Pseudomonas phage Psa21

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Phikzvirus; unclassified Phikzvirus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 420 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A481W4V8|A0A481W4V8_9CAUD Uncharacterized protein OS=Pseudomonas phage Psa21 OX=2530023 GN=PSA21_383 PE=4 SV=1
MM1 pKa = 7.8IEE3 pKa = 4.31ALLNFPWKK11 pKa = 10.04IQLGLIWIVLTLLGVWLANNMLNDD35 pKa = 3.38AWRR38 pKa = 11.84NKK40 pKa = 5.84TWCMFIGCFNIFALLLNLLSATVMIVDD67 pKa = 4.1YY68 pKa = 10.95SQEE71 pKa = 3.95QYY73 pKa = 10.85QDD75 pKa = 3.3EE76 pKa = 5.26LFTQEE81 pKa = 4.57LQRR84 pKa = 11.84EE85 pKa = 4.58SIPYY89 pKa = 9.48

Molecular weight: 10.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A481W4L3|A0A481W4L3_9CAUD Uncharacterized protein OS=Pseudomonas phage Psa21 OX=2530023 GN=PSA21_263 PE=4 SV=1
MM1 pKa = 7.62RR2 pKa = 11.84AKK4 pKa = 10.62LKK6 pKa = 10.3DD7 pKa = 3.15IRR9 pKa = 11.84QGRR12 pKa = 11.84TFWEE16 pKa = 4.42VSAYY20 pKa = 10.67LLNRR24 pKa = 11.84DD25 pKa = 3.35MPGFGSITRR34 pKa = 11.84VEE36 pKa = 4.41CLSTPKK42 pKa = 10.77VKK44 pKa = 10.02MLGILGRR51 pKa = 11.84SYY53 pKa = 10.51FANFRR58 pKa = 11.84IEE60 pKa = 4.28TEE62 pKa = 3.92LGSYY66 pKa = 9.41EE67 pKa = 4.36SEE69 pKa = 4.95RR70 pKa = 11.84SLCDD74 pKa = 3.51LNVVHH79 pKa = 7.24FGYY82 pKa = 9.96NQHH85 pKa = 6.8RR86 pKa = 11.84LFRR89 pKa = 11.84KK90 pKa = 9.31KK91 pKa = 9.77KK92 pKa = 8.83HH93 pKa = 5.79AEE95 pKa = 3.68AYY97 pKa = 9.11AARR100 pKa = 11.84LLANCYY106 pKa = 8.78NAEE109 pKa = 3.77EE110 pKa = 4.06RR111 pKa = 11.84RR112 pKa = 11.84FIEE115 pKa = 4.29RR116 pKa = 11.84NGLRR120 pKa = 11.84RR121 pKa = 11.84KK122 pKa = 9.6PSSNSFRR129 pKa = 11.84RR130 pKa = 11.84ASS132 pKa = 3.18

Molecular weight: 15.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

420

0

420

94906

37

2336

226.0

25.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.815 ± 0.201

0.909 ± 0.047

6.244 ± 0.093

6.496 ± 0.13

3.938 ± 0.076

6.177 ± 0.18

2.241 ± 0.08

6.012 ± 0.101

5.654 ± 0.144

8.618 ± 0.099

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.899 ± 0.069

4.881 ± 0.082

4.305 ± 0.08

4.081 ± 0.111

5.175 ± 0.107

5.763 ± 0.08

6.423 ± 0.121

7.277 ± 0.103

1.395 ± 0.044

3.698 ± 0.102
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski