Eubacterium siraeum CAG:80

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; environmental samples

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2308 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6R3J8|R6R3J8_9FIRM Type IV secretory pathway VirD4 components OS=Eubacterium siraeum CAG:80 OX=1263080 GN=BN788_00046 PE=3 SV=1
MM1 pKa = 7.95RR2 pKa = 11.84KK3 pKa = 7.73TSEE6 pKa = 4.18KK7 pKa = 10.18ILRR10 pKa = 11.84ALLAVSVLATAVLSSGCLDD29 pKa = 3.9DD30 pKa = 5.22EE31 pKa = 4.87EE32 pKa = 5.26EE33 pKa = 4.71ISDD36 pKa = 3.97STASDD41 pKa = 3.18NRR43 pKa = 11.84QTSDD47 pKa = 2.76GGNAEE52 pKa = 4.11SSEE55 pKa = 4.14GSDD58 pKa = 2.89NKK60 pKa = 10.74YY61 pKa = 10.64DD62 pKa = 3.69GSVTGSRR69 pKa = 11.84ASKK72 pKa = 9.15LTFSGSDD79 pKa = 3.62GISIARR85 pKa = 11.84KK86 pKa = 8.07QRR88 pKa = 11.84EE89 pKa = 4.09AEE91 pKa = 4.03KK92 pKa = 10.89PMGEE96 pKa = 5.21DD97 pKa = 3.04GTQTVFVYY105 pKa = 9.57MCGSDD110 pKa = 4.03LEE112 pKa = 4.68SEE114 pKa = 4.38NGLASGDD121 pKa = 3.83IEE123 pKa = 4.42EE124 pKa = 5.18MIAGSQSEE132 pKa = 4.17NVKK135 pKa = 10.24FVIQTGGAGAWADD148 pKa = 3.63TYY150 pKa = 11.3GISAEE155 pKa = 3.95KK156 pKa = 7.26TQRR159 pKa = 11.84YY160 pKa = 7.45VVTGGEE166 pKa = 3.47ISLIEE171 pKa = 4.2EE172 pKa = 4.3KK173 pKa = 10.85EE174 pKa = 4.23SVNMGKK180 pKa = 10.15EE181 pKa = 3.87DD182 pKa = 3.61VLVDD186 pKa = 3.75FLGWGIEE193 pKa = 4.02NYY195 pKa = 10.13AAAKK199 pKa = 8.98MGLIFWNHH207 pKa = 5.31GGGSISGVCFDD218 pKa = 5.1EE219 pKa = 5.85LNEE222 pKa = 4.12NDD224 pKa = 4.08SLSLEE229 pKa = 4.69EE230 pKa = 5.23IDD232 pKa = 3.98TALTSIYY239 pKa = 11.13DD240 pKa = 3.37KK241 pKa = 9.39MTDD244 pKa = 2.76KK245 pKa = 10.84FAFIGFDD252 pKa = 3.37ACLMATVEE260 pKa = 4.37TANMLVPHH268 pKa = 7.5ADD270 pKa = 3.15YY271 pKa = 10.39MFASEE276 pKa = 4.35EE277 pKa = 4.3TEE279 pKa = 4.27PGYY282 pKa = 11.15GWDD285 pKa = 3.57YY286 pKa = 11.37TEE288 pKa = 4.12IAGFMEE294 pKa = 4.95SNPTADD300 pKa = 3.26TAEE303 pKa = 4.28LGKK306 pKa = 8.6TVADD310 pKa = 3.96SFMASCEE317 pKa = 4.11AIGAGGEE324 pKa = 4.18ATLSITDD331 pKa = 3.96LSRR334 pKa = 11.84IDD336 pKa = 4.09EE337 pKa = 4.27LVKK340 pKa = 10.75AVNDD344 pKa = 3.69AAEE347 pKa = 4.13EE348 pKa = 4.11MNDD351 pKa = 3.3ISSDD355 pKa = 3.57PALLANAVRR364 pKa = 11.84SIYY367 pKa = 7.69TVRR370 pKa = 11.84AYY372 pKa = 10.58GSNNDD377 pKa = 3.54TEE379 pKa = 6.07GYY381 pKa = 9.47TNMVDD386 pKa = 3.79LGSMIAATVSGDD398 pKa = 3.34KK399 pKa = 10.49ADD401 pKa = 3.91KK402 pKa = 11.08VMSALEE408 pKa = 3.89KK409 pKa = 10.25AVVYY413 pKa = 10.48NIYY416 pKa = 11.01GEE418 pKa = 4.89DD419 pKa = 3.67KK420 pKa = 10.98DD421 pKa = 4.55GSTGLSTYY429 pKa = 10.71YY430 pKa = 10.09PFGVSGSAEE439 pKa = 3.76LATFGKK445 pKa = 10.43VCVSPYY451 pKa = 11.2YY452 pKa = 8.6MTFVDD457 pKa = 5.74RR458 pKa = 11.84IAYY461 pKa = 7.64GAQNGSTDD469 pKa = 3.46GYY471 pKa = 10.54SGEE474 pKa = 4.4KK475 pKa = 8.62TWLADD480 pKa = 3.55GAVYY484 pKa = 10.06WSDD487 pKa = 3.18GDD489 pKa = 3.85YY490 pKa = 11.37SDD492 pKa = 5.93YY493 pKa = 10.96EE494 pKa = 4.98SNWEE498 pKa = 4.07HH499 pKa = 6.54YY500 pKa = 10.33NNKK503 pKa = 7.51TDD505 pKa = 4.07NMGFCGDD512 pKa = 3.79KK513 pKa = 10.86SSVQIDD519 pKa = 3.68VGPVLDD525 pKa = 5.52DD526 pKa = 3.81EE527 pKa = 4.89GTFYY531 pKa = 11.33FNVTDD536 pKa = 3.65DD537 pKa = 4.51TINNLASVNCSVYY550 pKa = 10.49RR551 pKa = 11.84VDD553 pKa = 4.54EE554 pKa = 4.26EE555 pKa = 4.17TGEE558 pKa = 4.3LIEE561 pKa = 5.1FGVDD565 pKa = 2.93QSTKK569 pKa = 9.92VDD571 pKa = 4.53FSSGLVEE578 pKa = 6.25DD579 pKa = 4.38NFSGQWYY586 pKa = 8.82MIEE589 pKa = 4.21NNLIPMFIVEE599 pKa = 4.56KK600 pKa = 10.44NGNSSVYY607 pKa = 9.58TSPVKK612 pKa = 10.99LNGKK616 pKa = 6.55EE617 pKa = 4.1TNLRR621 pKa = 11.84IAMTQDD627 pKa = 2.43GNAYY631 pKa = 10.25DD632 pKa = 3.77ITALGTWDD640 pKa = 3.44GVNEE644 pKa = 4.16NGEE647 pKa = 4.41SARR650 pKa = 11.84SVVPLKK656 pKa = 10.61EE657 pKa = 3.74GDD659 pKa = 3.79VIVPIFNTYY668 pKa = 10.71DD669 pKa = 3.4SEE671 pKa = 5.09GNFAGKK677 pKa = 10.48AEE679 pKa = 4.27GDD681 pKa = 3.51EE682 pKa = 4.44CTYY685 pKa = 11.24SGDD688 pKa = 3.76NMIEE692 pKa = 4.17FVNLPAGDD700 pKa = 3.69YY701 pKa = 10.2RR702 pKa = 11.84YY703 pKa = 10.97SFVINDD709 pKa = 3.28IYY711 pKa = 11.6GNVCYY716 pKa = 10.03TGFTVFTTDD725 pKa = 3.59DD726 pKa = 3.97DD727 pKa = 5.22GNVFFTPEE735 pKa = 3.56EE736 pKa = 4.0

Molecular weight: 79.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6RFY1|R6RFY1_9FIRM DUF4430 domain-containing protein OS=Eubacterium siraeum CAG:80 OX=1263080 GN=BN788_01428 PE=4 SV=1
MM1 pKa = 6.78NRR3 pKa = 11.84KK4 pKa = 7.4MKK6 pKa = 9.94KK7 pKa = 8.41YY8 pKa = 10.27RR9 pKa = 11.84YY10 pKa = 9.37IISKK14 pKa = 9.28NKK16 pKa = 9.58KK17 pKa = 7.13VQLYY21 pKa = 8.29HH22 pKa = 6.47ICGCGGCRR30 pKa = 11.84CIRR33 pKa = 11.84NFIRR37 pKa = 11.84LHH39 pKa = 5.17IRR41 pKa = 11.84QGEE44 pKa = 4.09QCVICRR50 pKa = 11.84TGAFDD55 pKa = 5.0FGSGKK60 pKa = 9.6CRR62 pKa = 11.84KK63 pKa = 9.76LGGRR67 pKa = 11.84YY68 pKa = 8.94KK69 pKa = 10.82RR70 pKa = 11.84LGKK73 pKa = 10.31

Molecular weight: 8.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2308

0

2308

734533

29

2601

318.3

35.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.81 ± 0.046

1.747 ± 0.024

6.317 ± 0.041

6.694 ± 0.046

4.123 ± 0.037

6.961 ± 0.05

1.475 ± 0.018

7.595 ± 0.047

7.18 ± 0.048

8.211 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.815 ± 0.029

4.844 ± 0.042

3.207 ± 0.03

2.571 ± 0.027

4.029 ± 0.044

6.573 ± 0.052

6.044 ± 0.052

6.841 ± 0.041

0.755 ± 0.016

4.204 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski