Beihai rhabdo-like virus 4

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Nyamiviridae; Berhavirus; Beihai berhavirus

Average proteome isoelectric point is 7.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KMN2|A0A1L3KMN2_9MONO Large structural protein OS=Beihai rhabdo-like virus 4 OX=1922654 PE=4 SV=1
MM1 pKa = 7.03LQEE4 pKa = 4.45AGIEE8 pKa = 4.49FILMPTKK15 pKa = 10.56VFASGKK21 pKa = 10.29VNLKK25 pKa = 10.53DD26 pKa = 3.57QMSKK30 pKa = 10.97GLFWEE35 pKa = 4.7DD36 pKa = 4.17LGSLDD41 pKa = 4.56LLPYY45 pKa = 10.2ISNPRR50 pKa = 11.84VFNAVTEE57 pKa = 4.23LPEE60 pKa = 3.97TLKK63 pKa = 11.16YY64 pKa = 9.59CINMAGGINYY74 pKa = 9.55LGWTKK79 pKa = 10.72EE80 pKa = 4.13SAPGDD85 pKa = 3.98TPMSASSISTPAFPKK100 pKa = 8.7QTPAGGQGEE109 pKa = 4.71DD110 pKa = 3.99LLSAIQRR117 pKa = 11.84AAAEE121 pKa = 4.07RR122 pKa = 11.84AAQLQAEE129 pKa = 4.47EE130 pKa = 4.4EE131 pKa = 4.1ARR133 pKa = 11.84SRR135 pKa = 11.84SEE137 pKa = 4.03LRR139 pKa = 11.84QAQDD143 pKa = 2.64QDD145 pKa = 3.27GRR147 pKa = 11.84RR148 pKa = 11.84NPVADD153 pKa = 4.05RR154 pKa = 11.84DD155 pKa = 3.83HH156 pKa = 7.09RR157 pKa = 11.84PAGGGGGPGSSDD169 pKa = 3.24EE170 pKa = 4.22GDD172 pKa = 3.35EE173 pKa = 4.3TGDD176 pKa = 3.53DD177 pKa = 3.66VSSIEE182 pKa = 3.95SDD184 pKa = 3.55QEE186 pKa = 4.02GGEE189 pKa = 3.99VDD191 pKa = 3.58GQGTRR196 pKa = 11.84GGGAGGSTGRR206 pKa = 11.84GGGRR210 pKa = 11.84GDD212 pKa = 3.97NRR214 pKa = 11.84GTGQGEE220 pKa = 4.15GRR222 pKa = 11.84DD223 pKa = 3.53EE224 pKa = 4.03RR225 pKa = 11.84EE226 pKa = 3.52RR227 pKa = 11.84RR228 pKa = 11.84DD229 pKa = 3.27RR230 pKa = 11.84RR231 pKa = 11.84NRR233 pKa = 11.84RR234 pKa = 11.84TGRR237 pKa = 11.84GGRR240 pKa = 11.84DD241 pKa = 2.83RR242 pKa = 11.84RR243 pKa = 11.84GGRR246 pKa = 11.84GGRR249 pKa = 11.84GGGQDD254 pKa = 3.01RR255 pKa = 11.84DD256 pKa = 3.16RR257 pKa = 11.84DD258 pKa = 3.57RR259 pKa = 11.84RR260 pKa = 11.84EE261 pKa = 3.26ISGPQKK267 pKa = 10.64NKK269 pKa = 9.81VPRR272 pKa = 11.84EE273 pKa = 3.93MDD275 pKa = 3.37SAALGHH281 pKa = 6.77EE282 pKa = 4.4YY283 pKa = 10.42PEE285 pKa = 4.23ATLYY289 pKa = 10.2TDD291 pKa = 4.02PDD293 pKa = 3.22RR294 pKa = 11.84AYY296 pKa = 10.85ADD298 pKa = 3.71YY299 pKa = 10.82QRR301 pKa = 11.84EE302 pKa = 3.88ADD304 pKa = 4.16ALKK307 pKa = 9.98VWTPASEE314 pKa = 4.5VPRR317 pKa = 11.84PNPGLALINLLITCAWFTFNSLQIQNVRR345 pKa = 11.84AVRR348 pKa = 11.84SQPLKK353 pKa = 10.55PEE355 pKa = 4.21RR356 pKa = 11.84KK357 pKa = 7.42TDD359 pKa = 2.99AMRR362 pKa = 11.84NDD364 pKa = 3.2PHH366 pKa = 8.38LDD368 pKa = 3.15LRR370 pKa = 11.84YY371 pKa = 9.76LYY373 pKa = 10.56HH374 pKa = 6.96GFLAILCAIKK384 pKa = 10.63PMTEE388 pKa = 4.12DD389 pKa = 2.96NASYY393 pKa = 9.9VWKK396 pKa = 10.29RR397 pKa = 11.84FCAAVNSQDD406 pKa = 5.32LDD408 pKa = 3.54IEE410 pKa = 4.51LKK412 pKa = 10.96NKK414 pKa = 10.53LEE416 pKa = 4.02LVGVSLKK423 pKa = 10.53SLCDD427 pKa = 3.18AAAHH431 pKa = 6.23IKK433 pKa = 10.33RR434 pKa = 11.84YY435 pKa = 9.56IDD437 pKa = 3.81PKK439 pKa = 11.08DD440 pKa = 3.51VLSLLRR446 pKa = 11.84EE447 pKa = 4.14LDD449 pKa = 3.32VDD451 pKa = 4.85DD452 pKa = 5.92PDD454 pKa = 4.63LVAEE458 pKa = 4.7FDD460 pKa = 4.24LKK462 pKa = 10.68PVHH465 pKa = 6.98KK466 pKa = 10.63GLLQQMRR473 pKa = 11.84LIYY476 pKa = 10.54SGVAMARR483 pKa = 11.84IMQMEE488 pKa = 4.82KK489 pKa = 9.35WAQLFDD495 pKa = 4.64CRR497 pKa = 11.84AHH499 pKa = 5.64YY500 pKa = 10.59CNIVVSEE507 pKa = 4.55IISFLSIVKK516 pKa = 10.28ALRR519 pKa = 11.84KK520 pKa = 10.07KK521 pKa = 10.71FGEE524 pKa = 4.2DD525 pKa = 3.49FNFLRR530 pKa = 11.84IDD532 pKa = 3.55RR533 pKa = 11.84PKK535 pKa = 10.4EE536 pKa = 3.85LEE538 pKa = 4.03PLSSRR543 pKa = 11.84KK544 pKa = 9.02FPHH547 pKa = 6.55LAYY550 pKa = 10.44CATEE554 pKa = 3.81SGYY557 pKa = 7.27MTKK560 pKa = 10.53SFTRR564 pKa = 11.84KK565 pKa = 7.8MAKK568 pKa = 9.57SDD570 pKa = 3.75QPLMMPAKK578 pKa = 9.1EE579 pKa = 4.21LKK581 pKa = 10.95VMIKK585 pKa = 10.51DD586 pKa = 3.52RR587 pKa = 11.84LRR589 pKa = 11.84EE590 pKa = 3.92RR591 pKa = 11.84NILSPKK597 pKa = 9.92QILALRR603 pKa = 11.84SLGIALPEE611 pKa = 5.2EE612 pKa = 4.17KK613 pKa = 9.5TQWQGTKK620 pKa = 10.06RR621 pKa = 11.84PRR623 pKa = 11.84EE624 pKa = 4.14DD625 pKa = 3.08QAGPSTKK632 pKa = 9.92RR633 pKa = 11.84PKK635 pKa = 10.09PSNAYY640 pKa = 8.08FTEE643 pKa = 4.33SSEE646 pKa = 4.23EE647 pKa = 4.0EE648 pKa = 4.02SDD650 pKa = 3.56EE651 pKa = 4.77DD652 pKa = 4.32LL653 pKa = 5.24

Molecular weight: 72.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KMQ1|A0A1L3KMQ1_9MONO Uncharacterized protein OS=Beihai rhabdo-like virus 4 OX=1922654 PE=4 SV=1
MM1 pKa = 7.61SGRR4 pKa = 11.84IRR6 pKa = 11.84PRR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84LPQVSSAQAAGGAEE24 pKa = 4.06RR25 pKa = 11.84FRR27 pKa = 11.84CYY29 pKa = 10.52LGVTFRR35 pKa = 11.84FQNGLSLHH43 pKa = 5.29TVKK46 pKa = 11.08SNILAIHH53 pKa = 6.84LKK55 pKa = 9.97RR56 pKa = 11.84PYY58 pKa = 10.23VSRR61 pKa = 11.84GYY63 pKa = 10.01IRR65 pKa = 11.84EE66 pKa = 4.21LKK68 pKa = 9.7LTLQRR73 pKa = 11.84VGGPSRR79 pKa = 11.84IAVRR83 pKa = 11.84QPSGDD88 pKa = 3.57NPQTPTVCHH97 pKa = 5.43VCRR100 pKa = 11.84DD101 pKa = 3.43EE102 pKa = 4.75RR103 pKa = 11.84PIYY106 pKa = 9.63TYY108 pKa = 10.84LWSDD112 pKa = 3.51LGISEE117 pKa = 4.49TWCKK121 pKa = 10.66GDD123 pKa = 3.52EE124 pKa = 4.24APTISVDD131 pKa = 3.14NFKK134 pKa = 10.7VARR137 pKa = 11.84VLEE140 pKa = 4.49DD141 pKa = 4.49GYY143 pKa = 11.58CEE145 pKa = 3.89FLYY148 pKa = 10.71TEE150 pKa = 4.44TCC152 pKa = 3.47

Molecular weight: 17.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2661

152

1856

887.0

100.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.013 ± 1.0

2.067 ± 0.611

6.201 ± 0.806

5.787 ± 0.693

4.247 ± 0.747

6.276 ± 1.429

2.518 ± 0.614

5.637 ± 0.623

6.464 ± 0.977

9.583 ± 0.359

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.818 ± 0.631

3.983 ± 0.544

4.434 ± 0.789

3.457 ± 0.476

7.253 ± 1.446

6.99 ± 0.124

5.224 ± 0.632

6.276 ± 1.089

1.24 ± 0.075

3.533 ± 0.536
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski