[Clostridium] thermoalcaliphilum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae; Peptostreptococcaceae incertae sedis

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2026 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V4I440|A0A1V4I440_9FIRM Cobyric acid synthase OS=[Clostridium] thermoalcaliphilum OX=29349 GN=cobQ PE=3 SV=1
MM1 pKa = 7.85SIDD4 pKa = 3.7LDD6 pKa = 3.86EE7 pKa = 6.12AISILNSLFIVPKK20 pKa = 9.18EE21 pKa = 4.35TVCFEE26 pKa = 4.88LDD28 pKa = 3.3DD29 pKa = 4.04RR30 pKa = 11.84CIKK33 pKa = 10.42FRR35 pKa = 11.84VQFYY39 pKa = 8.64EE40 pKa = 3.69EE41 pKa = 3.83CTIYY45 pKa = 10.81VDD47 pKa = 4.21EE48 pKa = 4.44VV49 pKa = 3.24

Molecular weight: 5.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V4I4K5|A0A1V4I4K5_9FIRM Serine acetyltransferase OS=[Clostridium] thermoalcaliphilum OX=29349 GN=cysE_1 PE=4 SV=1
MM1 pKa = 7.35ARR3 pKa = 11.84KK4 pKa = 10.09AMVVKK9 pKa = 9.28QQRR12 pKa = 11.84KK13 pKa = 6.56QKK15 pKa = 9.85YY16 pKa = 5.8KK17 pKa = 7.72TRR19 pKa = 11.84EE20 pKa = 3.77YY21 pKa = 10.12TRR23 pKa = 11.84CTICGRR29 pKa = 11.84PHH31 pKa = 5.69SVLRR35 pKa = 11.84KK36 pKa = 9.55FGICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.97LAYY49 pKa = 9.84KK50 pKa = 10.19GQIPGVKK57 pKa = 9.62KK58 pKa = 10.92SSWW61 pKa = 3.1

Molecular weight: 7.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2026

0

2026

607903

35

1735

300.1

33.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.516 ± 0.063

1.058 ± 0.02

5.749 ± 0.044

7.519 ± 0.068

4.066 ± 0.041

6.488 ± 0.059

1.485 ± 0.022

10.427 ± 0.071

8.818 ± 0.067

8.917 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.645 ± 0.026

6.041 ± 0.046

2.91 ± 0.028

2.433 ± 0.025

3.73 ± 0.037

6.309 ± 0.045

4.682 ± 0.033

6.646 ± 0.049

0.586 ± 0.018

3.974 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski