Helicobacter fennelliae MRY12-0050

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Helicobacteraceae; Helicobacter; Helicobacter fennelliae

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2446 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T1D0Z6|T1D0Z6_9HELI ABC transporter OS=Helicobacter fennelliae MRY12-0050 OX=1325130 GN=HFN_0007 PE=4 SV=1
MM1 pKa = 7.58CLAIPSKK8 pKa = 10.81VISIDD13 pKa = 3.21KK14 pKa = 10.47DD15 pKa = 3.4KK16 pKa = 10.47NTAIIEE22 pKa = 4.31TLGVCRR28 pKa = 11.84EE29 pKa = 4.05ASLDD33 pKa = 3.68LMQEE37 pKa = 4.03EE38 pKa = 4.99VAVGEE43 pKa = 4.16YY44 pKa = 10.58VLLHH48 pKa = 6.41IGYY51 pKa = 9.67IMSKK55 pKa = 9.79IDD57 pKa = 3.37TQSALEE63 pKa = 4.31SIALYY68 pKa = 9.42EE69 pKa = 4.22QIVEE73 pKa = 4.2DD74 pKa = 4.79MEE76 pKa = 5.51DD77 pKa = 3.46EE78 pKa = 4.83YY79 pKa = 11.08IDD81 pKa = 4.13EE82 pKa = 4.59EE83 pKa = 4.76YY84 pKa = 10.85KK85 pKa = 10.99

Molecular weight: 9.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T1DVC7|T1DVC7_9HELI Uncharacterized protein OS=Helicobacter fennelliae MRY12-0050 OX=1325130 GN=HFN_2055 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.09QPHH8 pKa = 5.34NTPKK12 pKa = 10.47KK13 pKa = 7.58RR14 pKa = 11.84THH16 pKa = 5.93GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.63TKK25 pKa = 9.91NGRR28 pKa = 11.84RR29 pKa = 11.84VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.41GRR39 pKa = 11.84KK40 pKa = 8.68RR41 pKa = 11.84LSVV44 pKa = 3.2

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2446

0

2446

637015

37

2887

260.4

29.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.822 ± 0.046

1.354 ± 0.022

5.164 ± 0.04

5.967 ± 0.047

5.317 ± 0.053

5.701 ± 0.057

2.137 ± 0.025

8.817 ± 0.048

7.96 ± 0.049

10.047 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.251 ± 0.023

5.536 ± 0.053

3.23 ± 0.029

4.312 ± 0.042

3.569 ± 0.029

7.178 ± 0.046

4.876 ± 0.039

5.069 ± 0.052

0.81 ± 0.016

3.874 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski