Escherichia phage G4 (Bacteriophage G4)

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Bullavirinae; Gequatrovirus; Escherichia virus G4

Average proteome isoelectric point is 7.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P03640|VGE_BPG4 Lysis protein OS=Escherichia phage G4 OX=10843 GN=E PE=4 SV=1
MM1 pKa = 7.37SKK3 pKa = 10.67SNEE6 pKa = 4.12SAVAFQTAIASIKK19 pKa = 9.94LIQASSVLDD28 pKa = 3.62LTEE31 pKa = 5.86DD32 pKa = 3.73DD33 pKa = 5.33FDD35 pKa = 4.59FLTRR39 pKa = 11.84DD40 pKa = 3.8RR41 pKa = 11.84VWIATDD47 pKa = 3.49RR48 pKa = 11.84SRR50 pKa = 11.84ARR52 pKa = 11.84RR53 pKa = 11.84AIEE56 pKa = 3.8ACVYY60 pKa = 8.39GTLDD64 pKa = 3.27FVGYY68 pKa = 9.24PRR70 pKa = 11.84FPAPVEE76 pKa = 4.42FISAVIAYY84 pKa = 7.79YY85 pKa = 8.7VHH87 pKa = 6.96PVNIQTACLIMEE99 pKa = 4.57GAEE102 pKa = 3.88FTEE105 pKa = 4.67NIVNGVEE112 pKa = 4.22RR113 pKa = 11.84PVKK116 pKa = 10.42ASEE119 pKa = 4.24LFAFTLLVRR128 pKa = 11.84AGNKK132 pKa = 9.77DD133 pKa = 3.97LIGHH137 pKa = 7.11AEE139 pKa = 4.04TNIRR143 pKa = 11.84EE144 pKa = 4.13QLRR147 pKa = 11.84AQGVMM152 pKa = 3.56

Molecular weight: 16.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P03654|VGK_BPG4 K protein OS=Escherichia phage G4 OX=10843 GN=K PE=1 SV=1
MM1 pKa = 7.44KK2 pKa = 10.41KK3 pKa = 10.2SIRR6 pKa = 11.84RR7 pKa = 11.84SGGKK11 pKa = 9.66SKK13 pKa = 10.5GARR16 pKa = 11.84LWYY19 pKa = 10.65VGGTQYY25 pKa = 11.94

Molecular weight: 2.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

1

11

2369

25

554

215.4

24.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.851 ± 0.999

0.76 ± 0.129

5.445 ± 0.421

4.432 ± 0.539

3.968 ± 0.414

5.825 ± 0.56

2.406 ± 0.332

4.981 ± 0.399

6.501 ± 0.843

8.991 ± 1.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.039 ± 0.257

6.036 ± 0.363

4.348 ± 0.693

4.179 ± 0.653

5.15 ± 0.468

8.147 ± 0.555

6.838 ± 0.323

6.121 ± 0.553

1.393 ± 0.233

3.588 ± 0.42
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski