Podoviridae sp. ctda_1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; unclassified Schitoviridae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345ML53|A0A345ML53_9CAUD AAA domain protein OS=Podoviridae sp. ctda_1 OX=2675448 PE=4 SV=1
MM1 pKa = 7.37SRR3 pKa = 11.84PTFSLCCVIVTRR15 pKa = 11.84PTTKK19 pKa = 9.31EE20 pKa = 3.72TTMNAATNPLNGLAKK35 pKa = 8.79TLEE38 pKa = 4.16WFMTAKK44 pKa = 10.45PNPTPHH50 pKa = 7.39DD51 pKa = 3.57LQVQVGCHH59 pKa = 4.85IEE61 pKa = 4.02EE62 pKa = 4.28FEE64 pKa = 4.26EE65 pKa = 4.85FLTEE69 pKa = 5.27LEE71 pKa = 4.87LVDD74 pKa = 5.78DD75 pKa = 5.49DD76 pKa = 4.52YY77 pKa = 11.88QGKK80 pKa = 8.72HH81 pKa = 6.01LLGQAMLIIDD91 pKa = 4.68ALANHH96 pKa = 7.05LKK98 pKa = 10.63DD99 pKa = 3.46SQAVVLKK106 pKa = 10.95LEE108 pKa = 4.19ATTNVLDD115 pKa = 5.32AIADD119 pKa = 3.8GMVTGTGVAYY129 pKa = 9.73MLGMDD134 pKa = 4.94LLGGLDD140 pKa = 3.43EE141 pKa = 4.55VNRR144 pKa = 11.84SNFSKK149 pKa = 10.65FDD151 pKa = 4.43PDD153 pKa = 2.99TGLPVLKK160 pKa = 10.88SNGKK164 pKa = 8.58IGKK167 pKa = 8.3HH168 pKa = 5.3FSYY171 pKa = 10.11TKK173 pKa = 10.39PNLAPFLVLQQPVAADD189 pKa = 4.11DD190 pKa = 4.68DD191 pKa = 4.87SVIDD195 pKa = 3.73PATVVFDD202 pKa = 4.12ADD204 pKa = 3.6ADD206 pKa = 4.08EE207 pKa = 4.42VCIVDD212 pKa = 4.42AAADD216 pKa = 3.85AADD219 pKa = 4.63DD220 pKa = 4.13AASADD225 pKa = 3.93DD226 pKa = 4.38VGDD229 pKa = 3.96GSDD232 pKa = 3.44AEE234 pKa = 4.33EE235 pKa = 4.07AAEE238 pKa = 3.99ADD240 pKa = 3.65EE241 pKa = 5.05GGSEE245 pKa = 4.1GALAADD251 pKa = 4.31EE252 pKa = 4.57EE253 pKa = 4.79EE254 pKa = 4.49EE255 pKa = 4.21EE256 pKa = 4.18QDD258 pKa = 3.49ASAVV262 pKa = 3.52

Molecular weight: 27.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345ML17|A0A345ML17_9CAUD Uncharacterized protein OS=Podoviridae sp. ctda_1 OX=2675448 PE=4 SV=1
MM1 pKa = 7.39HH2 pKa = 7.52GDD4 pKa = 3.36SRR6 pKa = 11.84NEE8 pKa = 3.62AVTPRR13 pKa = 11.84SVHH16 pKa = 4.99AVRR19 pKa = 11.84RR20 pKa = 11.84GCGHH24 pKa = 6.57VAPSAHH30 pKa = 7.15RR31 pKa = 11.84SGQIHH36 pKa = 6.15LHH38 pKa = 6.41GGTDD42 pKa = 3.48HH43 pKa = 6.28QRR45 pKa = 11.84SVLPVRR51 pKa = 11.84LRR53 pKa = 11.84HH54 pKa = 4.16VHH56 pKa = 5.55HH57 pKa = 7.2RR58 pKa = 11.84GNHH61 pKa = 4.97RR62 pKa = 11.84MNEE65 pKa = 4.17TKK67 pKa = 9.97TPKK70 pKa = 10.06QLLDD74 pKa = 3.51EE75 pKa = 4.7ALCAIQQWISDD86 pKa = 4.0GFPEE90 pKa = 4.45TKK92 pKa = 10.05HH93 pKa = 6.43KK94 pKa = 10.41FSCRR98 pKa = 11.84VGICSAVRR106 pKa = 11.84RR107 pKa = 11.84YY108 pKa = 10.58LNRR111 pKa = 11.84EE112 pKa = 3.28GHH114 pKa = 6.38AFALIEE120 pKa = 5.12LIVDD124 pKa = 3.78DD125 pKa = 5.61LSMEE129 pKa = 4.41FEE131 pKa = 4.11GRR133 pKa = 11.84TYY135 pKa = 10.7PFNKK139 pKa = 10.12NGVEE143 pKa = 4.54GYY145 pKa = 10.32LSEE148 pKa = 4.99SINSNIYY155 pKa = 8.93RR156 pKa = 11.84NKK158 pKa = 10.33ARR160 pKa = 11.84RR161 pKa = 11.84QWLKK165 pKa = 10.92EE166 pKa = 3.61RR167 pKa = 11.84TQCLLSQQ174 pKa = 3.94

Molecular weight: 19.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

21189

80

3525

321.0

35.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.59 ± 0.676

0.883 ± 0.16

6.277 ± 0.224

6.239 ± 0.208

3.54 ± 0.183

7.485 ± 0.322

2.166 ± 0.203

5.078 ± 0.217

5.446 ± 0.339

8.15 ± 0.276

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.95 ± 0.165

4.568 ± 0.256

4.37 ± 0.243

4.498 ± 0.243

5.385 ± 0.261

5.998 ± 0.245

6.041 ± 0.241

7.225 ± 0.333

1.265 ± 0.133

2.846 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski