Lactuca sativa (Garden lettuce)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; campanulids; Asterales; Asteraceae; Cichorioideae; Cichorieae; Lactucinae;

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37927 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2J6L6Y0|A0A2J6L6Y0_LACSA Protein kinase domain-containing protein OS=Lactuca sativa OX=4236 GN=LSAT_8X142120 PE=4 SV=1
MM1 pKa = 7.94ASDD4 pKa = 4.32CKK6 pKa = 9.73QRR8 pKa = 11.84KK9 pKa = 7.89YY10 pKa = 10.77LIVYY14 pKa = 8.21IHH16 pKa = 6.45YY17 pKa = 10.78NGFFAPKK24 pKa = 9.47PLVYY28 pKa = 10.7LNDD31 pKa = 3.17VVAICDD37 pKa = 3.44VDD39 pKa = 4.89FGAMDD44 pKa = 4.43LKK46 pKa = 11.07EE47 pKa = 4.71FILFIEE53 pKa = 4.21KK54 pKa = 10.31LIEE57 pKa = 4.37GSCDD61 pKa = 2.75NVYY64 pKa = 10.76YY65 pKa = 8.33FTRR68 pKa = 11.84NEE70 pKa = 3.89PLAEE74 pKa = 4.88GIRR77 pKa = 11.84RR78 pKa = 11.84VGNDD82 pKa = 2.57ADD84 pKa = 3.64YY85 pKa = 11.5FEE87 pKa = 5.5FIEE90 pKa = 4.68MGYY93 pKa = 10.66NDD95 pKa = 4.2EE96 pKa = 5.46NGLKK100 pKa = 8.68MNVYY104 pKa = 9.44IDD106 pKa = 3.93HH107 pKa = 6.84KK108 pKa = 11.37NEE110 pKa = 4.77LVLDD114 pKa = 3.94WADD117 pKa = 3.58MEE119 pKa = 4.75VVEE122 pKa = 5.51DD123 pKa = 5.58DD124 pKa = 4.15EE125 pKa = 6.5GNDD128 pKa = 3.59YY129 pKa = 11.42DD130 pKa = 5.75QDD132 pKa = 4.2PDD134 pKa = 4.97DD135 pKa = 5.19DD136 pKa = 4.67NKK138 pKa = 11.29SQLSDD143 pKa = 4.63DD144 pKa = 3.5IPYY147 pKa = 10.25EE148 pKa = 4.2HH149 pKa = 7.15EE150 pKa = 4.49ADD152 pKa = 4.54DD153 pKa = 5.12YY154 pKa = 11.59IPSLDD159 pKa = 3.81KK160 pKa = 11.12TIGDD164 pKa = 3.8

Molecular weight: 19.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2J6K3Q8|A0A2J6K3Q8_LACSA Endoplasmic reticulum transmembrane protein OS=Lactuca sativa OX=4236 GN=LSAT_2X17460 PE=3 SV=1
MM1 pKa = 7.55ARR3 pKa = 11.84QPKK6 pKa = 8.62RR7 pKa = 11.84KK8 pKa = 9.32NMKK11 pKa = 9.46RR12 pKa = 11.84KK13 pKa = 9.22RR14 pKa = 11.84NTRR17 pKa = 11.84AVRR20 pKa = 11.84MGAEE24 pKa = 3.69KK25 pKa = 10.52KK26 pKa = 9.83ARR28 pKa = 11.84KK29 pKa = 8.88RR30 pKa = 11.84LKK32 pKa = 9.5IRR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.58RR37 pKa = 11.84RR38 pKa = 11.84TATRR42 pKa = 11.84LVVRR46 pKa = 11.84VEE48 pKa = 3.45RR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84KK53 pKa = 10.02RR54 pKa = 11.84ILKK57 pKa = 9.55IKK59 pKa = 10.18RR60 pKa = 11.84IQKK63 pKa = 9.72SS64 pKa = 3.06

Molecular weight: 8.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

37926

1

37927

13441818

50

5315

354.4

39.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.951 ± 0.011

1.8 ± 0.007

5.428 ± 0.008

6.419 ± 0.014

4.314 ± 0.009

6.477 ± 0.012

2.469 ± 0.006

5.75 ± 0.01

6.414 ± 0.014

9.597 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.624 ± 0.006

4.78 ± 0.01

4.786 ± 0.014

3.509 ± 0.009

4.939 ± 0.01

8.726 ± 0.013

5.188 ± 0.008

6.614 ± 0.01

1.292 ± 0.005

2.895 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski