Vibrio phage SIO-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 114 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8CTC0|G8CTC0_9CAUD Uncharacterized protein OS=Vibrio phage SIO-2 OX=700512 GN=VPEG_00050 PE=4 SV=1
MM1 pKa = 7.32TKK3 pKa = 10.36VIYY6 pKa = 10.02DD7 pKa = 3.47YY8 pKa = 11.41QYY10 pKa = 11.25VNKK13 pKa = 10.07ANPFHH18 pKa = 6.52FVKK21 pKa = 10.84LSLDD25 pKa = 3.42GDD27 pKa = 4.11FVEE30 pKa = 5.96VGAEE34 pKa = 3.95DD35 pKa = 4.11GEE37 pKa = 4.58EE38 pKa = 3.98VSDD41 pKa = 5.98AILKK45 pKa = 10.9DD46 pKa = 3.6MVNMYY51 pKa = 10.61DD52 pKa = 3.06VDD54 pKa = 3.86GFSIEE59 pKa = 4.34DD60 pKa = 3.43LL61 pKa = 4.02

Molecular weight: 6.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8CT95|G8CT95_9CAUD Uncharacterized protein OS=Vibrio phage SIO-2 OX=700512 GN=VPEG_00025 PE=4 SV=1
MM1 pKa = 7.61IFFITQFSIAFVTIFLRR18 pKa = 11.84GVQTQNVVQGNYY30 pKa = 9.65KK31 pKa = 10.49GAFLTSIGMSVANVAFIGMVAVDD54 pKa = 4.09PWASFIPVALGAATGITSSMYY75 pKa = 9.53YY76 pKa = 10.1KK77 pKa = 10.23RR78 pKa = 11.84RR79 pKa = 11.84NMM81 pKa = 3.66

Molecular weight: 8.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

114

0

114

24890

61

1377

218.3

24.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.236 ± 0.378

1.117 ± 0.102

6.452 ± 0.164

6.545 ± 0.224

3.6 ± 0.15

5.962 ± 0.172

2.005 ± 0.14

5.958 ± 0.147

6.802 ± 0.259

9.462 ± 0.234

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.041 ± 0.101

5.115 ± 0.164

3.64 ± 0.172

3.407 ± 0.158

5.376 ± 0.21

6.734 ± 0.209

6.227 ± 0.175

6.922 ± 0.185

0.759 ± 0.073

3.64 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski