Ascodesmis nigricans

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; Pezizomycetes; Pezizales; Ascodesmidaceae; Ascodesmis

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9617 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S2N726|A0A4S2N726_9PEZI HIT-type domain-containing protein OS=Ascodesmis nigricans OX=341454 GN=EX30DRAFT_14869 PE=4 SV=1
MM1 pKa = 7.58SGQGDD6 pKa = 4.08YY7 pKa = 11.06RR8 pKa = 11.84SDD10 pKa = 3.96DD11 pKa = 4.1SEE13 pKa = 6.4DD14 pKa = 3.47SDD16 pKa = 5.29NSLDD20 pKa = 3.51QDD22 pKa = 3.79NYY24 pKa = 8.4STSGSGSDD32 pKa = 3.9SNSDD36 pKa = 3.63DD37 pKa = 3.92DD38 pKa = 4.58VSGGGDD44 pKa = 3.52EE45 pKa = 4.62EE46 pKa = 5.23RR47 pKa = 11.84WTADD51 pKa = 3.2EE52 pKa = 5.76DD53 pKa = 4.38DD54 pKa = 5.19DD55 pKa = 5.35KK56 pKa = 11.77EE57 pKa = 4.23AMKK60 pKa = 10.8LCIHH64 pKa = 7.04CDD66 pKa = 3.03QDD68 pKa = 4.01EE69 pKa = 4.38EE70 pKa = 4.7HH71 pKa = 7.45DD72 pKa = 3.84PATDD76 pKa = 3.51YY77 pKa = 11.42EE78 pKa = 4.6EE79 pKa = 4.26PLEE82 pKa = 4.84CIVCGAFSHH91 pKa = 6.04RR92 pKa = 11.84QCDD95 pKa = 3.13RR96 pKa = 11.84TANDD100 pKa = 3.01NGAVKK105 pKa = 10.22SAHH108 pKa = 6.31NDD110 pKa = 3.21GGG112 pKa = 3.7

Molecular weight: 12.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S2MKV2|A0A4S2MKV2_9PEZI Uncharacterized protein OS=Ascodesmis nigricans OX=341454 GN=EX30DRAFT_344010 PE=4 SV=1
MM1 pKa = 7.15LSSRR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84HH8 pKa = 5.0VAPASTTTHH17 pKa = 4.79TTPRR21 pKa = 11.84RR22 pKa = 11.84SPTTARR28 pKa = 11.84SGGLLSRR35 pKa = 11.84ITGSRR40 pKa = 11.84PPRR43 pKa = 11.84STTTTRR49 pKa = 11.84STRR52 pKa = 11.84TRR54 pKa = 11.84APVHH58 pKa = 4.16HH59 pKa = 7.34HH60 pKa = 5.9KK61 pKa = 10.82RR62 pKa = 11.84KK63 pKa = 6.27TTLGDD68 pKa = 3.57KK69 pKa = 10.98VSGALMRR76 pKa = 11.84LRR78 pKa = 11.84GSLTRR83 pKa = 11.84RR84 pKa = 11.84PGKK87 pKa = 9.96KK88 pKa = 9.44AAGTRR93 pKa = 11.84RR94 pKa = 11.84MRR96 pKa = 11.84GTDD99 pKa = 3.09GRR101 pKa = 11.84GSRR104 pKa = 11.84GVRR107 pKa = 11.84TTRR110 pKa = 11.84ATGGISTTGRR120 pKa = 11.84RR121 pKa = 11.84GRR123 pKa = 11.84RR124 pKa = 11.84GGRR127 pKa = 11.84YY128 pKa = 8.81

Molecular weight: 13.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9617

0

9617

4033769

50

5637

419.4

46.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.972 ± 0.024

1.236 ± 0.01

5.314 ± 0.019

6.617 ± 0.027

3.468 ± 0.014

6.818 ± 0.025

2.608 ± 0.014

4.766 ± 0.017

5.146 ± 0.023

8.642 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.231 ± 0.011

3.507 ± 0.014

6.858 ± 0.034

3.694 ± 0.021

6.448 ± 0.02

8.215 ± 0.031

6.343 ± 0.021

6.155 ± 0.021

1.369 ± 0.01

2.59 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski