Lactobacillus phage EV3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M9JJ52|A0A0M9JJ52_9CAUD Phage tail protein OS=Lactobacillus phage EV3 OX=980881 GN=EV3_005 PE=4 SV=1
MM1 pKa = 7.42IKK3 pKa = 10.05NRR5 pKa = 11.84KK6 pKa = 8.91NYY8 pKa = 8.74STDD11 pKa = 3.83PIEE14 pKa = 3.83PLFYY18 pKa = 10.51IFLGQDD24 pKa = 3.08LNEE27 pKa = 5.13DD28 pKa = 3.78EE29 pKa = 5.36NCFLNYY35 pKa = 7.76TNKK38 pKa = 9.49MNGYY42 pKa = 8.75YY43 pKa = 9.95VDD45 pKa = 4.69SNAEE49 pKa = 3.9SDD51 pKa = 3.7STQTKK56 pKa = 7.78FTYY59 pKa = 10.5DD60 pKa = 3.07EE61 pKa = 4.12FQQAKK66 pKa = 10.19KK67 pKa = 10.5DD68 pKa = 4.1LSNLWFGDD76 pKa = 3.65EE77 pKa = 4.72DD78 pKa = 3.99DD79 pKa = 4.78CLIPVGEE86 pKa = 4.49IDD88 pKa = 5.27DD89 pKa = 4.49LKK91 pKa = 11.33NEE93 pKa = 3.98NKK95 pKa = 9.33ATIGFVNDD103 pKa = 3.56DD104 pKa = 3.92GKK106 pKa = 11.1FEE108 pKa = 4.47KK109 pKa = 10.75LLEE112 pKa = 3.98VDD114 pKa = 5.03KK115 pKa = 10.86FLKK118 pKa = 10.59DD119 pKa = 3.27EE120 pKa = 4.54PEE122 pKa = 3.99GMRR125 pKa = 11.84AMKK128 pKa = 10.42DD129 pKa = 3.43VMSDD133 pKa = 2.89NDD135 pKa = 3.44

Molecular weight: 15.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M7RDN7|A0A0M7RDN7_9CAUD VRR-NUC domain protein OS=Lactobacillus phage EV3 OX=980881 GN=EV3_038 PE=4 SV=1
MM1 pKa = 7.08TAKK4 pKa = 10.32KK5 pKa = 10.33DD6 pKa = 3.49LTGKK10 pKa = 10.39RR11 pKa = 11.84FGRR14 pKa = 11.84LVAICDD20 pKa = 3.21SGKK23 pKa = 10.05RR24 pKa = 11.84NRR26 pKa = 11.84SNVVWKK32 pKa = 9.52CKK34 pKa = 9.92CDD36 pKa = 3.44CGNYY40 pKa = 9.96CYY42 pKa = 10.32ISGSILGRR50 pKa = 11.84HH51 pKa = 5.48TNSCGCLRR59 pKa = 11.84KK60 pKa = 9.54EE61 pKa = 3.66RR62 pKa = 11.84LKK64 pKa = 11.08RR65 pKa = 11.84FVAKK69 pKa = 10.38HH70 pKa = 5.8PEE72 pKa = 4.38LIKK75 pKa = 10.78KK76 pKa = 9.54SNKK79 pKa = 7.07KK80 pKa = 5.66THH82 pKa = 6.68EE83 pKa = 3.64ISHH86 pKa = 6.04FKK88 pKa = 9.86RR89 pKa = 11.84RR90 pKa = 11.84KK91 pKa = 7.65INKK94 pKa = 7.89SGIVGVHH101 pKa = 5.8FDD103 pKa = 3.15KK104 pKa = 11.37SRR106 pKa = 11.84NKK108 pKa = 8.9WVADD112 pKa = 3.5LEE114 pKa = 4.51NNKK117 pKa = 10.1KK118 pKa = 10.56LFLKK122 pKa = 10.54KK123 pKa = 10.11RR124 pKa = 11.84FKK126 pKa = 10.97NKK128 pKa = 9.17QDD130 pKa = 4.05AINARR135 pKa = 11.84LEE137 pKa = 4.21TEE139 pKa = 4.22RR140 pKa = 11.84KK141 pKa = 9.55YY142 pKa = 11.07LPQSQWQHH150 pKa = 4.51VVSQNN155 pKa = 2.92

Molecular weight: 18.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

10793

51

1263

251.0

28.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.3 ± 0.644

0.612 ± 0.147

7.069 ± 0.43

5.114 ± 0.306

4.225 ± 0.314

6.31 ± 0.459

1.899 ± 0.17

6.384 ± 0.277

8.709 ± 0.44

7.829 ± 0.275

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.39 ± 0.151

7.468 ± 0.264

3.465 ± 0.298

4.503 ± 0.312

3.641 ± 0.287

6.736 ± 0.27

6.032 ± 0.308

6.087 ± 0.215

1.482 ± 0.111

3.743 ± 0.318
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski