Gordonia phage Lysidious

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fairfaxidumvirus; unclassified Fairfaxidumvirus

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D1GG63|A0A2D1GG63_9CAUD Uncharacterized protein OS=Gordonia phage Lysidious OX=2041514 GN=SEA_LYSIDIOUS_24 PE=4 SV=1
MM1 pKa = 7.46ANDD4 pKa = 4.61TIFEE8 pKa = 4.28LPEE11 pKa = 4.65IPGVTFTASYY21 pKa = 10.84GSGGEE26 pKa = 4.12TGLPSNWIRR35 pKa = 11.84IVGTVEE41 pKa = 3.91NPWYY45 pKa = 10.09EE46 pKa = 3.64PTYY49 pKa = 11.14NYY51 pKa = 11.12GLDD54 pKa = 3.95PNGHH58 pKa = 5.7TEE60 pKa = 4.3VTDD63 pKa = 3.08PWKK66 pKa = 10.72RR67 pKa = 11.84HH68 pKa = 4.03TQHH71 pKa = 7.02PKK73 pKa = 7.65VCEE76 pKa = 3.91MGFGGPNNSGLPTEE90 pKa = 5.03PPPPPLPAEE99 pKa = 4.28SEE101 pKa = 4.06PTPDD105 pKa = 4.02IEE107 pKa = 5.1EE108 pKa = 4.49PTDD111 pKa = 3.42GG112 pKa = 4.69

Molecular weight: 12.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1GG93|A0A2D1GG93_9CAUD Uncharacterized protein OS=Gordonia phage Lysidious OX=2041514 GN=SEA_LYSIDIOUS_66 PE=4 SV=1
MM1 pKa = 7.46SEE3 pKa = 3.27QWLPVPGWDD12 pKa = 3.69GFYY15 pKa = 10.63EE16 pKa = 4.27VSDD19 pKa = 3.36QGRR22 pKa = 11.84IRR24 pKa = 11.84SVDD27 pKa = 3.16RR28 pKa = 11.84VIRR31 pKa = 11.84RR32 pKa = 11.84GNGSPQHH39 pKa = 5.82IGPRR43 pKa = 11.84ILAANPRR50 pKa = 11.84PSGHH54 pKa = 6.28LCVRR58 pKa = 11.84LYY60 pKa = 11.0RR61 pKa = 11.84NGRR64 pKa = 11.84GVGMDD69 pKa = 2.8VHH71 pKa = 6.06TAVLTAFVGPRR82 pKa = 11.84PDD84 pKa = 2.95GHH86 pKa = 6.05EE87 pKa = 4.27ACHH90 pKa = 6.51SNGDD94 pKa = 3.56ATDD97 pKa = 3.42NRR99 pKa = 11.84LTNLRR104 pKa = 11.84WDD106 pKa = 3.67TRR108 pKa = 11.84SEE110 pKa = 4.17NNFDD114 pKa = 3.78RR115 pKa = 11.84VRR117 pKa = 11.84HH118 pKa = 4.75GTHH121 pKa = 6.2NNSTKK126 pKa = 8.88TRR128 pKa = 11.84CARR131 pKa = 11.84GHH133 pKa = 6.95LYY135 pKa = 10.83DD136 pKa = 3.88EE137 pKa = 5.23ANTYY141 pKa = 7.61RR142 pKa = 11.84TPSRR146 pKa = 11.84PTARR150 pKa = 11.84YY151 pKa = 8.85CRR153 pKa = 11.84KK154 pKa = 8.45CTAINGRR161 pKa = 11.84NYY163 pKa = 10.86LDD165 pKa = 3.44RR166 pKa = 11.84KK167 pKa = 8.77SNRR170 pKa = 11.84RR171 pKa = 11.84SIPAA175 pKa = 3.66

Molecular weight: 19.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

16494

25

1847

198.7

21.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.35 ± 0.488

0.976 ± 0.148

7.039 ± 0.305

5.626 ± 0.288

2.686 ± 0.15

8.106 ± 0.392

2.213 ± 0.192

4.456 ± 0.17

3.28 ± 0.206

7.585 ± 0.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.152 ± 0.162

3.044 ± 0.145

5.845 ± 0.237

3.383 ± 0.15

7.948 ± 0.452

5.281 ± 0.237

6.778 ± 0.233

7.918 ± 0.239

2.031 ± 0.134

2.304 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski