Gemmobacter megaterium

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Gemmobacter

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4016 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N7QR91|A0A1N7QR91_9RHOB 3-alpha domain-containing protein OS=Gemmobacter megaterium OX=1086013 GN=SAMN05421774_12123 PE=4 SV=1
MM1 pKa = 7.47SSYY4 pKa = 11.38VLIPISIGLGLVGISAFFWALRR26 pKa = 11.84HH27 pKa = 5.92DD28 pKa = 4.06QYY30 pKa = 12.1DD31 pKa = 4.04DD32 pKa = 4.16LDD34 pKa = 3.98GAAARR39 pKa = 11.84ILIAPDD45 pKa = 3.43TPYY48 pKa = 10.94PPTDD52 pKa = 3.57NGDD55 pKa = 3.93PAWTTGCPLL64 pKa = 3.61

Molecular weight: 6.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N7N9I9|A0A1N7N9I9_9RHOB DNA-binding transcriptional regulator GntR family OS=Gemmobacter megaterium OX=1086013 GN=SAMN05421774_103223 PE=4 SV=1
MM1 pKa = 7.63LSFGLVAVAAGIVARR16 pKa = 11.84FAVSRR21 pKa = 11.84GGGTGRR27 pKa = 11.84GRR29 pKa = 11.84AVRR32 pKa = 11.84VPVRR36 pKa = 11.84VQARR40 pKa = 11.84TPRR43 pKa = 11.84TPVRR47 pKa = 3.61

Molecular weight: 4.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4016

0

4016

1263230

26

4883

314.5

33.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.437 ± 0.053

0.833 ± 0.012

5.862 ± 0.036

5.194 ± 0.036

3.49 ± 0.022

9.093 ± 0.042

2.096 ± 0.018

4.808 ± 0.027

2.51 ± 0.031

10.509 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.794 ± 0.02

2.224 ± 0.02

5.54 ± 0.031

3.167 ± 0.02

7.474 ± 0.038

4.703 ± 0.023

5.352 ± 0.029

7.432 ± 0.031

1.484 ± 0.016

1.997 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski