Pseudomonas oryzihabitans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; Pseudomonas putida group

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4281 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D7FBV7|A0A0D7FBV7_9PSED ABC transporter ATP-binding protein OS=Pseudomonas oryzihabitans OX=47885 GN=A4V15_09495 PE=4 SV=1
MM1 pKa = 7.83RR2 pKa = 11.84KK3 pKa = 7.57WQCIVCGYY11 pKa = 10.3IYY13 pKa = 10.54DD14 pKa = 4.33EE15 pKa = 4.4ALGWPDD21 pKa = 5.12DD22 pKa = 4.61GIAPGTRR29 pKa = 11.84WEE31 pKa = 4.75DD32 pKa = 3.57VPEE35 pKa = 4.63DD36 pKa = 4.22WLCPDD41 pKa = 4.21CGVGKK46 pKa = 10.88GDD48 pKa = 4.32FEE50 pKa = 4.8MIAIGG55 pKa = 4.11

Molecular weight: 6.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U4WFK4|A0A0U4WFK4_9PSED Energy transducer TonB OS=Pseudomonas oryzihabitans OX=47885 GN=APT59_02565 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTLKK11 pKa = 10.31RR12 pKa = 11.84ARR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.29NGRR28 pKa = 11.84QVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.98GRR39 pKa = 11.84KK40 pKa = 8.8RR41 pKa = 11.84LAII44 pKa = 4.0

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4281

0

4281

1409937

38

5332

329.3

35.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.015 ± 0.043

0.953 ± 0.01

5.339 ± 0.027

5.791 ± 0.031

3.462 ± 0.022

8.314 ± 0.032

2.17 ± 0.016

4.267 ± 0.028

2.744 ± 0.034

12.626 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.984 ± 0.018

2.47 ± 0.02

5.102 ± 0.033

4.573 ± 0.029

7.281 ± 0.039

5.104 ± 0.024

4.888 ± 0.022

7.086 ± 0.03

1.434 ± 0.016

2.395 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski