Pelagibacter phage HTVC120P

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; unclassified Autographiviridae

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y1NSZ1|A0A4Y1NSZ1_9CAUD Uncharacterized protein OS=Pelagibacter phage HTVC120P OX=2283021 GN=P120_gp33 PE=4 SV=1
MM1 pKa = 7.47ANSFVRR7 pKa = 11.84YY8 pKa = 7.29TGNGSTTAYY17 pKa = 9.9SISYY21 pKa = 8.14TYY23 pKa = 10.76RR24 pKa = 11.84DD25 pKa = 3.59AADD28 pKa = 5.02LIVSVNGVATTAYY41 pKa = 9.98SLNAAGTTLTFDD53 pKa = 3.62SAPASSSAIEE63 pKa = 4.0IRR65 pKa = 11.84RR66 pKa = 11.84KK67 pKa = 8.24TSQTTRR73 pKa = 11.84LTDD76 pKa = 3.47YY77 pKa = 11.08AAGSVLTEE85 pKa = 4.12NDD87 pKa = 3.84LDD89 pKa = 4.08TDD91 pKa = 3.79STQAFFMGQEE101 pKa = 4.68AIDD104 pKa = 4.37DD105 pKa = 4.34ANDD108 pKa = 3.5VIKK111 pKa = 10.73ISSTDD116 pKa = 3.76FQFNAINKK124 pKa = 7.92QIRR127 pKa = 11.84NVANPTSNQDD137 pKa = 3.03VATKK141 pKa = 10.09HH142 pKa = 5.43YY143 pKa = 10.9LEE145 pKa = 4.34NTFLTTANKK154 pKa = 8.32TALTTINANIANIIAVNNNSTNINSAVSNATNINTVATNIGSVNTVAADD203 pKa = 3.22IAKK206 pKa = 10.13VIEE209 pKa = 4.24VANDD213 pKa = 3.25LQEE216 pKa = 4.33AVSEE220 pKa = 4.55VEE222 pKa = 4.42TVADD226 pKa = 4.55DD227 pKa = 4.36LNEE230 pKa = 4.13ATSEE234 pKa = 3.92IDD236 pKa = 3.39TVATSITNVDD246 pKa = 3.46TVGNNIANVNAVAGNATNINAVNSNSSNINTVAGNNTNINTVAGANANITTLAGINANITTVAGINSNVSTVAGISSDD324 pKa = 3.44VTAVAGISSDD334 pKa = 3.49VQAVEE339 pKa = 4.18NIKK342 pKa = 11.3ANVTTVAGISSAVSNVSGISSAVSAVNSNSSNINAVNSNSANINTVASNNSNISTVAGVSSDD404 pKa = 3.32VTTVAGIASDD414 pKa = 3.68VSAVEE419 pKa = 4.64NIASNVTTVAGMSTAINTVNSNATNVNAVGGAIANVNNVGGSIANVNTVATNLASVNAFGEE480 pKa = 4.67TYY482 pKa = 10.14RR483 pKa = 11.84IASSAPTSSLNSGDD497 pKa = 4.82LYY499 pKa = 11.44FNTSTNVLNVYY510 pKa = 9.07GASGWQNAGSSVNGTSEE527 pKa = 3.99RR528 pKa = 11.84YY529 pKa = 9.77KK530 pKa = 10.63FVASGTPTTLTGNDD544 pKa = 3.41ANGNTLAYY552 pKa = 9.83DD553 pKa = 3.44AGFIDD558 pKa = 5.23VYY560 pKa = 11.43LNGIKK565 pKa = 9.16MVNGTDD571 pKa = 3.42VTVTSGSSIVFASALTNGDD590 pKa = 2.73IVEE593 pKa = 4.38AVTFGTFSVANMNASNLTSGTVPDD617 pKa = 3.78ARR619 pKa = 11.84ITGTYY624 pKa = 9.16TGITGLDD631 pKa = 3.22LTDD634 pKa = 3.31NSKK637 pKa = 10.17IRR639 pKa = 11.84LGTDD643 pKa = 2.78QMLEE647 pKa = 4.15LLEE650 pKa = 4.38HH651 pKa = 5.22TQEE654 pKa = 4.31LQDD657 pKa = 3.46

Molecular weight: 66.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y1NSY3|A0A4Y1NSY3_9CAUD Uncharacterized protein OS=Pelagibacter phage HTVC120P OX=2283021 GN=P120_gp35 PE=4 SV=1
MM1 pKa = 7.74AKK3 pKa = 10.23NGLYY7 pKa = 10.91ANIHH11 pKa = 6.08KK12 pKa = 10.25KK13 pKa = 9.21RR14 pKa = 11.84ARR16 pKa = 11.84IKK18 pKa = 10.26SGSGEE23 pKa = 4.08KK24 pKa = 9.51MRR26 pKa = 11.84TAGTKK31 pKa = 9.77GRR33 pKa = 11.84PTTAQFKK40 pKa = 10.05RR41 pKa = 11.84AAKK44 pKa = 8.06TAKK47 pKa = 10.08SS48 pKa = 3.41

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

13103

44

875

247.2

27.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.983 ± 0.451

0.885 ± 0.169

5.899 ± 0.254

6.075 ± 0.396

4.098 ± 0.202

6.502 ± 0.384

1.702 ± 0.164

6.617 ± 0.209

8.113 ± 0.652

8.136 ± 0.387

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.427 ± 0.25

6.724 ± 0.507

3.167 ± 0.218

3.969 ± 0.274

3.976 ± 0.329

7.243 ± 0.539

7.159 ± 0.554

5.983 ± 0.382

1.183 ± 0.127

3.16 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski