Methylosinus sp. R-45379

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylocystaceae; Methylosinus; unclassified Methylosinus

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4368 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177PH06|A0A177PH06_9RHIZ Peptidase M4 OS=Methylosinus sp. R-45379 OX=980563 GN=A1351_11275 PE=4 SV=1
MM1 pKa = 6.85TASALFNTSVMGMSAQTTALSAVAEE26 pKa = 4.52NISNSNTVGYY36 pKa = 10.47KK37 pKa = 9.91EE38 pKa = 4.5ATTQFQTLLTSYY50 pKa = 10.52QGGNNADD57 pKa = 3.83GGVSASNVIEE67 pKa = 4.23VTKK70 pKa = 10.79SGSTLTTTSATDD82 pKa = 3.5LAIQGDD88 pKa = 4.4GFFVVSDD95 pKa = 3.79AAGNTYY101 pKa = 8.57LTRR104 pKa = 11.84AGSFLPDD111 pKa = 3.23SQGRR115 pKa = 11.84LVNTAGYY122 pKa = 10.41YY123 pKa = 10.57LMGYY127 pKa = 9.42SSSAASAPTDD137 pKa = 3.61PSEE140 pKa = 4.47LSTVTIPYY148 pKa = 9.84GKK150 pKa = 10.26EE151 pKa = 2.94IGTASTEE158 pKa = 4.15GSLTANLPSTADD170 pKa = 3.39VIAAADD176 pKa = 3.92LPSANSSTSTYY187 pKa = 9.07TDD189 pKa = 3.17KK190 pKa = 11.41TSVTAYY196 pKa = 10.47DD197 pKa = 3.65NLGNAVTLDD206 pKa = 3.55VYY208 pKa = 10.7FSKK211 pKa = 9.94TSSNNWEE218 pKa = 3.81MSVYY222 pKa = 10.24NAADD226 pKa = 3.5ATSGGFPYY234 pKa = 10.61SSGPLTTQSLTFSATDD250 pKa = 3.79GSISSGSSASIAVPDD265 pKa = 4.48GSTLALDD272 pKa = 4.68LSGLTQLGSSFSVSNPTINGNAASQVSSVSIAKK305 pKa = 10.32DD306 pKa = 3.25GTLSYY311 pKa = 11.55VLGNGQSVSAYY322 pKa = 9.2KK323 pKa = 10.38IPLGTVASPSNLQALSGNTFAVTKK347 pKa = 10.01EE348 pKa = 3.73SGAAYY353 pKa = 10.42LGDD356 pKa = 4.2ANSGSMGAIASDD368 pKa = 3.51QLEE371 pKa = 4.48GSTVDD376 pKa = 5.38LATQLSNMIVAQRR389 pKa = 11.84SFSANSQVFQVASEE403 pKa = 4.21VMQVLNNLKK412 pKa = 10.44

Molecular weight: 41.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177NXJ4|A0A177NXJ4_9RHIZ DDE_Tnp_1 domain-containing protein OS=Methylosinus sp. R-45379 OX=980563 GN=A1351_20440 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84NVLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4368

0

4368

1357770

27

4146

310.8

33.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.69 ± 0.065

0.85 ± 0.012

5.555 ± 0.025

6.108 ± 0.041

3.939 ± 0.022

8.212 ± 0.04

1.95 ± 0.018

4.906 ± 0.024

3.246 ± 0.031

10.323 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.964 ± 0.017

2.381 ± 0.029

5.187 ± 0.032

2.852 ± 0.024

7.803 ± 0.045

5.704 ± 0.036

4.905 ± 0.043

6.951 ± 0.026

1.232 ± 0.015

2.241 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski