Paenibacillus phage Shelly

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fernvirus; Paenibacillus virus Shelly

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5AF72|A0A0C5AF72_9CAUD Clp protease-like protein OS=Paenibacillus phage Shelly OX=1589754 GN=SHELLY_4 PE=3 SV=1
MM1 pKa = 7.49KK2 pKa = 10.05IKK4 pKa = 10.36YY5 pKa = 8.81RR6 pKa = 11.84LSIGYY11 pKa = 8.71PGACQEE17 pKa = 4.77DD18 pKa = 4.79EE19 pKa = 4.58IEE21 pKa = 4.69IDD23 pKa = 3.85DD24 pKa = 5.0KK25 pKa = 11.29EE26 pKa = 4.63LEE28 pKa = 4.24GLTPEE33 pKa = 4.11EE34 pKa = 4.18AEE36 pKa = 4.07GRR38 pKa = 11.84ICEE41 pKa = 4.07IVEE44 pKa = 4.33EE45 pKa = 4.26YY46 pKa = 10.89VQDD49 pKa = 5.08FISLSWEE56 pKa = 4.02EE57 pKa = 3.99VEE59 pKa = 4.75EE60 pKa = 4.21

Molecular weight: 7.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5AF59|A0A0C5AF59_9CAUD Uncharacterized protein OS=Paenibacillus phage Shelly OX=1589754 GN=SHELLY_42 PE=4 SV=1
MM1 pKa = 7.29LASIRR6 pKa = 11.84VYY8 pKa = 10.05IQHH11 pKa = 6.86LNSGNPLKK19 pKa = 10.44ATTEE23 pKa = 3.78IEE25 pKa = 4.33RR26 pKa = 11.84KK27 pKa = 9.92KK28 pKa = 9.95KK29 pKa = 8.77TLPSFNPQRR38 pKa = 11.84LRR40 pKa = 3.49

Molecular weight: 4.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

12410

36

878

182.5

20.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.704 ± 0.38

0.862 ± 0.101

5.657 ± 0.223

8.308 ± 0.387

3.409 ± 0.181

6.052 ± 0.377

1.732 ± 0.162

7.051 ± 0.319

8.977 ± 0.341

8.01 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.933 ± 0.18

4.649 ± 0.204

3.239 ± 0.204

4.384 ± 0.224

5.101 ± 0.349

6.43 ± 0.268

5.447 ± 0.243

5.995 ± 0.228

1.418 ± 0.165

3.642 ± 0.227
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski