 pKa = 10.69AINGDD176 pKa = 3.24ATRR179 pKa = 11.84PEE181 pKa = 3.93SDD183 pKa = 2.77IFAGNDD189 pKa = 3.22SFSFDD194 pKa = 4.33LKK196 pKa = 9.52VTNPNTNTSLVITEE210 pKa = 4.29LVEE213 pKa = 4.09KK214 pKa = 10.76FGGEE218 pKa = 3.84EE219 pKa = 3.92FLISGSLSSSTVSLNEE235 pKa = 3.97DD236 pKa = 3.07QVVLVNAVDD245 pKa = 3.45NDD247 pKa = 3.73GRR249 pKa = 11.84CLASGSEE256 pKa = 4.34TIAPGATYY264 pKa = 10.1ICTVTLRR271 pKa = 11.84WKK273 pKa = 10.77SLILVDD279 pKa = 3.89TDD281 pKa = 3.82SSIEE285 pKa = 3.91GVRR288 pKa = 11.84EE289 pKa = 3.93DD290 pKa = 3.85KK291 pKa = 11.03VSQFGVHH298 pKa = 5.52WKK300 pKa = 10.42FDD302 pKa = 4.4DD303 pKa = 4.59GPEE306 pKa = 4.1AYY308 pKa = 8.91MGPSNSLTVSISDD321 pKa = 3.62VPPIISIAKK330 pKa = 8.04VASPTSIPEE339 pKa = 3.91TGPTGFDD346 pKa = 3.24YY347 pKa = 10.78VHH349 pKa = 6.81YY350 pKa = 10.0YY351 pKa = 8.11VTYY354 pKa = 9.96SNEE357 pKa = 4.23SGWDD361 pKa = 3.48QIHH364 pKa = 6.6VKK366 pKa = 9.47DD367 pKa = 3.92TDD369 pKa = 4.05FIDD372 pKa = 5.12LVQEE376 pKa = 4.09MNTISEE382 pKa = 4.53PKK384 pKa = 8.31PVGYY388 pKa = 8.54TGCAINGLTYY398 pKa = 9.78TQGSGCDD405 pKa = 3.3YY406 pKa = 9.88GVNMGTLYY414 pKa = 11.09SSINAGDD421 pKa = 3.78VYY423 pKa = 11.3KK424 pKa = 10.19NTVKK428 pKa = 10.79VIPTDD433 pKa = 3.44EE434 pKa = 4.57EE435 pKa = 4.65GTSGEE440 pKa = 4.73LKK442 pKa = 9.51TAAASISVEE451 pKa = 3.86NVNPVVTLKK460 pKa = 10.72KK461 pKa = 10.17YY462 pKa = 10.27VRR464 pKa = 11.84AGLAPEE470 pKa = 4.36VNPLDD475 pKa = 3.71PAGYY479 pKa = 9.68HH480 pKa = 7.0DD481 pKa = 5.01MSTSVDD487 pKa = 3.62EE488 pKa = 4.47YY489 pKa = 11.43QLTDD493 pKa = 3.81LSRR496 pKa = 11.84APVVTFLFVVSNSSFEE512 pKa = 4.03NFEE515 pKa = 3.73IMDD518 pKa = 3.96FVDD521 pKa = 4.84FAQAEE526 pKa = 4.72TFGLNPPAQVFDD538 pKa = 4.36SNIADD543 pKa = 3.95TVPVDD548 pKa = 3.98DD549 pKa = 4.7TCSGLIGSSVAVNEE563 pKa = 4.47SVACTMSFKK572 pKa = 10.94VAGDD576 pKa = 4.09DD577 pKa = 4.05SDD579 pKa = 4.82SVDD582 pKa = 3.45NIAWVLVSDD591 pKa = 4.94GEE593 pKa = 4.32QEE595 pKa = 3.98AGGSIGQGFDD605 pKa = 2.93TDD607 pKa = 3.52DD608 pKa = 4.06AMVEE612 pKa = 4.07FNPVGNSIALGLDD625 pKa = 3.19MSATVKK631 pKa = 10.83LSVTAHH637 pKa = 6.6ADD639 pKa = 3.37NVEE642 pKa = 4.14VLSFEE647 pKa = 4.31PLSDD651 pKa = 4.0VIVMVATEE659 pKa = 4.7DD660 pKa = 3.64PNAAEE665 pKa = 4.34TTLDD669 pKa = 3.51EE670 pKa = 4.77PLFSGTFDD678 pKa = 3.0QFTVANIDD686 pKa = 3.77CSSTTIDD693 pKa = 3.03PGYY696 pKa = 9.43TYY698 pKa = 10.86SCSFTFTPKK707 pKa = 10.89GEE709 pKa = 4.2YY710 pKa = 8.61TATAGLQVLNDD721 pKa = 3.8TLKK724 pKa = 10.72VRR726 pKa = 11.84VRR728 pKa = 11.84DD729 pKa = 3.61DD730 pKa = 4.01DD731 pKa = 3.96GTVQEE736 pKa = 4.76LSAVITVEE744 pKa = 3.97AQQ746 pKa = 2.76

Molecular weight: 79.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L8J655|L8J655_9GAMM Uncharacterized protein OS=Photobacterium marinum OX=1056511 GN=C942_02582 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.44NGRR28 pKa = 11.84ATINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.74GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSKK44 pKa = 10.84

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4904

0

4904

1526020

37

2918

311.2

34.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.7 ± 0.038

1.129 ± 0.016

5.533 ± 0.03

6.518 ± 0.036

4.029 ± 0.031

6.863 ± 0.032

2.236 ± 0.019

6.23 ± 0.029

5.266 ± 0.03

10.335 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.775 ± 0.02

4.227 ± 0.025

3.984 ± 0.022

4.507 ± 0.03

4.745 ± 0.03

6.432 ± 0.03

5.256 ± 0.024

6.943 ± 0.031

1.247 ± 0.015

3.045 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski