Rhizobium phage RHEph09

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Cuernavacavirus; Rhizobium virus RHEph09

Average proteome isoelectric point is 7.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L7TNV8|L7TNV8_9CAUD Uncharacterized protein OS=Rhizobium phage RHEph09 OX=1220716 GN=RHEph09_gp036 PE=4 SV=1
MM1 pKa = 7.22EE2 pKa = 4.62QVCVWPDD9 pKa = 3.35GTWCYY14 pKa = 10.86KK15 pKa = 10.92DD16 pKa = 4.23EE17 pKa = 4.6LPEE20 pKa = 4.0MTHH23 pKa = 6.25MSDD26 pKa = 3.84DD27 pKa = 4.25YY28 pKa = 11.55YY29 pKa = 10.13ITSYY33 pKa = 11.63DD34 pKa = 3.43EE35 pKa = 4.43ALEE38 pKa = 4.27LGEE41 pKa = 5.26IKK43 pKa = 10.64DD44 pKa = 3.65

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L7TN95|L7TN95_9CAUD Uncharacterized protein OS=Rhizobium phage RHEph09 OX=1220716 GN=RHEph09_gp034 PE=4 SV=1
MM1 pKa = 7.34SNEE4 pKa = 3.86IVRR7 pKa = 11.84LTAKK11 pKa = 9.66QVPVVKK17 pKa = 10.29QAILSRR23 pKa = 11.84RR24 pKa = 11.84QGNRR28 pKa = 11.84CPLCHH33 pKa = 7.12RR34 pKa = 11.84GLTVLDD40 pKa = 3.66GCMDD44 pKa = 4.13HH45 pKa = 7.31DD46 pKa = 4.22HH47 pKa = 5.37VTGRR51 pKa = 11.84VRR53 pKa = 11.84GILCRR58 pKa = 11.84GCNGAEE64 pKa = 3.88GKK66 pKa = 9.92IKK68 pKa = 10.69NAFVRR73 pKa = 11.84YY74 pKa = 9.48GGGLRR79 pKa = 11.84TEE81 pKa = 4.54LVPFLRR87 pKa = 11.84NLADD91 pKa = 3.45YY92 pKa = 11.22LEE94 pKa = 5.04YY95 pKa = 10.89YY96 pKa = 8.56QQNPNNFLYY105 pKa = 10.46HH106 pKa = 5.35LHH108 pKa = 6.7RR109 pKa = 11.84NEE111 pKa = 3.85DD112 pKa = 3.44EE113 pKa = 4.41KK114 pKa = 11.23RR115 pKa = 11.84VLRR118 pKa = 11.84NKK120 pKa = 10.09RR121 pKa = 11.84ARR123 pKa = 11.84KK124 pKa = 9.24ARR126 pKa = 11.84AARR129 pKa = 11.84KK130 pKa = 7.41TKK132 pKa = 10.6

Molecular weight: 15.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

14119

38

1218

231.5

25.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.965 ± 0.462

0.885 ± 0.154

6.254 ± 0.27

5.574 ± 0.301

3.272 ± 0.217

7.699 ± 0.298

2.189 ± 0.211

5.418 ± 0.264

5.73 ± 0.336

7.678 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.131 ± 0.178

4.391 ± 0.225

3.917 ± 0.147

4.554 ± 0.245

5.921 ± 0.276

5.843 ± 0.278

5.836 ± 0.34

6.488 ± 0.26

1.679 ± 0.099

3.577 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski