Geothermobacter sp. EPR-M

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfuromonadales; Geobacteraceae; Geothermobacter

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3263 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X0YAF8|A0A1X0YAF8_9DELT Uncharacterized protein OS=Geothermobacter sp. EPR-M OX=1969733 GN=B5V00_04800 PE=4 SV=1
MM1 pKa = 7.69LGLDD5 pKa = 3.19YY6 pKa = 11.32SAFNTNGEE14 pKa = 4.25LDD16 pKa = 4.55LYY18 pKa = 11.21DD19 pKa = 4.31PSTGKK24 pKa = 10.6GRR26 pKa = 11.84LTNMYY31 pKa = 9.87LADD34 pKa = 4.1RR35 pKa = 11.84AEE37 pKa = 4.07YY38 pKa = 10.12LANLLYY44 pKa = 10.99ANSNDD49 pKa = 3.77TTDD52 pKa = 3.06SGTDD56 pKa = 3.23VQYY59 pKa = 11.61VDD61 pKa = 3.89LSDD64 pKa = 4.84RR65 pKa = 11.84VTLNQPLLLNPEE77 pKa = 4.23KK78 pKa = 10.52KK79 pKa = 10.06ISFGTDD85 pKa = 2.61TADD88 pKa = 3.69LLEE91 pKa = 4.95GSWSVLAGDD100 pKa = 3.81NVVDD104 pKa = 3.98HH105 pKa = 6.91LYY107 pKa = 11.52GMGGNDD113 pKa = 4.42AMQHH117 pKa = 5.62CVNIDD122 pKa = 2.97AA123 pKa = 5.5

Molecular weight: 13.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X0XZH5|A0A1X0XZH5_9DELT tRNA modification GTPase MnmE OS=Geothermobacter sp. EPR-M OX=1969733 GN=mnmE PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 9.9QPSRR9 pKa = 11.84ISRR12 pKa = 11.84KK13 pKa = 7.55RR14 pKa = 11.84THH16 pKa = 6.52GFRR19 pKa = 11.84KK20 pKa = 10.07RR21 pKa = 11.84MQSKK25 pKa = 9.99NGQSVIRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.12GRR39 pKa = 11.84KK40 pKa = 6.49TLAATIPSKK49 pKa = 11.12

Molecular weight: 5.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3263

0

3263

1086227

37

3358

332.9

36.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.516 ± 0.051

1.315 ± 0.022

5.608 ± 0.035

6.478 ± 0.051

3.913 ± 0.027

8.208 ± 0.048

2.047 ± 0.023

5.446 ± 0.033

3.946 ± 0.04

11.544 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.223 ± 0.021

3.012 ± 0.028

4.803 ± 0.035

3.817 ± 0.027

7.355 ± 0.055

5.316 ± 0.03

4.823 ± 0.037

6.987 ± 0.039

1.087 ± 0.019

2.556 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski