Megasphaera sp. UPII 135-E

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Veillonellales; Veillonellaceae; Megasphaera; unclassified Megasphaera

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1512 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F9MQL0|F9MQL0_9FIRM UvrABC system protein A OS=Megasphaera sp. UPII 135-E OX=1000569 GN=uvrA PE=3 SV=1
MM1 pKa = 8.05DD2 pKa = 5.19KK3 pKa = 11.22YY4 pKa = 10.8EE5 pKa = 4.4CTVCGYY11 pKa = 10.14IYY13 pKa = 10.76DD14 pKa = 4.11EE15 pKa = 5.33AEE17 pKa = 3.85GDD19 pKa = 3.58ADD21 pKa = 3.79NGISAGTKK29 pKa = 9.77FADD32 pKa = 4.75LAPDD36 pKa = 4.11WMCPMCGADD45 pKa = 3.26KK46 pKa = 11.18GSFVKK51 pKa = 10.48VDD53 pKa = 3.1

Molecular weight: 5.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F9MQC3|F9MQC3_9FIRM tRNA dimethylallyltransferase OS=Megasphaera sp. UPII 135-E OX=1000569 GN=miaA PE=3 SV=1
MM1 pKa = 7.11LTHH4 pKa = 7.31GIAGCIPCFFSAGLVSLVLGNEE26 pKa = 3.95RR27 pKa = 11.84NTGVFIALRR36 pKa = 11.84MLAYY40 pKa = 9.44TFLFILKK47 pKa = 10.14ISIKK51 pKa = 10.26RR52 pKa = 11.84RR53 pKa = 3.34

Molecular weight: 5.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1512

0

1512

491780

37

4354

325.3

36.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.067 ± 0.094

1.311 ± 0.033

5.21 ± 0.084

6.287 ± 0.08

3.693 ± 0.053

7.329 ± 0.109

2.316 ± 0.04

7.696 ± 0.078

6.581 ± 0.096

9.032 ± 0.089

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.911 ± 0.035

3.9 ± 0.088

3.54 ± 0.051

4.201 ± 0.056

4.65 ± 0.075

5.374 ± 0.05

5.91 ± 0.084

7.324 ± 0.057

0.963 ± 0.025

3.705 ± 0.059
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski