Saitoella complicata (strain BCRC 22490 / CBS 7301 / JCM 7358 / NBRC 10748 / NRRL Y-17804)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Taphrinomycotina incertae sedis; Saitoella; Saitoella complicata

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7118 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E9N7Z9|A0A0E9N7Z9_SAICN Uncharacterized protein OS=Saitoella complicata (strain BCRC 22490 / CBS 7301 / JCM 7358 / NBRC 10748 / NRRL Y-17804) OX=698492 GN=G7K_0095-t2 PE=4 SV=1
MM1 pKa = 7.37VNGSFYY7 pKa = 10.94GPQPQRR13 pKa = 11.84TAGVGSYY20 pKa = 10.92SPVAVVNMSEE30 pKa = 4.44SEE32 pKa = 4.06PSQALYY38 pKa = 10.56YY39 pKa = 10.59CHH41 pKa = 7.09EE42 pKa = 5.02CQDD45 pKa = 3.06EE46 pKa = 4.28WNAQEE51 pKa = 4.51TDD53 pKa = 2.87VGMPCPRR60 pKa = 11.84CGSDD64 pKa = 3.19FTEE67 pKa = 5.45VIDD70 pKa = 4.55QGADD74 pKa = 3.38PRR76 pKa = 11.84DD77 pKa = 3.76LLHH80 pKa = 8.02DD81 pKa = 5.16DD82 pKa = 4.51GDD84 pKa = 4.12DD85 pKa = 4.26FEE87 pKa = 8.01DD88 pKa = 5.07DD89 pKa = 5.33DD90 pKa = 6.06EE91 pKa = 6.7YY92 pKa = 11.78DD93 pKa = 4.07DD94 pKa = 6.46DD95 pKa = 5.35EE96 pKa = 6.5DD97 pKa = 4.92VDD99 pKa = 4.22GLGFNVAGPGGMPPFMFGNIAGNNTDD125 pKa = 3.49HH126 pKa = 6.72GPNRR130 pKa = 11.84EE131 pKa = 4.02MMGMLQGLLQQFIGGRR147 pKa = 11.84QPPPRR152 pKa = 11.84ADD154 pKa = 3.49SNEE157 pKa = 4.0NGHH160 pKa = 5.26TTSVDD165 pKa = 3.24MPAQPQQAAGANVQFTFGSMAAGLGVAGTGRR196 pKa = 11.84GDD198 pKa = 3.39RR199 pKa = 11.84QEE201 pKa = 4.3NGSGHH206 pKa = 6.1VPVTDD211 pKa = 4.69LSTFLQQAFGALAGGPADD229 pKa = 3.73GGFAFGGGNGQAGGGLGRR247 pKa = 11.84NLGDD251 pKa = 3.16YY252 pKa = 10.2VFSNRR257 pKa = 11.84GFDD260 pKa = 3.53EE261 pKa = 4.43VVSRR265 pKa = 11.84LMEE268 pKa = 3.71QHH270 pKa = 6.16QGSDD274 pKa = 3.67APPPTPEE281 pKa = 3.89YY282 pKa = 10.56VINAIPAFLVDD293 pKa = 3.62EE294 pKa = 5.22NIAKK298 pKa = 10.16SEE300 pKa = 3.95WDD302 pKa = 3.62CAVCKK307 pKa = 10.46DD308 pKa = 3.52DD309 pKa = 3.6WQIGEE314 pKa = 4.11TALRR318 pKa = 11.84LPCSHH323 pKa = 7.02CFHH326 pKa = 7.15DD327 pKa = 4.32QCIKK331 pKa = 10.47PWLKK335 pKa = 10.93VNSTCPVCRR344 pKa = 11.84SSVLGSGTAQRR355 pKa = 11.84SAEE358 pKa = 4.01QAGGSSVEE366 pKa = 4.28GVTVATQASSSTSHH380 pKa = 5.79QMPGSYY386 pKa = 9.14PASASAPDD394 pKa = 3.88ILEE397 pKa = 4.2QDD399 pKa = 4.28DD400 pKa = 4.34LDD402 pKa = 3.85

Molecular weight: 42.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E9NKP6|A0A0E9NKP6_SAICN Sm domain-containing protein OS=Saitoella complicata (strain BCRC 22490 / CBS 7301 / JCM 7358 / NBRC 10748 / NRRL Y-17804) OX=698492 GN=G7K_4115-t1 PE=3 SV=1
MM1 pKa = 7.87RR2 pKa = 11.84GNKK5 pKa = 8.53VRR7 pKa = 11.84LLPALPLSRR16 pKa = 11.84LRR18 pKa = 11.84ILNLKK23 pKa = 7.7TLHH26 pKa = 6.66LSTSFHH32 pKa = 6.69PLSGPSNLNRR42 pKa = 11.84TTPAAVPRR50 pKa = 11.84PWRR53 pKa = 11.84GRR55 pKa = 11.84LHH57 pKa = 6.38SVPLRR62 pKa = 11.84LRR64 pKa = 11.84QPLTHH69 pKa = 7.04RR70 pKa = 4.3

Molecular weight: 7.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7118

0

7118

3658384

66

5171

514.0

56.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.74 ± 0.028

1.267 ± 0.009

5.311 ± 0.018

6.729 ± 0.029

3.529 ± 0.017

6.99 ± 0.026

2.414 ± 0.013

4.62 ± 0.018

5.04 ± 0.022

8.85 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.303 ± 0.009

3.313 ± 0.015

6.015 ± 0.033

3.729 ± 0.019

6.336 ± 0.029

7.896 ± 0.033

6.228 ± 0.024

6.681 ± 0.019

1.338 ± 0.011

2.67 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski