Halovirus HSTV-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Haloferacalesvirus; unclassified Haloferacalesvirus

Average proteome isoelectric point is 4.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 103 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L7TGL2|L7TGL2_9CAUD Uncharacterized protein OS=Halovirus HSTV-2 OX=1262527 GN=40 PE=4 SV=1
MM1 pKa = 7.1VNYY4 pKa = 10.18GAGLSGDD11 pKa = 3.63KK12 pKa = 10.65VVSTNEE18 pKa = 4.0TADD21 pKa = 4.34DD22 pKa = 5.59PILQYY27 pKa = 11.76DD28 pKa = 3.84NLTIEE33 pKa = 4.93DD34 pKa = 3.93GANFSVASPCILMVRR49 pKa = 11.84DD50 pKa = 3.64TLTVNGILEE59 pKa = 4.64VIDD62 pKa = 4.58GPVGGAGGNGTGYY75 pKa = 10.99GGGGGDD81 pKa = 3.45AGGSILIASKK91 pKa = 10.24HH92 pKa = 4.29VRR94 pKa = 11.84GTGTIVCDD102 pKa = 4.41GKK104 pKa = 10.82DD105 pKa = 3.54GGNGGATNNSNPGGNNGANGVAYY128 pKa = 10.16SINGQIQGAVPQGGNSGRR146 pKa = 11.84SNNNNNQSGNGGNSVNGLSEE166 pKa = 4.5DD167 pKa = 3.58LHH169 pKa = 6.86LKK171 pKa = 10.54SLIEE175 pKa = 5.08DD176 pKa = 3.83YY177 pKa = 10.61LISGAYY183 pKa = 8.85ISEE186 pKa = 4.44SIYY189 pKa = 10.71DD190 pKa = 3.9IISAGSGAGGASGQTEE206 pKa = 4.64SNNGGSASAGGGGAGGSLLSKK227 pKa = 10.4SGSAGDD233 pKa = 3.71GVQVNNNPSSGGGGGGGGAGGFNAMITEE261 pKa = 5.24DD262 pKa = 3.86YY263 pKa = 10.53QGNITVNANGGDD275 pKa = 3.83GGDD278 pKa = 3.48GGTPNDD284 pKa = 3.89ANLAGGGGGGGSGGFGIYY302 pKa = 8.04YY303 pKa = 9.5TNEE306 pKa = 3.56KK307 pKa = 8.91TVPDD311 pKa = 3.89VNVDD315 pKa = 3.32GGLGGSSNAANQSGTDD331 pKa = 3.62GKK333 pKa = 10.73SGSVLVYY340 pKa = 10.42DD341 pKa = 4.44INQLII346 pKa = 3.8

Molecular weight: 32.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L7TGP6|L7TGP6_9CAUD Uncharacterized protein OS=Halovirus HSTV-2 OX=1262527 GN=87 PE=4 SV=1
MM1 pKa = 7.43FLPRR5 pKa = 11.84RR6 pKa = 11.84ISQSLSCLSPSCYY19 pKa = 10.0SRR21 pKa = 11.84PRR23 pKa = 11.84TDD25 pKa = 3.05TKK27 pKa = 11.45ASTTCCLTPSLIGSNPTICFRR48 pKa = 11.84GFPPRR53 pKa = 11.84LPLMLSPSSFAHH65 pKa = 6.05TDD67 pKa = 2.67AGARR71 pKa = 11.84KK72 pKa = 9.0HH73 pKa = 5.43STSAVRR79 pKa = 11.84LRR81 pKa = 11.84RR82 pKa = 11.84SASAAYY88 pKa = 8.41STAEE92 pKa = 4.39RR93 pKa = 11.84IDD95 pKa = 3.67SARR98 pKa = 11.84LNSS101 pKa = 3.56

Molecular weight: 10.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

103

0

103

20552

20

932

199.5

22.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.257 ± 0.265

0.662 ± 0.097

9.002 ± 0.222

9.712 ± 0.31

3.567 ± 0.163

7.897 ± 0.4

2.263 ± 0.152

4.423 ± 0.154

3.576 ± 0.248

7.007 ± 0.202

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.438 ± 0.133

3.961 ± 0.207

4.754 ± 0.16

3.061 ± 0.11

6.126 ± 0.256

5.858 ± 0.185

5.737 ± 0.242

7.104 ± 0.206

1.372 ± 0.1

3.226 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski