Sweet potato leaf curl Canary virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C5H6W7|C5H6W7_9GEMI C4 OS=Sweet potato leaf curl Canary virus OX=652718 GN=C4 PE=3 SV=1
MM1 pKa = 7.53PRR3 pKa = 11.84KK4 pKa = 9.77QGFRR8 pKa = 11.84VQAKK12 pKa = 10.14NIFLTYY18 pKa = 9.16PKK20 pKa = 10.23CSLSKK25 pKa = 10.46EE26 pKa = 4.04QALEE30 pKa = 3.79QLRR33 pKa = 11.84ATHH36 pKa = 6.44CPSDD40 pKa = 3.38KK41 pKa = 11.01LFIRR45 pKa = 11.84VSQEE49 pKa = 3.19KK50 pKa = 9.64HH51 pKa = 4.73QDD53 pKa = 3.27GSLHH57 pKa = 5.85LHH59 pKa = 5.88VLIQFKK65 pKa = 10.82GKK67 pKa = 10.71AEE69 pKa = 4.33FKK71 pKa = 10.61NPRR74 pKa = 11.84HH75 pKa = 6.15FDD77 pKa = 2.89LHH79 pKa = 6.48HH80 pKa = 6.64PHH82 pKa = 6.75NSSQFHH88 pKa = 7.06PNFQAAKK95 pKa = 9.29SSSDD99 pKa = 3.13VKK101 pKa = 11.23SYY103 pKa = 10.8IEE105 pKa = 4.51KK106 pKa = 10.92DD107 pKa = 3.01GDD109 pKa = 3.73YY110 pKa = 10.84LDD112 pKa = 3.69WGEE115 pKa = 3.93FQIDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 3.91AAAEE136 pKa = 3.92ALNAGSKK143 pKa = 9.5EE144 pKa = 3.68AALQIIRR151 pKa = 11.84EE152 pKa = 4.21KK153 pKa = 11.03LPEE156 pKa = 4.12KK157 pKa = 11.02YY158 pKa = 9.91IFQYY162 pKa = 10.91HH163 pKa = 5.84NLVSNLDD170 pKa = 4.36RR171 pKa = 11.84IFSPPPAVYY180 pKa = 10.25CSPFSSSSFNNVPDD194 pKa = 5.62IISDD198 pKa = 3.84WAAEE202 pKa = 3.97NVMDD206 pKa = 5.1SAARR210 pKa = 11.84PDD212 pKa = 3.59RR213 pKa = 11.84PISIVIEE220 pKa = 4.52GPSRR224 pKa = 11.84IGKK227 pKa = 6.21TVWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.09VYY251 pKa = 10.85SNSAWYY257 pKa = 10.34NVIDD261 pKa = 4.95DD262 pKa = 4.8VNPQYY267 pKa = 11.15LKK269 pKa = 10.65HH270 pKa = 6.26FKK272 pKa = 10.69EE273 pKa = 4.43FMGAQKK279 pKa = 10.56DD280 pKa = 3.62WQSNCKK286 pKa = 9.38YY287 pKa = 10.21GKK289 pKa = 9.18PVQIKK294 pKa = 10.41GGIPTIFLCNPGEE307 pKa = 4.28GSSFKK312 pKa = 10.83LWLDD316 pKa = 3.35KK317 pKa = 11.08PEE319 pKa = 4.24QEE321 pKa = 5.18ALKK324 pKa = 10.84NWALKK329 pKa = 10.36NAIFCDD335 pKa = 3.79VQSPFWVQEE344 pKa = 4.15EE345 pKa = 4.6VSGAGAITRR354 pKa = 11.84SSEE357 pKa = 3.86EE358 pKa = 4.0GQEE361 pKa = 4.12EE362 pKa = 4.77SSS364 pKa = 3.48

Molecular weight: 40.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C5HU40|C5HU40_9GEMI Protein V2 OS=Sweet potato leaf curl Canary virus OX=652718 GN=V2 PE=3 SV=1
MM1 pKa = 7.03GHH3 pKa = 7.23CISMFSSSSRR13 pKa = 11.84GKK15 pKa = 10.24QSSKK19 pKa = 8.25THH21 pKa = 7.04DD22 pKa = 2.81ISTYY26 pKa = 9.71IIHH29 pKa = 6.3ITPPNSIQISRR40 pKa = 11.84QLSPPRR46 pKa = 11.84MSSHH50 pKa = 5.23TSRR53 pKa = 11.84RR54 pKa = 11.84TEE56 pKa = 3.95TTSTGVNSRR65 pKa = 11.84STADD69 pKa = 3.5LLEE72 pKa = 4.45EE73 pKa = 4.23VSRR76 pKa = 11.84LLTTLPQRR84 pKa = 11.84HH85 pKa = 5.46

Molecular weight: 9.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1127

85

364

187.8

21.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.324 ± 0.815

2.928 ± 0.775

4.969 ± 0.289

5.324 ± 0.704

4.17 ± 0.579

6.122 ± 0.398

3.549 ± 0.401

5.768 ± 0.534

6.034 ± 0.69

6.921 ± 0.934

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.218 ± 0.565

4.348 ± 0.554

6.122 ± 0.899

4.525 ± 0.647

7.453 ± 1.348

8.607 ± 1.399

5.235 ± 1.314

4.791 ± 0.95

1.775 ± 0.303

3.815 ± 0.619
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski