Klebsiella phage 48ST307

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z2EKP6|A0A2Z2EKP6_9CAUD DUF1983 domain-containing protein OS=Klebsiella phage 48ST307 OX=1955242 PE=4 SV=1
MM1 pKa = 7.23FCADD5 pKa = 4.25GEE7 pKa = 4.42NSAEE11 pKa = 4.41VYY13 pKa = 10.7CGATTMAQAKK23 pKa = 9.81KK24 pKa = 10.13VFTPARR30 pKa = 11.84QMASRR35 pKa = 11.84LPALRR40 pKa = 11.84SRR42 pKa = 11.84FDD44 pKa = 3.08ISVWTDD50 pKa = 2.52SLTRR54 pKa = 11.84PDD56 pKa = 3.85GSVFAPMAGKK66 pKa = 9.34PGDD69 pKa = 4.0GDD71 pKa = 4.02SPHH74 pKa = 6.8CAIIDD79 pKa = 3.92EE80 pKa = 4.45YY81 pKa = 11.24HH82 pKa = 6.38EE83 pKa = 5.19HH84 pKa = 6.91DD85 pKa = 3.78TDD87 pKa = 3.67HH88 pKa = 6.81MYY90 pKa = 11.12EE91 pKa = 3.95AMTMGMGARR100 pKa = 11.84SQPLTLIITTAGSSLEE116 pKa = 4.17SPCYY120 pKa = 10.68DD121 pKa = 3.04KK122 pKa = 11.39DD123 pKa = 4.18KK124 pKa = 10.73EE125 pKa = 4.36VKK127 pKa = 9.94EE128 pKa = 4.17VIEE131 pKa = 4.94GITRR135 pKa = 11.84NDD137 pKa = 3.29RR138 pKa = 11.84LFGMIYY144 pKa = 10.26EE145 pKa = 5.0LDD147 pKa = 4.53AGDD150 pKa = 5.23DD151 pKa = 3.4WTDD154 pKa = 3.32PKK156 pKa = 11.43NLIKK160 pKa = 10.8ANPNLDD166 pKa = 3.39VSVKK170 pKa = 10.55YY171 pKa = 10.59SDD173 pKa = 4.15LVEE176 pKa = 4.2LLEE179 pKa = 4.38VAKK182 pKa = 10.21QVPRR186 pKa = 11.84KK187 pKa = 10.19VNAFKK192 pKa = 10.48TKK194 pKa = 10.31RR195 pKa = 11.84LNIWVSGKK203 pKa = 9.62SAFYY207 pKa = 11.32NMEE210 pKa = 3.53QWKK213 pKa = 9.72AAEE216 pKa = 4.52DD217 pKa = 4.18PNLEE221 pKa = 4.3LADD224 pKa = 4.47FANDD228 pKa = 3.19SCNIGLDD235 pKa = 3.72LAKK238 pKa = 10.67KK239 pKa = 10.48LDD241 pKa = 3.71MNAGIRR247 pKa = 11.84LFTRR251 pKa = 11.84EE252 pKa = 3.9IEE254 pKa = 4.54GKK256 pKa = 9.13RR257 pKa = 11.84HH258 pKa = 5.31YY259 pKa = 11.03YY260 pKa = 9.68CIKK263 pKa = 9.43PKK265 pKa = 10.34FWVPEE270 pKa = 4.11DD271 pKa = 4.29TIHH274 pKa = 5.58TTDD277 pKa = 3.56PKK279 pKa = 10.94LLKK282 pKa = 9.73TADD285 pKa = 3.97RR286 pKa = 11.84YY287 pKa = 10.26QKK289 pKa = 9.95FYY291 pKa = 11.35EE292 pKa = 4.15MGVLEE297 pKa = 4.53ATDD300 pKa = 3.79GAEE303 pKa = 3.6ADD305 pKa = 3.69YY306 pKa = 11.36RR307 pKa = 11.84EE308 pKa = 4.26ILASIIDD315 pKa = 3.84MQDD318 pKa = 2.67EE319 pKa = 4.12NRR321 pKa = 11.84IDD323 pKa = 4.64EE324 pKa = 4.39IDD326 pKa = 3.76IDD328 pKa = 4.19PAGATALRR336 pKa = 11.84HH337 pKa = 4.74QLEE340 pKa = 4.81DD341 pKa = 3.17NGFTVVDD348 pKa = 3.54IRR350 pKa = 11.84QDD352 pKa = 3.6YY353 pKa = 9.7TNMSPAMKK361 pKa = 9.91EE362 pKa = 3.83LEE364 pKa = 4.0AALAGGRR371 pKa = 11.84FHH373 pKa = 7.84HH374 pKa = 7.44DD375 pKa = 3.32GNPILTWCISNVIGKK390 pKa = 7.4FIPGSDD396 pKa = 3.41DD397 pKa = 3.2LVRR400 pKa = 11.84PTKK403 pKa = 10.66GDD405 pKa = 3.49NQSKK409 pKa = 10.31IDD411 pKa = 3.58GATALFNAMTRR422 pKa = 11.84AMLHH426 pKa = 6.02EE427 pKa = 4.75SSGGTSVYY435 pKa = 10.66DD436 pKa = 4.16EE437 pKa = 4.82EE438 pKa = 7.47DD439 pKa = 3.34IACC442 pKa = 4.51

Molecular weight: 49.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z2EN75|A0A2Z2EN75_9CAUD Uncharacterized protein OS=Klebsiella phage 48ST307 OX=1955242 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84YY3 pKa = 9.47SRR5 pKa = 11.84SEE7 pKa = 3.62ARR9 pKa = 11.84GVIMNSLKK17 pKa = 10.67RR18 pKa = 11.84MAKK21 pKa = 9.82AWLLMNGAFVLLVMATQPAMANEE44 pKa = 4.19SQSLGLDD51 pKa = 3.19LDD53 pKa = 5.0SILSALPAGWASGVTAVFIVLYY75 pKa = 10.65AVAQLRR81 pKa = 11.84AVLPPSVTKK90 pKa = 10.82RR91 pKa = 11.84IPTVVMKK98 pKa = 10.78ILDD101 pKa = 3.89LVAANYY107 pKa = 9.88AHH109 pKa = 7.12ARR111 pKa = 11.84NADD114 pKa = 4.16AISKK118 pKa = 7.93VARR121 pKa = 11.84DD122 pKa = 3.38AGKK125 pKa = 10.49AKK127 pKa = 10.44GPSDD131 pKa = 2.95VDD133 pKa = 3.23YY134 pKa = 11.1RR135 pKa = 11.84VMVEE139 pKa = 3.78TAKK142 pKa = 11.17NNGEE146 pKa = 3.95LRR148 pKa = 11.84GSRR151 pKa = 11.84IEE153 pKa = 4.21SAGDD157 pKa = 3.41YY158 pKa = 10.78PGDD161 pKa = 4.03DD162 pKa = 3.63RR163 pKa = 11.84PGSKK167 pKa = 10.13SPQQ170 pKa = 3.11

Molecular weight: 18.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24

0

24

12793

129

3193

533.0

58.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.177 ± 0.537

0.883 ± 0.19

5.925 ± 0.264

5.605 ± 0.518

3.088 ± 0.315

7.574 ± 0.408

1.423 ± 0.291

5.37 ± 0.295

5.143 ± 0.505

8.45 ± 0.434

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.415 ± 0.338

5.042 ± 0.455

3.572 ± 0.305

4.823 ± 0.366

5.495 ± 0.405

7.41 ± 0.695

6.98 ± 0.839

6.207 ± 0.367

1.438 ± 0.198

2.978 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski