Bos taurus (Bovine)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria;

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37882 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|A8QW39|F183A_BOVIN Protein FAM183A OS=Bos taurus OX=9913 GN=FAM183A PE=2 SV=1
MM1 pKa = 8.24DD2 pKa = 4.75FQQLADD8 pKa = 4.22VAEE11 pKa = 4.71KK12 pKa = 9.75WCSNTPFEE20 pKa = 5.73LIATEE25 pKa = 3.96EE26 pKa = 4.32TEE28 pKa = 4.33RR29 pKa = 11.84RR30 pKa = 11.84MDD32 pKa = 4.29FYY34 pKa = 11.47ADD36 pKa = 3.74PGVSFYY42 pKa = 10.98VLCPDD47 pKa = 4.64NGCGDD52 pKa = 3.96NFHH55 pKa = 6.3VWSEE59 pKa = 4.48SEE61 pKa = 3.97DD62 pKa = 3.63CLPFLQLAQDD72 pKa = 4.45YY73 pKa = 10.71ISSCGKK79 pKa = 8.56KK80 pKa = 7.94TLHH83 pKa = 6.31EE84 pKa = 4.11VLEE87 pKa = 4.47KK88 pKa = 10.61VFKK91 pKa = 10.63SFRR94 pKa = 11.84PLLGLPDD101 pKa = 4.83ADD103 pKa = 3.93DD104 pKa = 4.59DD105 pKa = 4.62AFEE108 pKa = 4.77EE109 pKa = 4.52YY110 pKa = 10.51SADD113 pKa = 3.68VEE115 pKa = 4.38EE116 pKa = 5.09EE117 pKa = 4.2EE118 pKa = 5.29PEE120 pKa = 4.13ADD122 pKa = 3.66HH123 pKa = 6.65PQMGVSQQQ131 pKa = 3.0

Molecular weight: 14.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q1LRN5|A0A3Q1LRN5_BOVIN Uncharacterized protein OS=Bos taurus OX=9913 GN=ZNF454 PE=4 SV=1
RR1 pKa = 7.4SPHH4 pKa = 4.88TAGRR8 pKa = 11.84SPHH11 pKa = 5.18TAGRR15 pKa = 11.84SPHH18 pKa = 5.18TAGRR22 pKa = 11.84SPHH25 pKa = 5.18TAGRR29 pKa = 11.84SPHH32 pKa = 5.18TAGRR36 pKa = 11.84SPHH39 pKa = 5.18TAGRR43 pKa = 11.84SPHH46 pKa = 4.96TRR48 pKa = 11.84VRR50 pKa = 11.84TPHH53 pKa = 5.34TAGRR57 pKa = 11.84SPHH60 pKa = 5.18TAGRR64 pKa = 11.84SPHH67 pKa = 5.18TAGRR71 pKa = 11.84SPHH74 pKa = 5.61TAGGSPHH81 pKa = 5.85TAGRR85 pKa = 11.84SPHH88 pKa = 4.85TAGRR92 pKa = 11.84IRR94 pKa = 11.84TPHH97 pKa = 5.21TAGRR101 pKa = 11.84SPHH104 pKa = 5.18TAGRR108 pKa = 11.84SPHH111 pKa = 5.61TAGGSPHH118 pKa = 6.22TRR120 pKa = 11.84VKK122 pKa = 10.06TPHH125 pKa = 5.45TAGRR129 pKa = 11.84SPHH132 pKa = 5.18TAGRR136 pKa = 11.84SPHH139 pKa = 5.18TAGRR143 pKa = 11.84SPHH146 pKa = 4.96TRR148 pKa = 11.84VRR150 pKa = 11.84TPHH153 pKa = 5.34TAGRR157 pKa = 11.84SPHH160 pKa = 5.18TAGRR164 pKa = 11.84SPHH167 pKa = 4.96TQGEE171 pKa = 4.33AFLL174 pKa = 5.21

Molecular weight: 17.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23847

14035

37882

22676720

8

8139

598.6

66.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.059 ± 0.014

2.228 ± 0.013

4.845 ± 0.009

7.069 ± 0.016

3.653 ± 0.009

6.577 ± 0.017

2.536 ± 0.006

4.379 ± 0.011

5.684 ± 0.014

10.0 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.124 ± 0.006

3.558 ± 0.009

6.317 ± 0.019

4.721 ± 0.01

5.719 ± 0.012

8.31 ± 0.017

5.277 ± 0.01

6.069 ± 0.011

1.227 ± 0.005

2.648 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski