Pseudomonas phage 67PfluR64PP

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Pifdecavirus; unclassified Pifdecavirus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1PH11|A0A2S1PH11_9CAUD Fe2OG dioxygenase domain-containing protein OS=Pseudomonas phage 67PfluR64PP OX=2163980 PE=4 SV=1
MM1 pKa = 7.55SKK3 pKa = 10.7FSTFNLIAGFNQVLAHH19 pKa = 6.73LNSEE23 pKa = 4.31EE24 pKa = 4.25NEE26 pKa = 4.04LAAQFAEE33 pKa = 4.37TLKK36 pKa = 10.45EE37 pKa = 4.03AQGDD41 pKa = 4.33VYY43 pKa = 11.07HH44 pKa = 7.16LNNLQNRR51 pKa = 11.84GVDD54 pKa = 2.99NWDD57 pKa = 3.5GYY59 pKa = 9.55VGRR62 pKa = 11.84GHH64 pKa = 6.28EE65 pKa = 4.16EE66 pKa = 4.06CEE68 pKa = 3.88ACGFDD73 pKa = 5.53EE74 pKa = 6.18DD75 pKa = 4.54GCEE78 pKa = 4.72CGGDD82 pKa = 3.44QQ83 pKa = 5.17

Molecular weight: 9.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1PGV8|A0A2S1PGV8_9CAUD Tail fiber protein OS=Pseudomonas phage 67PfluR64PP OX=2163980 PE=4 SV=1
MM1 pKa = 7.44SIKK4 pKa = 9.38VTLKK8 pKa = 9.72QLRR11 pKa = 11.84NFDD14 pKa = 3.98LVQALLAAAGRR25 pKa = 11.84LQAKK29 pKa = 9.27RR30 pKa = 11.84VARR33 pKa = 11.84LRR35 pKa = 11.84ARR37 pKa = 11.84EE38 pKa = 3.92AALTATIRR46 pKa = 11.84AASEE50 pKa = 4.05ALVDD54 pKa = 3.84TQKK57 pKa = 11.24ARR59 pKa = 11.84IDD61 pKa = 3.45EE62 pKa = 4.27QYY64 pKa = 10.63RR65 pKa = 11.84DD66 pKa = 3.36LRR68 pKa = 11.84VIVKK72 pKa = 10.01

Molecular weight: 8.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

12409

39

1333

243.3

26.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.888 ± 0.482

0.846 ± 0.14

6.431 ± 0.296

6.737 ± 0.324

3.715 ± 0.178

8.26 ± 0.363

2.353 ± 0.181

4.44 ± 0.159

5.971 ± 0.369

8.478 ± 0.286

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.99 ± 0.244

3.522 ± 0.184

3.755 ± 0.166

4.295 ± 0.285

5.899 ± 0.174

5.569 ± 0.295

5.593 ± 0.201

6.971 ± 0.351

1.386 ± 0.183

2.901 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski