Klebsiella phage YX3973

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411AV26|A0A411AV26_9CAUD Putative type III restriction endonuclease OS=Klebsiella phage YX3973 OX=2500166 PE=4 SV=1
MM1 pKa = 7.78AICANDD7 pKa = 3.25NGIITGRR14 pKa = 11.84QSLIEE19 pKa = 4.23LADD22 pKa = 4.11GCWDD26 pKa = 3.69AAPAEE31 pKa = 4.29EE32 pKa = 4.41DD33 pKa = 3.41WKK35 pKa = 11.07FFAPMTSKK43 pKa = 10.84GVDD46 pKa = 4.16FSPSTTTSEE55 pKa = 4.67ADD57 pKa = 3.5DD58 pKa = 4.34GDD60 pKa = 4.3GFVATLVTTADD71 pKa = 3.67LTISGDD77 pKa = 3.72FEE79 pKa = 4.08VRR81 pKa = 11.84KK82 pKa = 9.9ADD84 pKa = 3.41KK85 pKa = 10.17ADD87 pKa = 3.43EE88 pKa = 4.39YY89 pKa = 11.48GVHH92 pKa = 6.4NLIKK96 pKa = 10.86YY97 pKa = 8.24FVTEE101 pKa = 3.8VKK103 pKa = 10.35ARR105 pKa = 11.84RR106 pKa = 11.84QPSLWVRR113 pKa = 11.84QTTGNTVVVAYY124 pKa = 10.06CNITALSYY132 pKa = 11.28DD133 pKa = 3.64GGTNDD138 pKa = 4.51IITGSVEE145 pKa = 3.78FKK147 pKa = 10.29PYY149 pKa = 10.45DD150 pKa = 3.68GSTVDD155 pKa = 3.28VSSIEE160 pKa = 4.53DD161 pKa = 3.46LTLTTDD167 pKa = 2.85ISKK170 pKa = 8.38TQNVAHH176 pKa = 6.42GTALTLGPVVAAGGVEE192 pKa = 4.27PYY194 pKa = 9.86TYY196 pKa = 10.2QWYY199 pKa = 10.29KK200 pKa = 8.85GTTPISSANTDD211 pKa = 2.8AFTNTNAVASDD222 pKa = 2.94AGTYY226 pKa = 9.15FCRR229 pKa = 11.84VMDD232 pKa = 3.83SATSHH237 pKa = 7.44DD238 pKa = 4.3YY239 pKa = 11.27VDD241 pKa = 3.5STKK244 pKa = 10.77CVVTVTGG251 pKa = 3.92

Molecular weight: 26.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411AV43|A0A411AV43_9CAUD Uncharacterized protein OS=Klebsiella phage YX3973 OX=2500166 PE=4 SV=1
MM1 pKa = 7.36HH2 pKa = 7.31FKK4 pKa = 10.53SLSAIRR10 pKa = 11.84DD11 pKa = 3.43EE12 pKa = 4.49MVGGKK17 pKa = 9.6YY18 pKa = 10.1KK19 pKa = 10.36NQRR22 pKa = 11.84GEE24 pKa = 3.51IAIVVNVVGAGGGYY38 pKa = 8.22QVHH41 pKa = 6.82FNYY44 pKa = 10.31GMPYY48 pKa = 10.51NVVCGLGKK56 pKa = 9.62FRR58 pKa = 11.84KK59 pKa = 9.5RR60 pKa = 11.84YY61 pKa = 6.09PHH63 pKa = 6.81KK64 pKa = 10.88VV65 pKa = 3.04

Molecular weight: 7.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

13458

48

1093

210.3

23.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.489 ± 0.858

1.33 ± 0.19

5.878 ± 0.286

6.739 ± 0.38

3.292 ± 0.22

7.445 ± 0.231

1.702 ± 0.218

6.167 ± 0.231

6.509 ± 0.422

7.208 ± 0.211

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.92 ± 0.178

4.718 ± 0.242

3.544 ± 0.274

3.79 ± 0.422

5.291 ± 0.266

6.502 ± 0.293

5.744 ± 0.484

6.791 ± 0.261

1.397 ± 0.159

3.544 ± 0.25
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski