Halocynthia phage JM-2012

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 173 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1VXF6|I1VXF6_9CAUD Uncharacterized protein OS=Halocynthia phage JM-2012 OX=1173297 GN=TSMG0114 PE=4 SV=1
MM1 pKa = 7.86SDD3 pKa = 3.14TQRR6 pKa = 11.84VVVCFEE12 pKa = 3.95EE13 pKa = 7.04DD14 pKa = 2.68IDD16 pKa = 5.4AEE18 pKa = 4.26LFIDD22 pKa = 5.02NITTVMSQFHH32 pKa = 5.61YY33 pKa = 11.17AEE35 pKa = 5.97DD36 pKa = 3.21ILADD40 pKa = 3.93VRR42 pKa = 11.84YY43 pKa = 8.91QDD45 pKa = 4.18CSVEE49 pKa = 4.26VTDD52 pKa = 4.12TAISHH57 pKa = 6.5IEE59 pKa = 4.07RR60 pKa = 11.84ACMLAMEE67 pKa = 4.94YY68 pKa = 11.01YY69 pKa = 9.5PGRR72 pKa = 11.84KK73 pKa = 9.18AFFEE77 pKa = 4.31SVDD80 pKa = 3.6IIDD83 pKa = 3.95NKK85 pKa = 10.02MVAFTIGADD94 pKa = 3.23

Molecular weight: 10.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1VXG1|I1VXG1_9CAUD Uncharacterized protein OS=Halocynthia phage JM-2012 OX=1173297 GN=TSMG0119 PE=4 SV=1
MM1 pKa = 7.44IVFKK5 pKa = 10.81GKK7 pKa = 9.66EE8 pKa = 3.76VSAWTLAKK16 pKa = 10.03RR17 pKa = 11.84YY18 pKa = 9.29NISATTIMRR27 pKa = 11.84RR28 pKa = 11.84WNEE31 pKa = 3.49GLRR34 pKa = 11.84DD35 pKa = 3.46EE36 pKa = 5.52KK37 pKa = 11.07LIKK40 pKa = 10.15KK41 pKa = 8.49PKK43 pKa = 9.86KK44 pKa = 8.54PVKK47 pKa = 10.19VKK49 pKa = 10.66YY50 pKa = 9.99KK51 pKa = 10.63NKK53 pKa = 10.08FYY55 pKa = 10.26TLRR58 pKa = 11.84QLASVTSIPYY68 pKa = 7.84GTLKK72 pKa = 9.76GRR74 pKa = 11.84HH75 pKa = 4.61MSGLRR80 pKa = 11.84GDD82 pKa = 4.15KK83 pKa = 10.52LCNATHH89 pKa = 6.54ARR91 pKa = 11.84TGAIHH96 pKa = 6.69NGPKK100 pKa = 9.0LTPDD104 pKa = 3.29KK105 pKa = 11.68VMDD108 pKa = 4.26IYY110 pKa = 11.91SMAHH114 pKa = 6.14KK115 pKa = 10.24EE116 pKa = 4.14MYY118 pKa = 9.61SQDD121 pKa = 3.5VIADD125 pKa = 3.46IFKK128 pKa = 9.92IDD130 pKa = 3.49QSTVSDD136 pKa = 3.34IKK138 pKa = 10.85LGRR141 pKa = 11.84RR142 pKa = 11.84WSSVTKK148 pKa = 8.99HH149 pKa = 5.36TRR151 pKa = 11.84NTQQ154 pKa = 2.78

Molecular weight: 17.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

173

0

173

51777

38

2278

299.3

33.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.392 ± 0.206

0.892 ± 0.07

6.829 ± 0.102

6.536 ± 0.155

3.704 ± 0.086

5.908 ± 0.176

1.885 ± 0.086

7.192 ± 0.143

6.723 ± 0.264

9.937 ± 0.194

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.493 ± 0.094

6.254 ± 0.122

3.399 ± 0.111

2.53 ± 0.096

4.029 ± 0.126

7.349 ± 0.135

6.91 ± 0.173

7.049 ± 0.156

0.856 ± 0.055

4.131 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski