Candidatus Accumulibacter sp. BA-93

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Betaproteobacteria incertae sedis; Candidatus Accumulibacter; unclassified Candidatus Accumulibacter

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4038 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A011RI31|A0A011RI31_9PROT 50S ribosomal protein L16 OS=Candidatus Accumulibacter sp. BA-93 OX=1454004 GN=rplP PE=3 SV=1
MM1 pKa = 7.8AEE3 pKa = 4.79DD4 pKa = 4.47GSSVLDD10 pKa = 3.83PSGQDD15 pKa = 2.79GMEE18 pKa = 3.99RR19 pKa = 11.84GTYY22 pKa = 7.27TWNPLTSAFSFTTLVDD38 pKa = 3.73TSGQWGLSHH47 pKa = 7.23SGLTSIGISGDD58 pKa = 3.03TMTIAGGVTLNRR70 pKa = 11.84VTSPTSAIVGSWYY83 pKa = 9.38ATAGGQAASTVLITFLSDD101 pKa = 3.11GSYY104 pKa = 10.82MMAEE108 pKa = 4.77DD109 pKa = 4.74GSSILDD115 pKa = 3.46PSGQDD120 pKa = 3.18GMEE123 pKa = 4.2KK124 pKa = 10.9GSFTWNALTNAFSSTTLVDD143 pKa = 4.11TSGEE147 pKa = 3.97WGLSHH152 pKa = 7.31SNIAFAAVSCNTLQLSDD169 pKa = 4.0GGDD172 pKa = 3.19TFTLVRR178 pKa = 11.84VVSDD182 pKa = 3.85PQVCAIPEE190 pKa = 4.11PQTWANLLDD199 pKa = 4.18SVV201 pKa = 4.52

Molecular weight: 20.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A011QN09|A0A011QN09_9PROT Phosphate transporter OS=Candidatus Accumulibacter sp. BA-93 OX=1454004 GN=pitA_1 PE=3 SV=1
MM1 pKa = 7.68LLLKK5 pKa = 9.58WLYY8 pKa = 9.64TLSPDD13 pKa = 2.93WLFFQPINTLGSVLTRR29 pKa = 11.84LPGSPPPAARR39 pKa = 11.84NLRR42 pKa = 11.84GGTTRR47 pKa = 11.84YY48 pKa = 8.19PRR50 pKa = 11.84PAAQKK55 pKa = 9.48PEE57 pKa = 4.16GYY59 pKa = 9.78EE60 pKa = 3.85PSRR63 pKa = 11.84SQCQSRR69 pKa = 11.84RR70 pKa = 11.84RR71 pKa = 11.84DD72 pKa = 3.66APCLVSWLEE81 pKa = 4.05EE82 pKa = 3.89NCDD85 pKa = 3.37RR86 pKa = 11.84GQVIVAFIGEE96 pKa = 4.3HH97 pKa = 5.13GVLRR101 pKa = 11.84GTVASAAAPNRR112 pKa = 11.84RR113 pKa = 11.84EE114 pKa = 4.02RR115 pKa = 11.84PPSAGARR122 pKa = 11.84HH123 pKa = 6.28CAPCTQTGRR132 pKa = 11.84RR133 pKa = 11.84CRR135 pKa = 11.84IAASKK140 pKa = 9.33PKK142 pKa = 9.79RR143 pKa = 11.84VASGRR148 pKa = 11.84CAQSLAKK155 pKa = 9.77PGSEE159 pKa = 3.98GFPTTSGNGHH169 pKa = 6.44GPLSSRR175 pKa = 11.84AHH177 pKa = 5.97YY178 pKa = 10.28LLSHH182 pKa = 5.98TFLRR186 pKa = 11.84GEE188 pKa = 4.51EE189 pKa = 4.05YY190 pKa = 11.03GQDD193 pKa = 3.36SFPRR197 pKa = 11.84SASGSAPPQTSPAMAAAAPSRR218 pKa = 11.84AA219 pKa = 3.49

Molecular weight: 23.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4038

0

4038

1337804

29

4153

331.3

36.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.48 ± 0.056

1.015 ± 0.015

5.442 ± 0.032

5.636 ± 0.041

3.626 ± 0.026

8.116 ± 0.041

2.19 ± 0.019

4.648 ± 0.029

3.159 ± 0.037

11.254 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.191 ± 0.017

2.609 ± 0.029

4.97 ± 0.029

3.803 ± 0.021

7.369 ± 0.042

5.674 ± 0.032

4.749 ± 0.037

7.405 ± 0.032

1.366 ± 0.016

2.291 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski