Toros virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Toros phlebovirus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A161D6V4|A0A161D6V4_9VIRU Viral sRNA Nonstructural protein OS=Toros virus OX=1764085 PE=4 SV=1
MM1 pKa = 7.39NSQYY5 pKa = 11.17LFDD8 pKa = 4.25YY9 pKa = 10.4PVVKK13 pKa = 10.4LDD15 pKa = 3.79SRR17 pKa = 11.84VHH19 pKa = 6.16RR20 pKa = 11.84ALSTVNYY27 pKa = 7.98MAFNKK32 pKa = 9.54FHH34 pKa = 6.64YY35 pKa = 9.91CDD37 pKa = 3.14ISTYY41 pKa = 6.86EE42 pKa = 3.75HH43 pKa = 7.22CEE45 pKa = 3.93FPLEE49 pKa = 4.28KK50 pKa = 10.24YY51 pKa = 10.44SRR53 pKa = 11.84GFGRR57 pKa = 11.84RR58 pKa = 11.84GSLSDD63 pKa = 3.84FYY65 pKa = 11.23LYY67 pKa = 10.82KK68 pKa = 10.4EE69 pKa = 5.11LPASWGPACTISSVKK84 pKa = 10.17PIVYY88 pKa = 9.43PFRR91 pKa = 11.84GLANDD96 pKa = 4.58LSQFDD101 pKa = 3.97MQSFSVPGLQNIRR114 pKa = 11.84KK115 pKa = 8.76ALSWPFGFPDD125 pKa = 3.88LEE127 pKa = 4.02IFEE130 pKa = 4.71ICSEE134 pKa = 4.61GYY136 pKa = 10.11KK137 pKa = 10.35RR138 pKa = 11.84GLDD141 pKa = 3.41TRR143 pKa = 11.84NQLMSYY149 pKa = 8.87ILRR152 pKa = 11.84MADD155 pKa = 3.1SKK157 pKa = 11.78YY158 pKa = 10.57LDD160 pKa = 3.48EE161 pKa = 6.44CIVQAHH167 pKa = 6.19KK168 pKa = 10.71KK169 pKa = 9.63ILSEE173 pKa = 4.13CRR175 pKa = 11.84ALGLQDD181 pKa = 3.43EE182 pKa = 4.98HH183 pKa = 7.89FNGYY187 pKa = 10.23DD188 pKa = 3.38LFRR191 pKa = 11.84EE192 pKa = 4.11ISTLVCTRR200 pKa = 11.84LINAEE205 pKa = 4.45PYY207 pKa = 10.61DD208 pKa = 4.03STSSGSGLEE217 pKa = 3.77IKK219 pKa = 10.58CIIRR223 pKa = 11.84SYY225 pKa = 10.42KK226 pKa = 10.04ISDD229 pKa = 3.53PSSCVGVYY237 pKa = 10.59GSGFWEE243 pKa = 4.15PVNEE247 pKa = 4.25PEE249 pKa = 4.15EE250 pKa = 4.2DD251 pKa = 3.68TYY253 pKa = 11.8EE254 pKa = 4.81DD255 pKa = 4.41PDD257 pKa = 4.25SEE259 pKa = 4.47FF260 pKa = 3.9

Molecular weight: 29.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A161D6V4|A0A161D6V4_9VIRU Viral sRNA Nonstructural protein OS=Toros virus OX=1764085 PE=4 SV=1
MM1 pKa = 7.89EE2 pKa = 6.57DD3 pKa = 3.44YY4 pKa = 11.12QKK6 pKa = 10.78IAVEE10 pKa = 4.38FGEE13 pKa = 4.06QAIDD17 pKa = 3.46EE18 pKa = 4.69TVVQEE23 pKa = 3.8WLQNFAYY30 pKa = 10.29QGFDD34 pKa = 2.91ARR36 pKa = 11.84AVIQNLIKK44 pKa = 10.84LGGSSWEE51 pKa = 3.83EE52 pKa = 3.78DD53 pKa = 3.27AKK55 pKa = 11.55KK56 pKa = 10.12MIILALTRR64 pKa = 11.84GNKK67 pKa = 7.62PKK69 pKa = 10.62KK70 pKa = 7.39MQEE73 pKa = 3.72RR74 pKa = 11.84MSPEE78 pKa = 3.43GAKK81 pKa = 9.58EE82 pKa = 4.01VKK84 pKa = 10.63ALVAKK89 pKa = 10.31YY90 pKa = 10.21KK91 pKa = 10.6LVEE94 pKa = 4.38GKK96 pKa = 9.54PGRR99 pKa = 11.84NGLTLTRR106 pKa = 11.84VAAALAGWTVQAIEE120 pKa = 4.28VVEE123 pKa = 4.23NDD125 pKa = 3.58LPVPGSAMDD134 pKa = 4.86RR135 pKa = 11.84ISGQTYY141 pKa = 7.91PRR143 pKa = 11.84QMMHH147 pKa = 6.99PSFAGLIDD155 pKa = 4.38PSLDD159 pKa = 3.45PEE161 pKa = 4.54DD162 pKa = 4.26FNAIVDD168 pKa = 3.57AHH170 pKa = 6.92KK171 pKa = 10.86LFLYY175 pKa = 8.85MFSKK179 pKa = 9.59TINVGLRR186 pKa = 11.84GASKK190 pKa = 10.7RR191 pKa = 11.84DD192 pKa = 3.27IEE194 pKa = 4.43EE195 pKa = 4.39SFSQPMSAAINSSFIDD211 pKa = 3.35NAQRR215 pKa = 11.84RR216 pKa = 11.84SFLTKK221 pKa = 9.89FSIVTSSSRR230 pKa = 11.84ASSVVKK236 pKa = 10.49KK237 pKa = 10.21IAEE240 pKa = 4.22VYY242 pKa = 10.2RR243 pKa = 11.84KK244 pKa = 10.34LEE246 pKa = 3.85

Molecular weight: 27.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3955

246

2090

988.8

111.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.917 ± 0.664

2.528 ± 0.85

5.588 ± 0.325

7.434 ± 0.373

4.399 ± 0.491

6.119 ± 0.032

2.048 ± 0.177

6.068 ± 0.276

6.574 ± 0.369

8.47 ± 0.473

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.363 ± 0.271

4.02 ± 0.19

3.692 ± 0.382

2.958 ± 0.406

5.74 ± 0.513

8.723 ± 0.52

5.386 ± 0.574

6.802 ± 0.334

1.214 ± 0.058

2.958 ± 0.559
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski