Serratia ficaria

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Yersiniaceae; Serratia

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4652 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A240C7A6|A0A240C7A6_SERFI Efflux pump membrane transporter OS=Serratia ficaria OX=61651 GN=acrB_2 PE=3 SV=1
MM1 pKa = 7.71SDD3 pKa = 5.02AINKK7 pKa = 9.75CSAQEE12 pKa = 3.75TAACCCVDD20 pKa = 3.6VGTVMDD26 pKa = 4.49NTDD29 pKa = 3.31CTASYY34 pKa = 10.75SQVFGNQQDD43 pKa = 3.79AQAMLAALTAKK54 pKa = 10.19ARR56 pKa = 11.84AVEE59 pKa = 4.47SDD61 pKa = 3.29PCDD64 pKa = 3.07ISSSIKK70 pKa = 10.35PVDD73 pKa = 3.39GGVRR77 pKa = 11.84LAADD81 pKa = 3.93FTFACQAEE89 pKa = 4.33TLIFQLGLRR98 pKa = 4.32

Molecular weight: 10.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A240AFT2|A0A240AFT2_SERFI Elongation factor Tu OS=Serratia ficaria OX=61651 GN=tufA_1 PE=3 SV=1
MM1 pKa = 7.75LDD3 pKa = 3.41SAFVSYY9 pKa = 8.53VTVMSITPGPNNLLLASSGVNFGLRR34 pKa = 11.84RR35 pKa = 11.84TLPMLFGISLGCAVQLALTTSLLALVLSWAGSIRR69 pKa = 11.84FPLAVVGCAYY79 pKa = 10.78LLWLSWKK86 pKa = 9.9LFRR89 pKa = 11.84AGSPDD94 pKa = 3.06AKK96 pKa = 10.26QQARR100 pKa = 11.84PMRR103 pKa = 11.84LLNGALFQAVNPKK116 pKa = 9.77AWLMAINVAILFTPRR131 pKa = 11.84EE132 pKa = 4.29GASLSHH138 pKa = 6.25TLMTIAAFTALNIPCVLVWAILGDD162 pKa = 3.9RR163 pKa = 11.84LRR165 pKa = 11.84DD166 pKa = 3.59ALRR169 pKa = 11.84VGWKK173 pKa = 9.86LRR175 pKa = 11.84LFNGVMSGLMAATALWLLFDD195 pKa = 3.81EE196 pKa = 4.7WRR198 pKa = 11.84AAFAA202 pKa = 4.72

Molecular weight: 21.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4652

0

4652

1501398

29

5951

322.7

35.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.859 ± 0.049

1.023 ± 0.012

5.108 ± 0.026

5.279 ± 0.034

3.797 ± 0.026

7.913 ± 0.035

2.203 ± 0.016

5.328 ± 0.034

3.627 ± 0.031

11.465 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.606 ± 0.016

3.361 ± 0.027

4.722 ± 0.023

4.814 ± 0.034

6.256 ± 0.03

5.641 ± 0.027

4.809 ± 0.018

6.942 ± 0.03

1.434 ± 0.015

2.814 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski