Almpiwar virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Sripuvirus; Almpiwar sripuvirus

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A024A105|A0A024A105_9RHAB Uncharacterized protein OS=Almpiwar virus OX=318843 GN=U1 PE=4 SV=1
MM1 pKa = 7.45SFNDD5 pKa = 3.9FSLTFSPIALKK16 pKa = 10.24AAKK19 pKa = 10.09DD20 pKa = 3.39HH21 pKa = 7.51DD22 pKa = 3.95EE23 pKa = 5.09AEE25 pKa = 4.39INMGTKK31 pKa = 9.9FDD33 pKa = 4.2LEE35 pKa = 4.2NRR37 pKa = 11.84SNQSPTQAFSSSTSEE52 pKa = 3.87EE53 pKa = 3.95VSVEE57 pKa = 4.02KK58 pKa = 9.49TQSSQSVHH66 pKa = 5.24GVEE69 pKa = 4.69FKK71 pKa = 10.5IPRR74 pKa = 11.84VGIPATQQEE83 pKa = 4.6LDD85 pKa = 4.49DD86 pKa = 5.32IIGSVIDD93 pKa = 5.59LVLAQNKK100 pKa = 9.23IEE102 pKa = 4.06HH103 pKa = 6.9HH104 pKa = 7.16DD105 pKa = 3.63IEE107 pKa = 4.68ITDD110 pKa = 3.44KK111 pKa = 10.97AYY113 pKa = 9.71TYY115 pKa = 8.36FIPTSKK121 pKa = 10.13RR122 pKa = 11.84AKK124 pKa = 10.4GYY126 pKa = 7.69DD127 pKa = 3.25TSEE130 pKa = 3.86EE131 pKa = 4.34TFDD134 pKa = 4.31SKK136 pKa = 10.23TPANSEE142 pKa = 3.8EE143 pKa = 4.49DD144 pKa = 3.73EE145 pKa = 4.52EE146 pKa = 6.27ADD148 pKa = 4.9EE149 pKa = 6.07DD150 pKa = 4.15DD151 pKa = 5.05QEE153 pKa = 5.05SDD155 pKa = 3.65SPGSSEE161 pKa = 4.99EE162 pKa = 3.98IDD164 pKa = 3.57SEE166 pKa = 4.79GPSDD170 pKa = 3.53EE171 pKa = 5.87DD172 pKa = 4.02EE173 pKa = 5.16IEE175 pKa = 4.17EE176 pKa = 4.94QEE178 pKa = 4.28GEE180 pKa = 4.22PIKK183 pKa = 11.34GNLEE187 pKa = 3.82KK188 pKa = 10.75LQKK191 pKa = 10.65EE192 pKa = 4.02KK193 pKa = 10.93FGNKK197 pKa = 8.49KK198 pKa = 8.35QAEE201 pKa = 4.28RR202 pKa = 11.84LWRR205 pKa = 11.84SLNEE209 pKa = 4.62GIEE212 pKa = 4.31VPLITGDD219 pKa = 3.34IALVNQDD226 pKa = 3.39NLGITKK232 pKa = 10.19QMLKK236 pKa = 10.43PLDD239 pKa = 4.99FEE241 pKa = 6.31DD242 pKa = 4.35DD243 pKa = 3.47WTPVYY248 pKa = 10.17CLKK251 pKa = 10.82YY252 pKa = 9.25VAFRR256 pKa = 11.84YY257 pKa = 10.17SEE259 pKa = 4.16VCAAGCLIDD268 pKa = 4.08WSDD271 pKa = 3.07ITKK274 pKa = 10.57SS275 pKa = 3.26

Molecular weight:
30.76 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A024A267|A0A024A267_9RHAB Isoform of A0A024A0M0 Protein C OS=Almpiwar virus OX=318843 GN=P PE=4 SV=1
MM1 pKa = 7.23TSLVQSLTWFWLRR14 pKa = 11.84IRR16 pKa = 11.84LNIMILRR23 pKa = 11.84SRR25 pKa = 11.84IKK27 pKa = 9.34HH28 pKa = 5.41TPTSSQHH35 pKa = 6.28LKK37 pKa = 8.13EE38 pKa = 4.31QKK40 pKa = 10.98DD41 pKa = 3.47MTQVRR46 pKa = 11.84RR47 pKa = 11.84LLTQRR52 pKa = 11.84HH53 pKa = 4.71QLTLKK58 pKa = 10.53RR59 pKa = 11.84MKK61 pKa = 10.28KK62 pKa = 7.23QMKK65 pKa = 8.69MIRR68 pKa = 11.84RR69 pKa = 11.84VILLDD74 pKa = 3.69PLKK77 pKa = 10.87KK78 pKa = 10.65LILKK82 pKa = 9.05VRR84 pKa = 11.84LMKK87 pKa = 10.27TRR89 pKa = 11.84LKK91 pKa = 10.47NKK93 pKa = 9.4RR94 pKa = 11.84VNPSRR99 pKa = 11.84AILRR103 pKa = 11.84NCRR106 pKa = 11.84RR107 pKa = 11.84KK108 pKa = 10.19NSEE111 pKa = 3.51TRR113 pKa = 11.84SRR115 pKa = 11.84LKK117 pKa = 10.92DD118 pKa = 3.4CGEE121 pKa = 3.86VLMRR125 pKa = 11.84EE126 pKa = 4.33

Molecular weight:
15.37 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

2

8

3823

79

2095

477.9

54.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.107 ± 0.806

2.066 ± 0.241

5.336 ± 0.522

6.016 ± 0.77

4.813 ± 0.342

5.938 ± 0.447

2.433 ± 0.154

7.743 ± 1.026

7.167 ± 0.363

9.547 ± 0.892

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.668 ± 0.252

5.493 ± 0.538

4.028 ± 0.283

3.139 ± 0.293

4.63 ± 0.622

8.057 ± 0.324

6.121 ± 0.371

4.839 ± 0.334

1.962 ± 0.175

3.897 ± 0.402

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski