Shimia aestuarii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Shimia

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4052 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I4HDB6|A0A1I4HDB6_9RHOB N-(5'-phosphoribosyl)anthranilate isomerase OS=Shimia aestuarii OX=254406 GN=trpF PE=3 SV=1
MM1 pKa = 7.38TFIPYY6 pKa = 8.77LTFDD10 pKa = 4.03GTAEE14 pKa = 3.82AAMNFYY20 pKa = 11.18ADD22 pKa = 3.5VFQATEE28 pKa = 3.95IQIMRR33 pKa = 11.84YY34 pKa = 9.32SDD36 pKa = 3.57APADD40 pKa = 3.88EE41 pKa = 5.03GLPASDD47 pKa = 3.24RR48 pKa = 11.84VMHH51 pKa = 5.78AHH53 pKa = 7.07IMLGDD58 pKa = 3.82QCLMAADD65 pKa = 4.76GMPGTPVPAQDD76 pKa = 3.52SVSINHH82 pKa = 6.57PVPSVAVGQAIFDD95 pKa = 4.2KK96 pKa = 10.96LSDD99 pKa = 3.52GAEE102 pKa = 3.84IIMPYY107 pKa = 10.56GPTFYY112 pKa = 11.15APAFGMLRR120 pKa = 11.84DD121 pKa = 3.76KK122 pKa = 11.23FGTNWMIGVYY132 pKa = 10.07PEE134 pKa = 5.18DD135 pKa = 3.3EE136 pKa = 4.37TMPQQ140 pKa = 2.95

Molecular weight: 15.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4PXX9|A0A1I4PXX9_9RHOB Transcriptional regulator XRE family with cupin sensor OS=Shimia aestuarii OX=254406 GN=SAMN04488042_10698 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4052

0

4052

1267422

39

2253

312.8

34.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.766 ± 0.047

0.915 ± 0.013

6.088 ± 0.032

6.124 ± 0.039

3.834 ± 0.024

8.605 ± 0.038

2.16 ± 0.021

5.161 ± 0.027

3.469 ± 0.031

9.899 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.91 ± 0.018

2.762 ± 0.021

4.988 ± 0.027

3.178 ± 0.021

6.574 ± 0.037

5.131 ± 0.021

5.373 ± 0.025

7.357 ± 0.032

1.413 ± 0.017

2.294 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski