Brassica oleracea var. oleracea

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica; Brassica oleracea

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58535 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D3BMH6|A0A0D3BMH6_BRAOL RING-type domain-containing protein OS=Brassica oleracea var. oleracea OX=109376 GN=106328028 PE=4 SV=1
MM1 pKa = 7.51VDD3 pKa = 3.54GVSDD7 pKa = 3.81GVIGRR12 pKa = 11.84GTDD15 pKa = 4.57GICGGKK21 pKa = 9.09IDD23 pKa = 4.56GDD25 pKa = 3.71LGEE28 pKa = 5.51KK29 pKa = 9.73IGEE32 pKa = 4.44CTGGVCDD39 pKa = 4.77GGTEE43 pKa = 3.99GRR45 pKa = 11.84TGRR48 pKa = 11.84ISSVGVGVMINGGSWGDD65 pKa = 3.51MVGDD69 pKa = 3.52VWGGVCDD76 pKa = 4.01GVIGGLTDD84 pKa = 3.69GVFGGGTGGVSGGVIGSIDD103 pKa = 3.62GVNGGGVGEE112 pKa = 4.42VCGGVCGGVVGDD124 pKa = 4.69GLFGGNVGGRR134 pKa = 11.84MLGGVCSGGAPGVIGEE150 pKa = 4.28VV151 pKa = 3.3

Molecular weight: 13.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3AKD4|A0A0D3AKD4_BRAOL AB hydrolase-1 domain-containing protein OS=Brassica oleracea var. oleracea OX=109376 PE=4 SV=1
MM1 pKa = 7.11QLGTHH6 pKa = 5.58RR7 pKa = 11.84HH8 pKa = 4.48GTAGLAGSFVMPTMLLQKK26 pKa = 9.93VPPRR30 pKa = 11.84TGVMFLRR37 pKa = 11.84LLWPKK42 pKa = 9.95LWRR45 pKa = 11.84SKK47 pKa = 10.39RR48 pKa = 11.84QSPLQSLHH56 pKa = 5.88MSVAFVSTPTLKK68 pKa = 10.17TSFRR72 pKa = 11.84SS73 pKa = 3.52

Molecular weight: 8.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58535

0

58535

20370949

20

5370

348.0

38.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.394 ± 0.01

1.842 ± 0.005

5.416 ± 0.009

6.716 ± 0.014

4.272 ± 0.008

6.4 ± 0.012

2.341 ± 0.005

5.054 ± 0.007

6.248 ± 0.01

9.401 ± 0.012

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.488 ± 0.004

4.227 ± 0.006

4.971 ± 0.013

3.489 ± 0.008

5.696 ± 0.008

9.02 ± 0.012

5.182 ± 0.006

6.714 ± 0.009

1.318 ± 0.004

2.789 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski