Bacillus lehensis G1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus; Alkalihalobacillus lehensis

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4016 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A060M758|A0A060M758_9BACI Glycosyltransferase OS=Bacillus lehensis G1 OX=1246626 GN=BleG1_3372 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.24KK3 pKa = 9.75VFGVGFVSALAITTLAACGSGSDD26 pKa = 4.56SSTGTNGDD34 pKa = 3.5VVEE37 pKa = 5.28LSFSAWGNPAEE48 pKa = 4.05LDD50 pKa = 3.77VYY52 pKa = 11.09NRR54 pKa = 11.84AVDD57 pKa = 4.08AFNEE61 pKa = 4.29MQDD64 pKa = 3.84DD65 pKa = 4.24VQVTLTGLPNDD76 pKa = 4.44NYY78 pKa = 10.55FQTLTTRR85 pKa = 11.84LQGGQAPDD93 pKa = 3.27VFYY96 pKa = 11.45VGAEE100 pKa = 4.13NISTLMSNGTIEE112 pKa = 4.23PLSDD116 pKa = 3.5FLEE119 pKa = 5.08SGDD122 pKa = 4.02SYY124 pKa = 10.28VTEE127 pKa = 4.98DD128 pKa = 4.84EE129 pKa = 4.2FTEE132 pKa = 4.97DD133 pKa = 1.97IWGASRR139 pKa = 11.84NDD141 pKa = 4.18GIVYY145 pKa = 8.9GLPVDD150 pKa = 4.31CNPILMYY157 pKa = 10.51YY158 pKa = 10.42NKK160 pKa = 10.76ALLEE164 pKa = 4.28EE165 pKa = 4.48NNIPSPQEE173 pKa = 3.56LYY175 pKa = 11.21EE176 pKa = 4.26NGEE179 pKa = 4.4WNWDD183 pKa = 3.47TFEE186 pKa = 6.91DD187 pKa = 3.55ITGQLNEE194 pKa = 4.7SGNHH198 pKa = 5.96GYY200 pKa = 7.92MQEE203 pKa = 4.17SGHH206 pKa = 5.99GAMLNWIWTNGGEE219 pKa = 4.67FATEE223 pKa = 3.73DD224 pKa = 4.45AIVGDD229 pKa = 4.06TDD231 pKa = 3.69GKK233 pKa = 8.98TMEE236 pKa = 4.24AFEE239 pKa = 4.32YY240 pKa = 8.64VDD242 pKa = 4.56RR243 pKa = 11.84MIDD246 pKa = 3.38EE247 pKa = 5.1GNFTYY252 pKa = 10.6AGTMPEE258 pKa = 4.21GQGIEE263 pKa = 4.09AMFMSNQVGFVSAGRR278 pKa = 11.84WLTPMFLDD286 pKa = 3.71TSIDD290 pKa = 3.24FDD292 pKa = 4.79YY293 pKa = 11.26IPFPSNTDD301 pKa = 3.43EE302 pKa = 4.7QIEE305 pKa = 4.28TANIATAYY313 pKa = 8.55MAVNSKK319 pKa = 10.44SDD321 pKa = 3.46HH322 pKa = 6.46VEE324 pKa = 3.46EE325 pKa = 4.04AMKK328 pKa = 10.54FISYY332 pKa = 7.59YY333 pKa = 10.19TSTDD337 pKa = 3.32GQEE340 pKa = 3.6VRR342 pKa = 11.84LDD344 pKa = 3.76GEE346 pKa = 4.75GNAVPSVVGIDD357 pKa = 3.83EE358 pKa = 4.15IVLNQDD364 pKa = 3.18RR365 pKa = 11.84PEE367 pKa = 4.52HH368 pKa = 5.43GQYY371 pKa = 11.25LLDD374 pKa = 3.88ARR376 pKa = 11.84SVGRR380 pKa = 11.84VIGLEE385 pKa = 3.98FTSPGLSEE393 pKa = 4.7DD394 pKa = 4.22LEE396 pKa = 4.95DD397 pKa = 4.29IYY399 pKa = 11.34EE400 pKa = 4.25LMMLGDD406 pKa = 4.16IDD408 pKa = 5.54AGDD411 pKa = 4.32ALEE414 pKa = 4.19QATAKK419 pKa = 10.65AKK421 pKa = 10.61AAVEE425 pKa = 4.11

Molecular weight: 46.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A060M7N8|A0A060M7N8_9BACI Oligopeptide ABC transporter permease OS=Bacillus lehensis G1 OX=1246626 GN=BleG1_4024 PE=3 SV=1
MM1 pKa = 7.71GKK3 pKa = 8.0PTFQPNNRR11 pKa = 11.84KK12 pKa = 9.34RR13 pKa = 11.84KK14 pKa = 8.16KK15 pKa = 9.1NHH17 pKa = 4.73GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.25NGRR29 pKa = 11.84KK30 pKa = 8.73VLSRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84QKK38 pKa = 10.43GRR40 pKa = 11.84KK41 pKa = 8.52VLSAA45 pKa = 4.05

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4016

0

4016

1166809

29

3427

290.5

32.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.492 ± 0.047

0.688 ± 0.011

5.153 ± 0.04

7.492 ± 0.045

4.537 ± 0.035

6.886 ± 0.049

2.368 ± 0.022

7.109 ± 0.04

5.766 ± 0.043

10.044 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.728 ± 0.021

3.954 ± 0.032

3.608 ± 0.023

4.138 ± 0.029

4.25 ± 0.028

6.115 ± 0.03

5.943 ± 0.042

7.219 ± 0.039

1.053 ± 0.014

3.456 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski