Streptococcus phage phiARI0468-4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A141DZK6|A0A141DZK6_9CAUD Uncharacterized protein OS=Streptococcus phage phiARI0468-4 OX=1701830 GN=phiARI0468-4_30 PE=4 SV=1
MM1 pKa = 7.34MEE3 pKa = 4.11EE4 pKa = 4.75LKK6 pKa = 10.89QKK8 pKa = 10.51VNAVYY13 pKa = 10.45NWTVEE18 pKa = 4.16DD19 pKa = 4.66GKK21 pKa = 10.07PKK23 pKa = 10.17PPKK26 pKa = 9.56QDD28 pKa = 2.91LPQAVKK34 pKa = 10.94DD35 pKa = 3.66RR36 pKa = 11.84VDD38 pKa = 3.95YY39 pKa = 9.71FWEE42 pKa = 4.0MAEE45 pKa = 5.33DD46 pKa = 3.88GMTFMGAMEE55 pKa = 5.67CIFADD60 pKa = 4.73EE61 pKa = 5.02KK62 pKa = 10.3PTDD65 pKa = 3.84YY66 pKa = 11.56DD67 pKa = 4.51LGATKK72 pKa = 10.55DD73 pKa = 3.52WLPKK77 pKa = 10.51SKK79 pKa = 10.64EE80 pKa = 3.56FDD82 pKa = 3.09DD83 pKa = 4.59WIGYY87 pKa = 8.14SPSMAQVVIAVYY99 pKa = 10.34LIYY102 pKa = 10.82GGNN105 pKa = 3.26

Molecular weight: 12.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A141DZI8|A0A141DZI8_9CAUD Capsid protein OS=Streptococcus phage phiARI0468-4 OX=1701830 GN=phiARI0468-4_12 PE=4 SV=1
MM1 pKa = 7.5NIAIKK6 pKa = 10.66VDD8 pKa = 3.66LQKK11 pKa = 11.21AKK13 pKa = 10.59QKK15 pKa = 10.71LSNEE19 pKa = 3.7SMTRR23 pKa = 11.84GKK25 pKa = 10.05IAVASKK31 pKa = 10.51ILLDD35 pKa = 3.65NDD37 pKa = 3.28QYY39 pKa = 11.42IPLRR43 pKa = 11.84GGEE46 pKa = 4.01LRR48 pKa = 11.84ASGRR52 pKa = 11.84IVGQGDD58 pKa = 3.52AVVYY62 pKa = 7.1GTVYY66 pKa = 10.93ARR68 pKa = 11.84AQFYY72 pKa = 9.69GANGIVTFRR81 pKa = 11.84RR82 pKa = 11.84YY83 pKa = 5.87TTPGTGKK90 pKa = 10.24RR91 pKa = 11.84WDD93 pKa = 3.44QVATSKK99 pKa = 10.7HH100 pKa = 5.05AEE102 pKa = 3.36EE103 pKa = 4.01WARR106 pKa = 11.84AFVKK110 pKa = 10.83GMGLL114 pKa = 3.32

Molecular weight: 12.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

11401

57

1456

232.7

26.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.008 ± 0.559

0.482 ± 0.065

6.315 ± 0.286

7.35 ± 0.543

4.131 ± 0.294

6.754 ± 0.539

1.5 ± 0.152

6.771 ± 0.296

8.306 ± 0.414

7.429 ± 0.404

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.333 ± 0.189

6.061 ± 0.279

2.824 ± 0.187

4.403 ± 0.322

4.0 ± 0.224

6.228 ± 0.424

6.613 ± 0.483

6.184 ± 0.245

1.202 ± 0.128

4.105 ± 0.309
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski