Verticillium nonalfalfae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Glomerellales; Plectosphaerella

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9427 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M9YK08|A0A3M9YK08_9PEZI Rho-GAP domain-containing protein OS=Verticillium nonalfalfae OX=1051616 GN=D7B24_005929 PE=4 SV=1
MM1 pKa = 7.69RR2 pKa = 11.84ASILPILALPRR13 pKa = 11.84LALAQNSDD21 pKa = 3.46RR22 pKa = 11.84PRR24 pKa = 11.84CVATCITNNLMSSHH38 pKa = 7.21CDD40 pKa = 3.12GDD42 pKa = 3.98EE43 pKa = 3.8QGAALDD49 pKa = 4.02EE50 pKa = 4.63CTCAALSGSPMIACIRR66 pKa = 11.84DD67 pKa = 3.85CTPADD72 pKa = 3.38QGQYY76 pKa = 10.45AAQLPGLCRR85 pKa = 11.84DD86 pKa = 3.76RR87 pKa = 11.84LLPDD91 pKa = 3.62AEE93 pKa = 4.43GASGGGGGGGHH104 pKa = 7.58DD105 pKa = 5.83DD106 pKa = 5.99DD107 pKa = 7.72DD108 pKa = 7.49DD109 pKa = 7.73DD110 pKa = 6.93DD111 pKa = 5.45DD112 pKa = 4.11QTTTTTAAILTTTMTSPSATTAAGGDD138 pKa = 3.77TATGTDD144 pKa = 3.01AAAAEE149 pKa = 4.66TEE151 pKa = 4.44TPAAGVGNEE160 pKa = 4.12VPVALAAGLFAALLLL175 pKa = 4.36

Molecular weight: 17.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M9Y844|A0A3M9Y844_9PEZI Transcription initiation factor IIF subunit alpha OS=Verticillium nonalfalfae OX=1051616 GN=D7B24_007204 PE=3 SV=1
MM1 pKa = 7.94PILRR5 pKa = 11.84SSQPRR10 pKa = 11.84RR11 pKa = 11.84TRR13 pKa = 11.84HH14 pKa = 4.15TTTKK18 pKa = 9.78TSRR21 pKa = 11.84SNGGLFSRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84QPARR35 pKa = 11.84TTHH38 pKa = 5.14TTTTTTTTHH47 pKa = 6.21RR48 pKa = 11.84QHH50 pKa = 7.12AGATAPRR57 pKa = 11.84GGFFSRR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84GPVVHH70 pKa = 6.33HH71 pKa = 5.68QQRR74 pKa = 11.84KK75 pKa = 8.1PSMGDD80 pKa = 3.22KK81 pKa = 10.49ISGAMLRR88 pKa = 11.84LKK90 pKa = 10.8GSLTRR95 pKa = 11.84RR96 pKa = 11.84PGQKK100 pKa = 9.9AAGTRR105 pKa = 11.84RR106 pKa = 11.84MNGTDD111 pKa = 2.82GRR113 pKa = 11.84GSHH116 pKa = 5.61RR117 pKa = 11.84TRR119 pKa = 11.84RR120 pKa = 11.84WW121 pKa = 2.82

Molecular weight: 13.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9427

0

9427

4738719

50

7576

502.7

55.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.788 ± 0.026

1.165 ± 0.014

5.948 ± 0.019

5.967 ± 0.024

3.621 ± 0.016

7.222 ± 0.028

2.405 ± 0.011

4.459 ± 0.014

4.53 ± 0.025

8.684 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.18 ± 0.011

3.443 ± 0.014

6.246 ± 0.034

3.963 ± 0.019

6.222 ± 0.021

7.796 ± 0.022

6.006 ± 0.018

6.31 ± 0.018

1.456 ± 0.01

2.586 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski