Clostridium sp. CAG:62

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2452 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7C9Q2|R7C9Q2_9CLOT Serine-type D-Ala-D-Ala carboxypeptidase OS=Clostridium sp. CAG:62 OX=1262828 GN=BN737_00057 PE=3 SV=1
MM1 pKa = 7.41AVSSIGDD8 pKa = 3.65TYY10 pKa = 9.16FTNYY14 pKa = 8.14STEE17 pKa = 4.01ANDD20 pKa = 4.14AVSQKK25 pKa = 7.79MTSQIQDD32 pKa = 3.1AEE34 pKa = 4.33TDD36 pKa = 3.52EE37 pKa = 4.59EE38 pKa = 4.43MLDD41 pKa = 3.23ACKK44 pKa = 10.21QFEE47 pKa = 4.63SYY49 pKa = 10.58LVQQMFKK56 pKa = 11.05SMEE59 pKa = 3.78EE60 pKa = 4.05SAKK63 pKa = 10.47VFSDD67 pKa = 4.21DD68 pKa = 6.39DD69 pKa = 4.2EE70 pKa = 6.63DD71 pKa = 4.34NSNDD75 pKa = 3.89YY76 pKa = 11.85VNMFQDD82 pKa = 3.81NYY84 pKa = 10.71LSAIADD90 pKa = 3.33QMVNSGQGLGIADD103 pKa = 4.03KK104 pKa = 10.68LYY106 pKa = 11.02EE107 pKa = 4.19SMVNNKK113 pKa = 7.46VV114 pKa = 2.94

Molecular weight: 12.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7C7Y0|R7C7Y0_9CLOT Non-specific serine/threonine protein kinase OS=Clostridium sp. CAG:62 OX=1262828 GN=BN737_00008 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 8.56MTYY5 pKa = 8.56QPKK8 pKa = 9.32KK9 pKa = 7.54RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.07VHH16 pKa = 5.95GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTSNGRR28 pKa = 11.84KK29 pKa = 8.91VLAKK33 pKa = 10.18RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.76KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2452

0

2452

800385

29

2177

326.4

36.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.098 ± 0.047

1.517 ± 0.023

5.623 ± 0.043

7.446 ± 0.06

4.044 ± 0.04

6.86 ± 0.044

1.685 ± 0.021

7.045 ± 0.041

8.215 ± 0.059

8.497 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.088 ± 0.023

4.503 ± 0.037

3.137 ± 0.028

3.178 ± 0.03

3.999 ± 0.034

5.994 ± 0.041

5.646 ± 0.048

7.235 ± 0.039

0.885 ± 0.018

4.306 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski