Paenibacillus crassostreae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4133 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A167FRN6|A0A167FRN6_9BACL Uncharacterized protein OS=Paenibacillus crassostreae OX=1763538 GN=PNBC_05395 PE=4 SV=1
MM1 pKa = 7.72RR2 pKa = 11.84KK3 pKa = 9.62KK4 pKa = 10.42SALIMIMMMLICLPVITSASAVEE27 pKa = 4.36YY28 pKa = 11.22VEE30 pKa = 4.53TPVSKK35 pKa = 9.6EE36 pKa = 3.86TVTAYY41 pKa = 9.78ISNLEE46 pKa = 4.0QQAGHH51 pKa = 6.54LVIDD55 pKa = 3.99ADD57 pKa = 4.16EE58 pKa = 4.36IQWYY62 pKa = 9.5EE63 pKa = 3.32GDD65 pKa = 3.43AAKK68 pKa = 10.6VVFLEE73 pKa = 4.34RR74 pKa = 11.84EE75 pKa = 3.64QDD77 pKa = 3.4TGDD80 pKa = 4.36LEE82 pKa = 4.55GPPDD86 pKa = 4.08GYY88 pKa = 11.38YY89 pKa = 8.83ITNDD93 pKa = 3.14SVEE96 pKa = 4.11EE97 pKa = 3.86VAYY100 pKa = 10.31DD101 pKa = 3.29VSEE104 pKa = 4.07QVEE107 pKa = 4.62VLLQIYY113 pKa = 10.27DD114 pKa = 3.39RR115 pKa = 11.84DD116 pKa = 3.79GTYY119 pKa = 11.0EE120 pKa = 3.98GMDD123 pKa = 3.33IVWNEE128 pKa = 3.96SVTLDD133 pKa = 3.27EE134 pKa = 5.26FKK136 pKa = 10.67TIFEE140 pKa = 4.25NSDD143 pKa = 4.3LIDD146 pKa = 4.34LSQFPYY152 pKa = 10.41HH153 pKa = 7.22LSIQDD158 pKa = 3.47GVVVQIIQQFVPP170 pKa = 3.57

Molecular weight: 19.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A167BI66|A0A167BI66_9BACL Multifunctional 2' 3'-cyclic-nucleotide 2'-phosphodiesterase/5'-nucleotidase/3'-nucleotidase OS=Paenibacillus crassostreae OX=1763538 GN=PNBC_17825 PE=4 SV=1
MM1 pKa = 7.61GPTFKK6 pKa = 10.88PNVSKK11 pKa = 10.73RR12 pKa = 11.84AKK14 pKa = 8.72VHH16 pKa = 5.43GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.48GRR39 pKa = 11.84KK40 pKa = 8.72ILSAA44 pKa = 4.02

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4133

0

4133

1305229

26

2527

315.8

35.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.721 ± 0.037

0.765 ± 0.012

5.353 ± 0.034

6.899 ± 0.041

4.142 ± 0.027

6.919 ± 0.036

2.178 ± 0.02

7.911 ± 0.035

5.74 ± 0.038

9.89 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.983 ± 0.017

4.505 ± 0.031

3.636 ± 0.023

3.979 ± 0.029

4.397 ± 0.029

6.646 ± 0.029

5.524 ± 0.031

7.022 ± 0.031

1.198 ± 0.015

3.591 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski