Candidatus Entotheonella factor

Taxonomy: cellular organisms; Bacteria; Nitrospinae/Tectomicrobia group; Candidatus Tectomicrobia; Candidatus Entotheonella

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8429 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W4LAU2|W4LAU2_9BACT Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex OS=Candidatus Entotheonella factor OX=1429438 GN=ETSY1_32265 PE=3 SV=1
MM1 pKa = 7.52LLSLNTIFTWCLLVAWLVKK20 pKa = 10.41PFGFTADD27 pKa = 3.58AQALLPSDD35 pKa = 4.12VQTVDD40 pKa = 2.93ATGDD44 pKa = 3.5TMVNLTLPHH53 pKa = 6.83GFTLSGAVRR62 pKa = 11.84TEE64 pKa = 3.78RR65 pKa = 11.84GDD67 pKa = 3.65PVALGTLTARR77 pKa = 11.84STGGQFTSSLQGGDD91 pKa = 3.46SAYY94 pKa = 10.62HH95 pKa = 5.98IALPGGTYY103 pKa = 10.67DD104 pKa = 4.65LNLSIPFLEE113 pKa = 4.43SDD115 pKa = 3.49EE116 pKa = 4.27TGIPIFVYY124 pKa = 9.31MASDD128 pKa = 3.77VVAGLGIAADD138 pKa = 3.95TTQDD142 pKa = 3.79LVVPDD147 pKa = 4.86LPDD150 pKa = 3.43LVTVTGFVDD159 pKa = 3.74PQDD162 pKa = 3.92ADD164 pKa = 3.92TVPTEE169 pKa = 4.07GALQLVSTDD178 pKa = 3.04GGTFTLALFEE188 pKa = 5.09DD189 pKa = 6.15FYY191 pKa = 10.01TTRR194 pKa = 11.84LPLNTYY200 pKa = 9.35NVSAALTFTEE210 pKa = 5.42AIGTNGPRR218 pKa = 11.84TTAKK222 pKa = 10.66VIVNVDD228 pKa = 3.01AVTVNDD234 pKa = 4.09EE235 pKa = 3.89PTIYY239 pKa = 10.58DD240 pKa = 3.14ITLPATANLSGTVLDD255 pKa = 4.33SAAAPLAPARR265 pKa = 11.84VVATAVDD272 pKa = 3.38ADD274 pKa = 3.86AAVPSDD280 pKa = 3.19IDD282 pKa = 3.77FSCRR286 pKa = 11.84PEE288 pKa = 4.02VTFSLVPMPVSGTAFLYY305 pKa = 10.49RR306 pKa = 11.84EE307 pKa = 4.47PGEE310 pKa = 4.27SSTVGAYY317 pKa = 10.04HH318 pKa = 6.42MPLPIGTYY326 pKa = 9.69HH327 pKa = 7.29LNVTLGLEE335 pKa = 4.4LQPEE339 pKa = 4.43MVPTLVDD346 pKa = 3.64PEE348 pKa = 4.52VSSSSIVHH356 pKa = 6.24IAMPDD361 pKa = 3.27VALTTDD367 pKa = 3.81TAQNLAVPALPPVVMVSGLVTDD389 pKa = 5.15ALGQPVANAAVVAMTEE405 pKa = 3.94ALAGVANAVRR415 pKa = 11.84FLAEE419 pKa = 3.9VQTQEE424 pKa = 4.23DD425 pKa = 3.98GSYY428 pKa = 10.1EE429 pKa = 4.13LPVLSGTSYY438 pKa = 11.05TIMACPPRR446 pKa = 11.84LQQ448 pKa = 3.45

Molecular weight: 46.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W4LII1|W4LII1_9BACT Uncharacterized protein OS=Candidatus Entotheonella factor OX=1429438 GN=ETSY1_21555 PE=4 SV=1
MM1 pKa = 7.89PKK3 pKa = 9.71MKK5 pKa = 9.95TNRR8 pKa = 11.84SAAKK12 pKa = 10.05RR13 pKa = 11.84FRR15 pKa = 11.84LTARR19 pKa = 11.84GKK21 pKa = 9.33VRR23 pKa = 11.84RR24 pKa = 11.84NQAFTRR30 pKa = 11.84HH31 pKa = 5.85ILTKK35 pKa = 9.37KK36 pKa = 6.62TRR38 pKa = 11.84KK39 pKa = 9.46RR40 pKa = 11.84KK41 pKa = 8.59RR42 pKa = 11.84QLRR45 pKa = 11.84STTLVAAADD54 pKa = 3.59APRR57 pKa = 11.84IKK59 pKa = 10.35RR60 pKa = 11.84IISSS64 pKa = 3.44

Molecular weight: 7.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8429

0

8429

2493613

29

4723

295.8

32.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.08 ± 0.028

1.07 ± 0.01

5.707 ± 0.021

6.085 ± 0.028

3.62 ± 0.016

7.591 ± 0.03

2.74 ± 0.014

5.275 ± 0.021

2.854 ± 0.021

10.487 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.573 ± 0.015

2.769 ± 0.018

5.327 ± 0.02

4.668 ± 0.026

6.685 ± 0.025

5.367 ± 0.017

5.535 ± 0.024

7.183 ± 0.023

1.505 ± 0.013

2.878 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski