Rhodosporidium toruloides (strain ATCC 204091 / IIP 30 / MTCC 1151) (Yeast) (Rhodotorula glutinis (strain ATCC 204091))

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Pucciniomycotina; Microbotryomycetes; Sporidiobolales; Sporidiobolaceae; Rhodotorula; Rhodotorula toruloides

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2816 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G0SUL3|G0SUL3_RHOT2 Uncharacterized protein OS=Rhodosporidium toruloides (strain ATCC 204091 / IIP 30 / MTCC 1151) OX=1001064 GN=RTG_00124 PE=4 SV=1
MM1 pKa = 7.52LLYY4 pKa = 9.56ATLAVIFACSGLVSAAPATAAAGGTASSLDD34 pKa = 3.54ASSKK38 pKa = 10.8AFAAAVSYY46 pKa = 11.3QEE48 pKa = 4.03TCYY51 pKa = 10.21LTLFDD56 pKa = 5.39ADD58 pKa = 4.02EE59 pKa = 4.72NGQDD63 pKa = 3.47VYY65 pKa = 11.51INDD68 pKa = 4.47GYY70 pKa = 11.55KK71 pKa = 9.82KK72 pKa = 10.22DD73 pKa = 3.86SSGKK77 pKa = 8.24EE78 pKa = 3.8YY79 pKa = 10.68YY80 pKa = 10.11YY81 pKa = 10.95EE82 pKa = 4.0EE83 pKa = 4.44ACYY86 pKa = 10.3KK87 pKa = 10.26SSKK90 pKa = 10.38VDD92 pKa = 4.26GKK94 pKa = 7.91TQSNKK99 pKa = 9.3VASSSTTDD107 pKa = 3.2NSPFSSLIGTLGVDD121 pKa = 3.7DD122 pKa = 4.68SVSGFLSGLVSGDD135 pKa = 3.5SLGLGGLVRR144 pKa = 11.84FVKK147 pKa = 10.43RR148 pKa = 11.84QFDD151 pKa = 3.78GKK153 pKa = 9.36TIGAILGQPAAEE165 pKa = 4.29PAASSSSSAAAPTPEE180 pKa = 4.8EE181 pKa = 4.84DD182 pKa = 3.54SATPSSPDD190 pKa = 3.3YY191 pKa = 10.92FDD193 pKa = 5.23APSPTPTPIPTTTAAPSPTAVNPLEE218 pKa = 4.17IANGFGGIQSVGNALPGRR236 pKa = 11.84IGAEE240 pKa = 4.37DD241 pKa = 3.32ILGPALSSANADD253 pKa = 3.41PTSAAFGATTTDD265 pKa = 2.8AGVPATQAAPQQSEE279 pKa = 4.48TPDD282 pKa = 3.64AQPSTSAFTPAPGPPGYY299 pKa = 7.79PTAFTPAAGPPSSPSSFTPADD320 pKa = 4.13GPPSASPTVALNPNNVDD337 pKa = 3.12NAAPQATQFQPVSSPPVVQAPASTFTPAAAPSTQDD372 pKa = 3.14VASPATTQQIQPTPSSLVVQTAAPPVNSAPSFFTSASGVVTPTILATSSPVAADD426 pKa = 3.74TPTPAATSAPSSTVVDD442 pKa = 4.71TPATSSTVPLRR453 pKa = 11.84IYY455 pKa = 10.17VAAASPSSSSVDD467 pKa = 3.18AASSTTTPLTLVSLAPTAVSAQSAA491 pKa = 3.6

Molecular weight: 48.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G0T047|G0T047_RHOT2 Serine/arginine repetitive matrix 1 OS=Rhodosporidium toruloides (strain ATCC 204091 / IIP 30 / MTCC 1151) OX=1001064 GN=RTG_02323 PE=4 SV=1
MM1 pKa = 7.65APLALQEE8 pKa = 4.07AHH10 pKa = 7.01KK11 pKa = 10.52SLKK14 pKa = 9.1TALTRR19 pKa = 11.84AHH21 pKa = 6.9GPACPFYY28 pKa = 10.69HH29 pKa = 7.17RR30 pKa = 11.84PQDD33 pKa = 3.51IPVAASLLVGLSALASPRR51 pKa = 11.84VPFRR55 pKa = 11.84TGSSGANRR63 pKa = 11.84LLVVLRR69 pKa = 11.84RR70 pKa = 11.84SAPSAQARR78 pKa = 11.84VQARR82 pKa = 11.84TFATSVRR89 pKa = 11.84TPSLAQGPRR98 pKa = 11.84GFEE101 pKa = 3.97AGLTGALKK109 pKa = 10.68GATGTSGVGRR119 pKa = 11.84AFQFLAGEE127 pKa = 4.32TGRR130 pKa = 11.84GILRR134 pKa = 11.84HH135 pKa = 5.22AKK137 pKa = 10.23ARR139 pKa = 11.84VEE141 pKa = 4.16SAEE144 pKa = 3.78RR145 pKa = 11.84LAANEE150 pKa = 3.98KK151 pKa = 8.76STRR154 pKa = 11.84TPWFWRR160 pKa = 11.84TTLPRR165 pKa = 11.84TGSFASRR172 pKa = 11.84RR173 pKa = 11.84DD174 pKa = 3.4QWTHH178 pKa = 4.93TLHH181 pKa = 7.21ASTSLKK187 pKa = 10.53CDD189 pKa = 4.1FAPSSTSLDD198 pKa = 3.23PSHH201 pKa = 7.22FNDD204 pKa = 3.94SSDD207 pKa = 3.11SRR209 pKa = 11.84SRR211 pKa = 11.84SRR213 pKa = 11.84SHH215 pKa = 6.21HH216 pKa = 6.07HH217 pKa = 3.58QHH219 pKa = 5.6QRR221 pKa = 11.84QSHH224 pKa = 5.95AEE226 pKa = 4.0SEE228 pKa = 4.38FDD230 pKa = 4.43SAHH233 pKa = 5.69QSCEE237 pKa = 4.05GQGGGQAAPVGGCRR251 pKa = 11.84SKK253 pKa = 11.19GPGWGWYY260 pKa = 9.02LALNEE265 pKa = 4.07RR266 pKa = 11.84RR267 pKa = 11.84RR268 pKa = 11.84RR269 pKa = 11.84DD270 pKa = 3.57PQKK273 pKa = 9.9RR274 pKa = 11.84AHH276 pKa = 6.09FRR278 pKa = 11.84QVYY281 pKa = 10.02RR282 pKa = 11.84SMNPDD287 pKa = 3.11LKK289 pKa = 11.11DD290 pKa = 3.11PGMRR294 pKa = 11.84LCGQWEE300 pKa = 3.92AMRR303 pKa = 11.84WWEE306 pKa = 4.52RR307 pKa = 11.84RR308 pKa = 11.84LKK310 pKa = 10.32MEE312 pKa = 4.89GRR314 pKa = 11.84WKK316 pKa = 10.73RR317 pKa = 11.84NGGKK321 pKa = 9.2WSEE324 pKa = 4.83CYY326 pKa = 10.55EE327 pKa = 4.03EE328 pKa = 4.44MIKK331 pKa = 10.53EE332 pKa = 3.84RR333 pKa = 11.84DD334 pKa = 3.39VKK336 pKa = 10.68LRR338 pKa = 11.84RR339 pKa = 11.84LRR341 pKa = 11.84ALMVRR346 pKa = 11.84LRR348 pKa = 11.84AGAPNHH354 pKa = 5.57RR355 pKa = 11.84LRR357 pKa = 11.84RR358 pKa = 11.84RR359 pKa = 11.84MVDD362 pKa = 2.69SATGGRR368 pKa = 11.84YY369 pKa = 9.38GPSGSGPFNYY379 pKa = 9.9EE380 pKa = 3.49AFRR383 pKa = 11.84RR384 pKa = 11.84DD385 pKa = 3.25YY386 pKa = 9.9WRR388 pKa = 11.84SRR390 pKa = 11.84LAPHH394 pKa = 6.55RR395 pKa = 11.84QSGHH399 pKa = 4.74ARR401 pKa = 11.84RR402 pKa = 11.84SGAKK406 pKa = 9.33RR407 pKa = 11.84SASGFQWLALEE418 pKa = 4.1QRR420 pKa = 11.84RR421 pKa = 11.84RR422 pKa = 11.84RR423 pKa = 11.84AKK425 pKa = 10.34VGWAFFTWRR434 pKa = 11.84ACEE437 pKa = 4.19GPADD441 pKa = 3.45ARR443 pKa = 11.84KK444 pKa = 9.06WIRR447 pKa = 11.84RR448 pKa = 11.84DD449 pKa = 3.2GLVQKK454 pKa = 10.59SILGRR459 pKa = 11.84PQLLSAAPLARR470 pKa = 11.84PPSTILFATSAPRR483 pKa = 11.84LASATHH489 pKa = 5.27SAVRR493 pKa = 11.84AFSTSASRR501 pKa = 11.84QIPLPPPSLPLNILFAAPLCPTEE524 pKa = 4.27CALPLLLPLVSILKK538 pKa = 10.52SSAALNVLATITRR551 pKa = 11.84ISLTLLPLSFRR562 pKa = 11.84GKK564 pKa = 9.33ILHH567 pKa = 5.95SLRR570 pKa = 11.84EE571 pKa = 3.98RR572 pKa = 11.84YY573 pKa = 9.62VRR575 pKa = 11.84DD576 pKa = 3.58PTSLASSILGRR587 pKa = 11.84MVLNSGNAAQLAQPSSGFWRR607 pKa = 11.84WNALVGLPLLLATPLVLLALVALASLEE634 pKa = 4.04RR635 pKa = 11.84TPITGRR641 pKa = 11.84WRR643 pKa = 11.84VVMLSPAEE651 pKa = 3.93EE652 pKa = 4.5AEE654 pKa = 4.34LVDD657 pKa = 5.59SILSSADD664 pKa = 2.78KK665 pKa = 11.1SPFVTSPPEE674 pKa = 3.72GTTRR678 pKa = 11.84DD679 pKa = 3.13WVTILRR685 pKa = 11.84KK686 pKa = 9.91VLEE689 pKa = 4.67LPDD692 pKa = 4.31EE693 pKa = 4.81GVSSATGRR701 pKa = 11.84RR702 pKa = 11.84RR703 pKa = 11.84LLGGEE708 pKa = 4.26VLDD711 pKa = 3.89QRR713 pKa = 11.84DD714 pKa = 3.04WRR716 pKa = 11.84VRR718 pKa = 11.84WTQAVLEE725 pKa = 4.36ALEE728 pKa = 4.54KK729 pKa = 10.46GAPAALTASASSSSPSVMPPPPTAFPLEE757 pKa = 4.27PRR759 pKa = 11.84PEE761 pKa = 4.1ALGQGAAGWADD772 pKa = 3.19EE773 pKa = 4.85LVFSKK778 pKa = 10.57PLEE781 pKa = 4.15ARR783 pKa = 11.84HH784 pKa = 6.06ARR786 pKa = 11.84TEE788 pKa = 3.92HH789 pKa = 7.26DD790 pKa = 3.48EE791 pKa = 4.5GGVPLRR797 pKa = 11.84LEE799 pKa = 4.05YY800 pKa = 10.89DD801 pKa = 3.65LLVVDD806 pKa = 5.49RR807 pKa = 11.84KK808 pKa = 10.41DD809 pKa = 3.42ANAFSFGFGPDD820 pKa = 3.35EE821 pKa = 4.42VPSGRR826 pKa = 11.84AAKK829 pKa = 9.48PRR831 pKa = 11.84RR832 pKa = 11.84GVIVVYY838 pKa = 9.88TGFIDD843 pKa = 4.8EE844 pKa = 4.22ILGRR848 pKa = 11.84SGTDD852 pKa = 3.12VPLLSQPTPPSAKK865 pKa = 9.59RR866 pKa = 11.84SLLGFSRR873 pKa = 11.84STSPSTQPAPRR884 pKa = 11.84DD885 pKa = 3.66PIAANLVPPILPTDD899 pKa = 3.79AQTKK903 pKa = 9.68ALAVLLSHH911 pKa = 6.84EE912 pKa = 4.49LAHH915 pKa = 6.83LALSHH920 pKa = 5.68TLEE923 pKa = 5.05SYY925 pKa = 11.37ASTNLLVPHH934 pKa = 7.2LARR937 pKa = 11.84LTTDD941 pKa = 3.11VLRR944 pKa = 11.84TILYY948 pKa = 8.28PVTAILGPFINDD960 pKa = 3.47ALGGALNEE968 pKa = 4.37GARR971 pKa = 11.84GGLGVLGQAVNSCEE985 pKa = 4.07SRR987 pKa = 11.84KK988 pKa = 10.26LEE990 pKa = 4.16SEE992 pKa = 4.01ADD994 pKa = 3.79VVALRR999 pKa = 11.84MLATSGIDD1007 pKa = 3.18PHH1009 pKa = 6.9CALSFWEE1016 pKa = 5.03DD1017 pKa = 3.32RR1018 pKa = 11.84LSSPAHH1024 pKa = 6.33SPDD1027 pKa = 3.54SPTSSTHH1034 pKa = 5.7YY1035 pKa = 10.18PSLSQPNPLRR1045 pKa = 11.84PHH1047 pKa = 5.76STHH1050 pKa = 5.99EE1051 pKa = 4.06QDD1053 pKa = 5.2SKK1055 pKa = 11.94SLDD1058 pKa = 3.32ALLRR1062 pKa = 11.84SHH1064 pKa = 7.81PIDD1067 pKa = 3.38EE1068 pKa = 4.42EE1069 pKa = 3.88RR1070 pKa = 11.84VEE1072 pKa = 4.48RR1073 pKa = 11.84IRR1075 pKa = 11.84HH1076 pKa = 5.3EE1077 pKa = 3.97LRR1079 pKa = 11.84DD1080 pKa = 3.68WEE1082 pKa = 4.34KK1083 pKa = 9.55WWAEE1087 pKa = 4.1MKK1089 pKa = 10.29GHH1091 pKa = 5.82QAAAAA1096 pKa = 3.77

Molecular weight: 120.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2816

0

2816

1800369

29

4364

639.3

69.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.832 ± 0.043

1.008 ± 0.016

5.488 ± 0.027

6.452 ± 0.039

3.29 ± 0.024

6.912 ± 0.04

2.217 ± 0.015

3.348 ± 0.023

4.231 ± 0.033

9.537 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.575 ± 0.012

2.425 ± 0.017

7.394 ± 0.052

3.566 ± 0.026

7.026 ± 0.033

9.429 ± 0.052

5.777 ± 0.024

6.111 ± 0.031

1.265 ± 0.014

2.118 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski