Pseudoalteromonas phage PM2 (Bacteriophage PM2)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes; Vinavirales; Corticoviridae; Corticovirus

Average proteome isoelectric point is 7.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9XJR5|P8_BPPM2 Protein P8 OS=Pseudoalteromonas phage PM2 OX=10661 GN=VIII PE=1 SV=1
MM1 pKa = 7.42GLFGGGNSKK10 pKa = 8.78STSNQTTNNEE20 pKa = 3.93NTNIATQGDD29 pKa = 3.83NLGAVINGNGNSVTMTDD46 pKa = 3.48HH47 pKa = 6.6GLVDD51 pKa = 3.98ALVDD55 pKa = 3.18IGGYY59 pKa = 9.31MSDD62 pKa = 3.17STQAAFGAASDD73 pKa = 3.77MAYY76 pKa = 10.74SSTEE80 pKa = 3.65FAGQAITDD88 pKa = 3.78GFDD91 pKa = 3.09YY92 pKa = 11.48AEE94 pKa = 4.28GVNRR98 pKa = 11.84DD99 pKa = 3.93SLDD102 pKa = 3.17MAEE105 pKa = 4.81GINRR109 pKa = 11.84DD110 pKa = 3.38SLNFGRR116 pKa = 11.84DD117 pKa = 3.44ALSVTGDD124 pKa = 3.47LMTDD128 pKa = 3.25AMQYY132 pKa = 10.92SSDD135 pKa = 3.54AMLASIEE142 pKa = 4.31GNAGLAGQVMDD153 pKa = 5.21ASTTMTGQSLNFGLDD168 pKa = 3.41TFSGAMDD175 pKa = 4.72SLNQSNNNMALLAEE189 pKa = 4.55FTSNQSTDD197 pKa = 3.25LARR200 pKa = 11.84DD201 pKa = 3.41SMAFGADD208 pKa = 4.62LMAQYY213 pKa = 10.6QDD215 pKa = 4.3NISASNYY222 pKa = 8.66DD223 pKa = 3.5ARR225 pKa = 11.84EE226 pKa = 3.96HH227 pKa = 6.24MLDD230 pKa = 3.3ASKK233 pKa = 9.19TAMQFADD240 pKa = 3.2NMSRR244 pKa = 11.84SDD246 pKa = 3.69GQQLAKK252 pKa = 10.69DD253 pKa = 3.85SNKK256 pKa = 9.27TLMIGIVAVSAAVGLYY272 pKa = 10.45AISKK276 pKa = 9.22GVNN279 pKa = 2.97

Molecular weight: 29.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q9XJR9|P9_BPPM2 Protein P9 OS=Pseudoalteromonas phage PM2 OX=10661 GN=IX PE=1 SV=2
MM1 pKa = 7.49INKK4 pKa = 7.38TTIKK8 pKa = 9.8TVLITLGVLAAVNKK22 pKa = 9.54VSALRR27 pKa = 11.84SVKK30 pKa = 10.4RR31 pKa = 11.84LISS34 pKa = 3.49

Molecular weight: 3.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21

1

22

3156

30

634

143.5

15.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.252 ± 0.603

0.887 ± 0.28

6.115 ± 0.771

6.274 ± 0.617

3.802 ± 0.292

7.003 ± 0.759

1.394 ± 0.168

5.894 ± 0.389

7.129 ± 0.77

8.809 ± 0.649

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.105 ± 0.397

5.292 ± 0.491

3.074 ± 0.426

4.024 ± 0.362

4.943 ± 0.503

6.21 ± 0.571

5.767 ± 0.403

6.781 ± 0.681

1.267 ± 0.177

2.978 ± 0.372
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski