Escherichia phage Lilleput

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Bullavirinae; unclassified Bullavirinae

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6DZ07|A0A4D6DZ07_9VIRU H protein OS=Escherichia phage Lilleput OX=2562232 PE=4 SV=1
MM1 pKa = 7.53NSNEE5 pKa = 4.22SAVAFQTAIASIKK18 pKa = 9.2MIQASSVLDD27 pKa = 3.53LTEE30 pKa = 5.82DD31 pKa = 3.47DD32 pKa = 5.78FEE34 pKa = 4.82FLTGDD39 pKa = 3.9RR40 pKa = 11.84VWIATDD46 pKa = 3.49RR47 pKa = 11.84SRR49 pKa = 11.84ARR51 pKa = 11.84RR52 pKa = 11.84AVEE55 pKa = 3.52ACVYY59 pKa = 8.38GTLDD63 pKa = 3.27FVGYY67 pKa = 9.24PRR69 pKa = 11.84FPAPVEE75 pKa = 4.42FISAVIAYY83 pKa = 7.79YY84 pKa = 8.7VHH86 pKa = 6.96PVNIQTACLIMEE98 pKa = 4.57GAEE101 pKa = 3.88FTEE104 pKa = 4.67NIVNGVEE111 pKa = 4.44SPVKK115 pKa = 10.44ASEE118 pKa = 3.96LFAFVLRR125 pKa = 11.84VRR127 pKa = 11.84AGCKK131 pKa = 9.8DD132 pKa = 3.6VLEE135 pKa = 4.51GAEE138 pKa = 3.99DD139 pKa = 3.92NIRR142 pKa = 11.84AKK144 pKa = 10.71LRR146 pKa = 11.84AQGIMM151 pKa = 3.61

Molecular weight: 16.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6DYW2|A0A4D6DYW2_9VIRU External scaffolding protein D OS=Escherichia phage Lilleput OX=2562232 PE=3 SV=1
MM1 pKa = 7.44SVEE4 pKa = 4.3LIKK7 pKa = 10.7PLVEE11 pKa = 3.86AAGMSTLTQSPNLRR25 pKa = 11.84IWKK28 pKa = 8.93QVNTRR33 pKa = 11.84VKK35 pKa = 10.51LLEE38 pKa = 4.88EE39 pKa = 4.62ILAHH43 pKa = 5.1WTNGIRR49 pKa = 11.84RR50 pKa = 11.84CPEE53 pKa = 3.56TQDD56 pKa = 4.78FYY58 pKa = 11.45MNPNSQLATTIAYY71 pKa = 8.04RR72 pKa = 11.84AHH74 pKa = 6.19QKK76 pKa = 9.51GHH78 pKa = 5.39NPKK81 pKa = 10.28LIQYY85 pKa = 8.27PDD87 pKa = 3.65TFTLDD92 pKa = 3.71DD93 pKa = 4.6IIRR96 pKa = 11.84RR97 pKa = 11.84KK98 pKa = 9.8PIPQTAPNEE107 pKa = 3.94LRR109 pKa = 11.84LSDD112 pKa = 5.03EE113 pKa = 3.69IGEE116 pKa = 4.37DD117 pKa = 3.51YY118 pKa = 10.75RR119 pKa = 11.84LTVIAIADD127 pKa = 4.11EE128 pKa = 4.23LQEE131 pKa = 4.7CYY133 pKa = 10.51DD134 pKa = 3.64VLGQLDD140 pKa = 3.83INNTIDD146 pKa = 3.4HH147 pKa = 6.15TPKK150 pKa = 10.95GNAHH154 pKa = 6.5WSMLYY159 pKa = 10.22DD160 pKa = 4.05KK161 pKa = 10.32PVNTHH166 pKa = 5.13WSQLVSKK173 pKa = 10.58RR174 pKa = 11.84PLKK177 pKa = 10.43DD178 pKa = 2.77IRR180 pKa = 11.84ADD182 pKa = 3.47YY183 pKa = 10.94NYY185 pKa = 11.09AKK187 pKa = 10.74AKK189 pKa = 9.85GIKK192 pKa = 9.69DD193 pKa = 3.59EE194 pKa = 4.13CSKK197 pKa = 10.4MLEE200 pKa = 4.05EE201 pKa = 4.36STMKK205 pKa = 10.09SRR207 pKa = 11.84RR208 pKa = 11.84GFTVQRR214 pKa = 11.84LMNAMRR220 pKa = 11.84QAHH223 pKa = 5.52TDD225 pKa = 2.56GWYY228 pKa = 10.64VVFDD232 pKa = 4.23TLTLADD238 pKa = 4.35DD239 pKa = 5.61RR240 pKa = 11.84IQAFYY245 pKa = 11.18DD246 pKa = 3.46NPNALRR252 pKa = 11.84DD253 pKa = 3.67YY254 pKa = 10.67FRR256 pKa = 11.84DD257 pKa = 3.04IGRR260 pKa = 11.84MVLAAEE266 pKa = 4.55GRR268 pKa = 11.84SANDD272 pKa = 3.16SHH274 pKa = 7.55SDD276 pKa = 3.48CYY278 pKa = 11.08QYY280 pKa = 11.34FCVPEE285 pKa = 4.02YY286 pKa = 9.72GTQHH290 pKa = 5.68GRR292 pKa = 11.84LHH294 pKa = 5.96FHH296 pKa = 6.87AVHH299 pKa = 6.99LMRR302 pKa = 11.84TLPLGSRR309 pKa = 11.84DD310 pKa = 3.68PNFGKK315 pKa = 10.52LVRR318 pKa = 11.84TNRR321 pKa = 11.84QINSLQNTWPYY332 pKa = 9.75GYY334 pKa = 10.78SMPIAVRR341 pKa = 11.84YY342 pKa = 9.26SQDD345 pKa = 2.82AFSRR349 pKa = 11.84AGWLWPVDD357 pKa = 4.15SKK359 pKa = 11.92GEE361 pKa = 4.01PLKK364 pKa = 10.22ATSYY368 pKa = 9.01MAVGFYY374 pKa = 9.16VAKK377 pKa = 10.34YY378 pKa = 9.45VNKK381 pKa = 10.55KK382 pKa = 10.41SDD384 pKa = 2.79IDD386 pKa = 3.67MAAKK390 pKa = 10.6GLGNKK395 pKa = 7.49EE396 pKa = 3.68WNASLKK402 pKa = 9.91TKK404 pKa = 10.52ISLLPTKK411 pKa = 10.29LFRR414 pKa = 11.84IRR416 pKa = 11.84MSRR419 pKa = 11.84NFGMKK424 pKa = 8.53MLSMAHH430 pKa = 5.91LTAEE434 pKa = 4.48ALIQLTQLGYY444 pKa = 11.13DD445 pKa = 3.19STPFNNVLKK454 pKa = 10.9QNAKK458 pKa = 10.35KK459 pKa = 9.92EE460 pKa = 4.32LKK462 pKa = 10.35SRR464 pKa = 11.84LAKK467 pKa = 10.68ASVATVLAAQPVTTNLLKK485 pKa = 10.7FMRR488 pKa = 11.84DD489 pKa = 3.15LTRR492 pKa = 11.84TIGASNLQNFIASMTQKK509 pKa = 9.35LTLMDD514 pKa = 3.89TSDD517 pKa = 3.28EE518 pKa = 4.41TKK520 pKa = 10.85NYY522 pKa = 9.64LAAAGITTACLRR534 pKa = 11.84IKK536 pKa = 10.66SKK538 pKa = 8.22WTAGGKK544 pKa = 9.39

Molecular weight: 61.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1728

86

544

288.0

31.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.722 ± 0.932

0.868 ± 0.191

5.845 ± 0.322

3.704 ± 0.598

3.704 ± 0.499

6.481 ± 0.806

2.083 ± 0.559

5.382 ± 0.434

5.498 ± 0.925

8.275 ± 0.932

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.951 ± 0.42

5.382 ± 0.283

4.225 ± 0.478

5.093 ± 0.705

5.093 ± 0.707

7.292 ± 0.758

7.813 ± 0.843

5.729 ± 0.861

1.331 ± 0.27

3.53 ± 0.596
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski