Paenibacillus thalictri

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7735 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q9DMW2|A0A4Q9DMW2_9BACL Glycosyltransferase OS=Paenibacillus thalictri OX=2527873 GN=EYB31_19680 PE=4 SV=1
MM1 pKa = 8.25DD2 pKa = 5.87PNQLQALIFGSDD14 pKa = 3.38GTNALPINTDD24 pKa = 2.55STGRR28 pKa = 11.84INIGAVNTVTAVLGATITAGTISATILGGTIAATVVAGTINAVEE72 pKa = 4.08AATIFGGTINAVEE85 pKa = 4.11AATIFGGTINAVEE98 pKa = 4.11AATIFGGTINAVEE111 pKa = 4.11AATIFGGTINAVEE124 pKa = 4.11AATIFGGTINAVEE137 pKa = 4.11AATIFGGTINAVEE150 pKa = 4.11AATIFGGTINAVEE163 pKa = 4.11AATIFGGTINAVEE176 pKa = 4.11AATIFGGTINAVEE189 pKa = 4.11AATIFGGTINAVEE202 pKa = 4.11AATIFGGTINAVEE215 pKa = 4.11AATIFGGTINAVEE228 pKa = 4.11AATIFGGTINAVEE241 pKa = 4.11AATIFGGTINAVEE254 pKa = 4.11AATIFGGTINAVEE267 pKa = 4.21AATIVGGTLSATILAGTITTISQNNYY293 pKa = 8.46NQFSALGFVVDD304 pKa = 3.94STSFTEE310 pKa = 4.72IPAASLMQNSYY321 pKa = 9.66PYY323 pKa = 10.36KK324 pKa = 8.27VYY326 pKa = 10.88SYY328 pKa = 10.95LVYY331 pKa = 10.98NNTPAVTVNARR342 pKa = 11.84LEE344 pKa = 4.02ISSDD348 pKa = 3.06GSRR351 pKa = 11.84WIADD355 pKa = 3.32QTITGLLTNGVVLTPYY371 pKa = 10.45RR372 pKa = 11.84FAKK375 pKa = 7.72YY376 pKa = 9.02TRR378 pKa = 11.84VTLATAQTDD387 pKa = 3.76TANVDD392 pKa = 3.42VYY394 pKa = 11.6LDD396 pKa = 3.61AQVV399 pKa = 2.97

Molecular weight: 39.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q9DRU7|A0A4Q9DRU7_9BACL Nucleotide pyrophosphatase OS=Paenibacillus thalictri OX=2527873 GN=EYB31_13975 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.87PNVRR10 pKa = 11.84KK11 pKa = 9.84RR12 pKa = 11.84KK13 pKa = 8.89KK14 pKa = 8.64VHH16 pKa = 5.57GFRR19 pKa = 11.84KK20 pKa = 10.0RR21 pKa = 11.84MSTVNGRR28 pKa = 11.84KK29 pKa = 8.5VLKK32 pKa = 10.39ARR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.21GRR39 pKa = 11.84KK40 pKa = 8.56VLSAA44 pKa = 4.05

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7735

0

7735

2731292

19

3290

353.1

39.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.642 ± 0.035

0.813 ± 0.009

5.083 ± 0.02

6.268 ± 0.029

4.179 ± 0.02

7.508 ± 0.03

2.119 ± 0.014

6.484 ± 0.024

5.376 ± 0.027

9.985 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.856 ± 0.013

3.795 ± 0.022

4.231 ± 0.017

3.992 ± 0.019

4.904 ± 0.024

6.324 ± 0.025

5.428 ± 0.03

7.104 ± 0.021

1.328 ± 0.011

3.582 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski