Helicobacter phage Pt21299RU

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Schmidvirus; unclassified Schmidvirus

Average proteome isoelectric point is 7.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5RFH3|A0A1S5RFH3_9CAUD Uncharacterized protein OS=Helicobacter phage Pt21299RU OX=1852672 PE=4 SV=1
MM1 pKa = 7.66GIKK4 pKa = 9.82EE5 pKa = 4.23KK6 pKa = 10.55EE7 pKa = 3.99IEE9 pKa = 4.18LEE11 pKa = 3.99TLKK14 pKa = 11.01RR15 pKa = 11.84EE16 pKa = 3.99IAQAEE21 pKa = 4.06ASLEE25 pKa = 3.89QDD27 pKa = 4.45FIKK30 pKa = 10.94HH31 pKa = 4.8MVEE34 pKa = 3.69KK35 pKa = 10.0TNEE38 pKa = 3.95KK39 pKa = 10.56VEE41 pKa = 4.87DD42 pKa = 3.83LFFSNKK48 pKa = 8.85PEE50 pKa = 4.27FYY52 pKa = 10.1RR53 pKa = 11.84FVFTEE58 pKa = 3.6QNNYY62 pKa = 9.57LRR64 pKa = 11.84EE65 pKa = 4.0KK66 pKa = 10.13LTDD69 pKa = 3.41KK70 pKa = 10.83VGRR73 pKa = 11.84AMDD76 pKa = 4.45LSDD79 pKa = 4.45EE80 pKa = 4.23IQRR83 pKa = 11.84DD84 pKa = 3.65RR85 pKa = 11.84EE86 pKa = 4.0NEE88 pKa = 3.96EE89 pKa = 3.62IEE91 pKa = 4.04KK92 pKa = 10.98DD93 pKa = 3.25KK94 pKa = 11.4EE95 pKa = 4.14AFLKK99 pKa = 10.25KK100 pKa = 10.1HH101 pKa = 6.07PNIDD105 pKa = 3.86LNEE108 pKa = 4.16LLDD111 pKa = 4.19FYY113 pKa = 11.61NEE115 pKa = 4.19EE116 pKa = 3.62IPNRR120 pKa = 11.84FKK122 pKa = 11.17KK123 pKa = 10.58QIDD126 pKa = 3.72KK127 pKa = 11.52LEE129 pKa = 4.31GEE131 pKa = 4.6AFFEE135 pKa = 4.88AVLDD139 pKa = 3.87YY140 pKa = 11.19FNALNAKK147 pKa = 9.23EE148 pKa = 4.2EE149 pKa = 4.37PKK151 pKa = 10.5SEE153 pKa = 3.99AKK155 pKa = 10.67EE156 pKa = 3.95EE157 pKa = 4.42GNNLPKK163 pKa = 10.37EE164 pKa = 4.0ALGNGVSSVGYY175 pKa = 10.57ANNEE179 pKa = 4.25NIMTRR184 pKa = 11.84YY185 pKa = 9.53

Molecular weight: 21.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5RFH2|A0A1S5RFH2_9CAUD Putative histidine kinase OS=Helicobacter phage Pt21299RU OX=1852672 PE=4 SV=1
MM1 pKa = 7.87RR2 pKa = 11.84SWNYY6 pKa = 8.47QHH8 pKa = 7.35DD9 pKa = 4.31NKK11 pKa = 10.62NFKK14 pKa = 10.09PKK16 pKa = 9.74VWTNSMLLEE25 pKa = 4.59NGLLAILEE33 pKa = 4.1ALKK36 pKa = 10.85KK37 pKa = 10.66LEE39 pKa = 4.22NKK41 pKa = 9.47SRR43 pKa = 11.84SVILEE48 pKa = 3.53RR49 pKa = 11.84LIIFFIEE56 pKa = 4.3TQKK59 pKa = 11.09GQSDD63 pKa = 3.45EE64 pKa = 4.45KK65 pKa = 10.22AWKK68 pKa = 9.37RR69 pKa = 11.84SKK71 pKa = 10.35RR72 pKa = 11.84AYY74 pKa = 9.51KK75 pKa = 10.23RR76 pKa = 11.84SLVNKK81 pKa = 8.77AQKK84 pKa = 10.62NKK86 pKa = 10.28LKK88 pKa = 10.49RR89 pKa = 11.84KK90 pKa = 6.89QLEE93 pKa = 4.34RR94 pKa = 11.84IAKK97 pKa = 6.86TKK99 pKa = 10.2KK100 pKa = 9.47KK101 pKa = 10.2KK102 pKa = 9.93QLQSHH107 pKa = 5.94SNCAFSYY114 pKa = 10.0FEE116 pKa = 4.31RR117 pKa = 11.84SS118 pKa = 3.06

Molecular weight: 14.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24

0

24

7395

41

1754

308.1

35.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.329 ± 0.415

0.636 ± 0.222

5.03 ± 0.553

9.006 ± 0.378

4.855 ± 0.328

3.895 ± 0.298

1.298 ± 0.172

6.815 ± 0.248

11.346 ± 0.493

10.642 ± 0.542

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.839 ± 0.238

7.897 ± 0.509

2.61 ± 0.223

4.557 ± 0.557

4.043 ± 0.254

6.545 ± 0.331

4.746 ± 0.343

4.016 ± 0.57

0.46 ± 0.142

3.435 ± 0.241
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski