Bifidobacterium phage Bbif-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I3NLD4|I3NLD4_9CAUD RlfA phagic protein OS=Bifidobacterium phage Bbif-1 OX=743964 GN=BBP_0971 PE=4 SV=1
MM1 pKa = 7.17EE2 pKa = 6.25HH3 pKa = 6.58IASTFGLTSLDD14 pKa = 3.0IYY16 pKa = 10.66TRR18 pKa = 11.84ALGSDD23 pKa = 3.36AARR26 pKa = 11.84AYY28 pKa = 8.0EE29 pKa = 3.79ARR31 pKa = 11.84EE32 pKa = 3.96RR33 pKa = 11.84EE34 pKa = 4.24FQVTDD39 pKa = 3.81DD40 pKa = 4.91LIDD43 pKa = 5.06RR44 pKa = 11.84IAAHH48 pKa = 6.81PEE50 pKa = 4.11DD51 pKa = 4.59YY52 pKa = 10.94DD53 pKa = 3.74VAANRR58 pKa = 11.84DD59 pKa = 3.43PNARR63 pKa = 11.84LEE65 pKa = 4.32AEE67 pKa = 4.55TPDD70 pKa = 3.59EE71 pKa = 4.15

Molecular weight: 7.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I3NLE0|I3NLE0_9CAUD Uncharacterized protein OS=Bifidobacterium phage Bbif-1 OX=743964 GN=BBP_0977 PE=4 SV=1
MM1 pKa = 7.52GIEE4 pKa = 4.15YY5 pKa = 9.59MSVTDD10 pKa = 3.38VAKK13 pKa = 10.69RR14 pKa = 11.84LGISTAAASAYY25 pKa = 9.68KK26 pKa = 10.37LPQPDD31 pKa = 3.16ATIGRR36 pKa = 11.84TRR38 pKa = 11.84GWLPEE43 pKa = 4.39TIDD46 pKa = 2.92RR47 pKa = 11.84WNASRR52 pKa = 11.84PGRR55 pKa = 11.84GVGGGRR61 pKa = 11.84PRR63 pKa = 11.84KK64 pKa = 9.65NKK66 pKa = 10.29NEE68 pKa = 3.84

Molecular weight: 7.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

11811

41

1044

193.6

21.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.354 ± 0.632

1.118 ± 0.17

7.18 ± 0.275

5.698 ± 0.353

2.1 ± 0.132

8.179 ± 0.447

1.981 ± 0.219

4.995 ± 0.237

4.326 ± 0.255

8.052 ± 0.222

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.692 ± 0.229

3.26 ± 0.196

4.36 ± 0.286

3.734 ± 0.259

7.755 ± 0.49

5.757 ± 0.328

7.476 ± 0.423

5.469 ± 0.306

2.091 ± 0.239

2.421 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski