Cytobacillus kochii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae;

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4424 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A248TEE4|A0A248TEE4_9BACI UTP--glucose-1-phosphate uridylyltransferase OS=Cytobacillus kochii OX=859143 GN=CKF48_03640 PE=3 SV=1
MM1 pKa = 7.38KK2 pKa = 10.34KK3 pKa = 10.13IIIFLAVIIILFGGVALLTKK23 pKa = 8.94MQNDD27 pKa = 3.6QKK29 pKa = 11.5VSGDD33 pKa = 3.52NPYY36 pKa = 9.32GTTDD40 pKa = 4.34LEE42 pKa = 4.6QEE44 pKa = 4.77TIDD47 pKa = 5.13QLDD50 pKa = 3.93DD51 pKa = 4.1PNYY54 pKa = 10.56SNIVLPEE61 pKa = 4.32DD62 pKa = 4.31LEE64 pKa = 4.58SQLSEE69 pKa = 4.09SGEE72 pKa = 3.8ATVYY76 pKa = 10.02FFSPTCPHH84 pKa = 6.62CQRR87 pKa = 11.84TTPIVAPLSDD97 pKa = 4.4DD98 pKa = 4.09LGVDD102 pKa = 3.88LLQYY106 pKa = 11.31NLLEE110 pKa = 5.4FDD112 pKa = 4.16QGWNDD117 pKa = 3.4FGISEE122 pKa = 4.59TPTIVHH128 pKa = 5.96YY129 pKa = 10.94EE130 pKa = 3.99DD131 pKa = 3.6GKK133 pKa = 10.28EE134 pKa = 3.87VARR137 pKa = 11.84ITGYY141 pKa = 9.08QEE143 pKa = 3.51EE144 pKa = 5.32AVFQDD149 pKa = 3.11WFEE152 pKa = 4.64EE153 pKa = 4.34NVTEE157 pKa = 4.7

Molecular weight: 17.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A248TJ46|A0A248TJ46_9BACI Polyisoprenyl-teichoic acid--peptidoglycan teichoic acid transferase TagU OS=Cytobacillus kochii OX=859143 GN=tagU PE=3 SV=1
MM1 pKa = 7.42RR2 pKa = 11.84VKK4 pKa = 10.51HH5 pKa = 5.97GRR7 pKa = 11.84LTSAIARR14 pKa = 11.84KK15 pKa = 9.67ARR17 pKa = 11.84ICAKK21 pKa = 10.4VKK23 pKa = 10.24GSLRR27 pKa = 11.84RR28 pKa = 11.84IARR31 pKa = 11.84KK32 pKa = 9.65ARR34 pKa = 11.84IRR36 pKa = 11.84AKK38 pKa = 10.92AEE40 pKa = 3.83GSPSRR45 pKa = 11.84IASKK49 pKa = 9.58TKK51 pKa = 9.83IRR53 pKa = 11.84AKK55 pKa = 10.72VKK57 pKa = 10.38GSLSRR62 pKa = 11.84IARR65 pKa = 11.84KK66 pKa = 7.81TKK68 pKa = 9.8IRR70 pKa = 11.84AKK72 pKa = 10.87AEE74 pKa = 4.06GSLSRR79 pKa = 11.84IARR82 pKa = 11.84KK83 pKa = 7.81TKK85 pKa = 9.82IRR87 pKa = 11.84AKK89 pKa = 10.63AKK91 pKa = 9.56GSLRR95 pKa = 11.84RR96 pKa = 11.84IARR99 pKa = 11.84KK100 pKa = 9.74AKK102 pKa = 9.64IRR104 pKa = 11.84AKK106 pKa = 10.69AKK108 pKa = 9.61GSLRR112 pKa = 11.84RR113 pKa = 11.84IARR116 pKa = 11.84KK117 pKa = 9.74AKK119 pKa = 9.64IRR121 pKa = 11.84AKK123 pKa = 10.69AKK125 pKa = 9.61GSLRR129 pKa = 11.84RR130 pKa = 11.84IARR133 pKa = 11.84KK134 pKa = 9.74AKK136 pKa = 9.6IRR138 pKa = 11.84AKK140 pKa = 10.86AEE142 pKa = 3.71GSPRR146 pKa = 11.84RR147 pKa = 11.84IARR150 pKa = 11.84KK151 pKa = 7.36TKK153 pKa = 9.76IRR155 pKa = 11.84AKK157 pKa = 10.63AKK159 pKa = 9.56GSLRR163 pKa = 11.84RR164 pKa = 11.84IARR167 pKa = 11.84KK168 pKa = 9.65ARR170 pKa = 11.84IRR172 pKa = 11.84AKK174 pKa = 10.76AKK176 pKa = 10.06GSPSRR181 pKa = 11.84IARR184 pKa = 11.84KK185 pKa = 7.34TKK187 pKa = 9.82IRR189 pKa = 11.84AIHH192 pKa = 4.83QQ193 pKa = 3.55

Molecular weight: 21.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4424

0

4424

1257253

21

2714

284.2

31.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.952 ± 0.044

0.727 ± 0.01

4.952 ± 0.03

7.643 ± 0.047

4.474 ± 0.03

6.782 ± 0.047

2.197 ± 0.02

8.22 ± 0.035

6.893 ± 0.038

9.81 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.839 ± 0.017

4.436 ± 0.027

3.462 ± 0.023

3.929 ± 0.03

3.876 ± 0.028

6.077 ± 0.029

5.415 ± 0.037

6.781 ± 0.028

0.994 ± 0.013

3.542 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski